1.1.1.360: glucose/galactose 1-dehydrogenase

This is an abbreviated version!
For detailed information about glucose/galactose 1-dehydrogenase, go to the full flat file.

Reaction

D-galactopyranose
+
NADP+
=
D-galactono-1,5-lactone
+
NADPH
+
H+

Synonyms

dual-specific glucose/galactose dehydrogenase, GdhA, GlcDH, glucose (galactose) dehydrogenase, glucose/galactose dehydrogenase, TvGlcD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.360 glucose/galactose 1-dehydrogenase

Engineering

Engineering on EC 1.1.1.360 - glucose/galactose 1-dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D306N
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; improvement of the reactivity towards D-xylose can not be achieved, while the mutations reduces the D-glucose oxidation activity
E156Q
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; improvement of the reactivity towards D-xylose can not be achieved, while the mutations reduces the D-glucose oxidation activity
E296H
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; improvement of the reactivity towards D-xylose can not be achieved, while the mutations reduces the D-glucose oxidation activity
K159A
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; improvement of the reactivity towards D-xylose can not be achieved, while the mutations reduces the D-glucose oxidation activity
K159A/N160D
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; improvement of the reactivity towards D-xylose can not be achieved, while the mutations reduces the D-glucose oxidation activity
N160D
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; improvement of the reactivity towards D-xylose can not be achieved, while the mutations reduces the D-glucose oxidation activity
T277F
crystallization of the T277F mutant enzyme in a binary complex with NADP and in a ternary complex with D-glucose and nicotinic acid adenine dinucleotide phosphate, mutation reduces the D-glucose oxidation activity, the Vmax for the T277F mutant is about 12% of that for the wild-type enzyme; crystal structure of the T277F mutant enzyme in a binary complex with NADP+ and in a ternary complex with D-glucose and nicotinic acid adenine dinucleotide phosphate. The Vmax for the T277F mutant is about 12% of that for the wild-type enzyme
V93N
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; the Vmax value for D-glucose oxidation by the purified V93N mutant is about 71% of the Vmax value for the wild-type enzyme. Enhancement of the activity towards D-xylose is not observed
V93N/D306N
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; improvement of the reactivity towards D-xylose can not be achieved, while the mutations reduces the D-glucose oxidation activity
V93N/E156Q/K159A/N160D/D306N/E296H
activity is completely abolished; mutation completely abolished the activity of the enzyme
V93N/K159A
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; improvement of the reactivity towards D-xylose can not be achieved, while the mutations reduces the D-glucose oxidation activity
V93N/K159A/N160D
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; improvement of the reactivity towards D-xylose can not be achieved, while the mutations reduces the D-glucose oxidation activity
V93N/N160D
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; improvement of the reactivity towards D-xylose can not be achieved, while the mutations reduces the D-glucose oxidation activity
D306N
-
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity
-
K159A
-
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; improvement of the reactivity towards D-xylose can not be achieved, while the mutations reduces the D-glucose oxidation activity
-
N160D
-
enhancement of the activity towards D-xylose is not observed, mutation reduces the D-glucose oxidation activity; improvement of the reactivity towards D-xylose can not be achieved, while the mutations reduces the D-glucose oxidation activity
-