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1.14.12.10: benzoate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about benzoate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.10

Reaction

benzoate
+
NADH
+
H+
+
O2
=
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate
+
NAD+

Synonyms

BDO, BenA, benABC, benzoate 1,2-dioxygenase, benzoate 1,2-dioxygenase oxygenase, benzoate 1,2-dioxygenase system, benzoate dioxygenase, benzoate hydroxylase, benzoic hydroxylase, BZDO, BZDO enzyme, EC 1.13.99.2, oxygenase, benzoate 1,2-di-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.10 benzoate 1,2-dioxygenase

Cloned

Cloned on EC 1.14.12.10 - benzoate 1,2-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Pseudomonas putida CL01 as His-tag fusion protein
-
expression of presumed dioxygenase component in Escherichia coli
-
The genes benABC encoding benzoate dioxygenase are cloned into plasmid pSYM01 which harboring a cis-diol dehydrogenase knockout and overexpressed in Pseudomona putida KTSY01.
-
when expressed in Escherichia coli, benzoate 1,2-dioxygenase transforms benzoate into cis-benzoate dihydrodiol
-