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1.1.1.370: scyllo-inositol 2-dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about scyllo-inositol 2-dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.1.1.370

Reaction

scyllo-Inositol
+
NAD+
=
2,4,6/3,5-pentahydroxycyclohexanone
 +
NADH
 +
H+

Synonyms

DegA, IgdA, IolX, LgdA, NAD+-dependent SI dehydrogenase, Pl-scyllo-IDH, scyllo-inositol dehydrogenase, yisS

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.370 scyllo-inositol 2-dehydrogenase (NAD+)

Crystallization

Crystallization on EC 1.1.1.370 - scyllo-inositol 2-dehydrogenase (NAD+)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified His-tagged wild-type and selenomethionine-labeled enzyme in apoform and in a ternary complex with NAD+ and L-glucono-1,5-lactone, and in complex with myo-inositol and scyllo-inosose, hanging-drop vapor-diffusion method, mixing of 0.002 ml of protein solution with 0.02 ml of reservoir solution containing 0.1 M sodium acetate pH 4.8, and 12% PEG 3350, 20°C, crystals are soaked for one minute with 25 mM of NAD+ and 100 mM substrate in the reservoir solution, X-ray diffraction structure deteramintion and analysis at 1.75-2.3 A resolution, the structures complexed with myo-inositol/NAD+ and scyllo-inosose/NAD+ are solved at 2.3 A and 2.0 A resolution, respectively. Molecular replacement using the Se-Met-structure as the search model, modeling. The crystal is soaked with L-glucose and the resulting structure is determined