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0.14
(R)-lactate
Gram-negative soil bacterium
-
-
0.3 - 20
(S)-mandelic acid
18 - 27
2-hydroxybutanoate
5.5 - 10
2-hydroxypentanoate
0.2
Lactate
Gram-negative soil bacterium
-
-
additional information
additional information
detailed analysis of reductive and oxidative half-reaction
-
0.00034
(S)-lactate
pH 7.0, 4°C
0.00094
(S)-lactate
pH 7.0, 25°C
0.08
(S)-lactate
mutant Y191F, pH 6.5, 20°C
0.13
(S)-lactate
mutant Y215F, pH 6.5, 37°C
0.157
(S)-lactate
pH 7.0, 37°C
0.43
(S)-lactate
pH 7.0, 37°C
0.5
(S)-lactate
wild-type, pH 6.5, 37°C
0.5
(S)-lactate
wild-type, pH 6.5, 20°C
0.52
(S)-lactate
mutant Y215H, pH 6.5, 37°C
0.56
(S)-lactate
pH 7.0, 25°C, wild-type enzyme
1.5
(S)-lactate
mutant Y191L, pH 6.5, 20°C
46
(S)-lactate
mutant Y191A, pH 6.5, 20°C
0.3
(S)-mandelic acid
wild-type, pH 7.0, 25°C
20
(S)-mandelic acid
mutant A95G, pH 7.0, 25°C
18
2-hydroxybutanoate
wild-type, pH 7.0, 25°C
27
2-hydroxybutanoate
mutant A95G, pH 7.0, 25°C
5.5
2-hydroxypentanoate
wild-type, pH 7.0, 25°C
10
2-hydroxypentanoate
mutant A95G, pH 7.0, 25°C
5
glycerate
wild-type, pH 7.0, 25°C
53
glycerate
mutant A95G, pH 7.0, 25°C
0.039
L-lactate
pH 8.0, 30°C
0.081
L-lactate
pH 8.0, 30°C
0.87
L-lactate
wild-type, pH 7.0, 25°C
0.94
L-lactate
wild-type, pH 7.0, 25°C
6.75
L-lactate
mutant A95G, pH 7.0, 25°C
7.5
L-lactate
mutant R181K, pH 7.0, 25°C
103
L-lactate
mutant R181M, pH 7.0, 25°C
0.024
O2
mutant Y215F, pH 6.5, 37°C
0.029
O2
mutant R181K, pH 7.0, 25°C
0.03
O2
mutant R181M, pH 7.0, 25°C
0.051
O2
wild-type, pH 6.5, 37°C
0.062
O2
mutant Y215H, pH 6.5, 37°C
0.16
O2
wild-type, pH 7.0, 25°C
1.1.3.2 L-lactate oxidase
results (
results (
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