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6.6
(S)-2-Hydroxy-2-phenylacetate
mutant A95G, pH 7.0, 25°C
0.0033 - 15.8
(S)-mandelic acid
11 - 510
2-hydroxybutanoate
1.25 - 51.7
2-hydroxypentanoate
12
L-alpha-hydroxy-beta-methylvalerate
mutant A95G, pH 7.0, 25°C
5
(S)-lactate
mutant Y191A, pH 6.5, 20°C
5
(S)-lactate
mutant Y215H, pH 6.5, 37°C
7.5
(S)-lactate
mutant Y191L, pH 6.5, 20°C
22
(S)-lactate
mutant Y191F, pH 6.5, 20°C
22
(S)-lactate
mutant Y215F, pH 6.5, 37°C
31
(S)-lactate
pH 7.0, 4°C
88
(S)-lactate
wild-type, pH 6.5, 37°C
88
(S)-lactate
wild-type, pH 6.5, 20°C
140
(S)-lactate
mutant A95G, pH 6.5, 20°C
140
(S)-lactate
wild-type, pH 6.5, 20°C
280
(S)-lactate
pH 7.0, 25°C
0.0033
(S)-mandelic acid
wild-type, pH 7.0, 25°C
15.8
(S)-mandelic acid
mutant A95G, pH 7.0, 25°C
11
2-hydroxybutanoate
wild-type, pH 7.0, 25°C
510
2-hydroxybutanoate
mutant A95G, pH 7.0, 25°C
1.25
2-hydroxypentanoate
wild-type, pH 7.0, 25°C
51.7
2-hydroxypentanoate
mutant A95G, pH 7.0, 25°C
0.87
glycerate
wild-type, pH 7.0, 25°C
31.3
glycerate
mutant A95G, pH 7.0, 25°C
250
L-lactate
mutant A95G, pH 7.0, 25°C
283
L-lactate
wild-type, pH 7.0, 25°C
1.1.3.2 L-lactate oxidase
results (
results (
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