1.1.1.100: 3-oxoacyl-[acyl-carrier-protein] reductase

This is an abbreviated version!
For detailed information about 3-oxoacyl-[acyl-carrier-protein] reductase, go to the full flat file.

Word Map on EC 1.1.1.100

Reaction

a (3R)-3-hydroxyacyl-[acyl-carrier protein]
+
NADP+
=
a 3-oxoacyl-[acyl-carrier protein]
+
NADPH
+
H+

Synonyms

3-ketoacyl acyl carrier protein reductase, 3-ketoacyl-(acyl-carrier-protein) reductase, 3-ketoacyl-ACP reductase, 3-ketoacyl-ACP reductase/3R-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl-ACP(CoA) reductase, 3-ketoacyl-acyl carrier protein reductase, 3-ketoacyl-thioester reductase, 3-ketoacyl-[acyl-carrier-protein] reductase, 3-oxo-acyl-ACP reductase, 3-oxoacyl-(acyl carrier protein) reductase, 3-oxoacyl-(acyl-carrier-protein) reductase, 3-oxoacyl-ACP reductase, 3-oxoacyl-AcpM reductase, 3-oxoacyl-thioester reductase, 3-oxoacyl-[ACP]reductase, ACP reductase, beta-ketoacyl acyl carrier protein (ACP) reductase, beta-ketoacyl acyl carrier protein reductase, beta-ketoacyl reductase, beta-ketoacyl thioester reductase, beta-ketoacyl-(acyl carrier protein) reductase, beta-ketoacyl-ACP reductase, beta-ketoacyl-acyl carrier protein reductase, beta-ketoacyl-[acyl carrier protein] reductase, beta-ketoacyl-[acyl-carrier protein] (ACP) reductase, BKR, FabG, FabG1, fabG4, KACPR, KAR, KCR1, KCR2, ketoacyl-acyl carrier protein reductase, MabA, MSMEG_6753, NADPH-dependent acetoacetyl coenzyme A reductase, NADPH-specific 3-oxoacyl-[acylcarrier protein]reductase, OAR, OAR1, Oar1p, PHA-specific acetoacetyl-CoA reductase, PhaB, polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase, reductase, 3-oxoacyl-[acyl carrier protein], RhlG

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.100 3-oxoacyl-[acyl-carrier-protein] reductase

Purification

Purification on EC 1.1.1.100 - 3-oxoacyl-[acyl-carrier-protein] reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by gel filtration and ion exchange chromatography, to homogeneity
by nickel affinity chromatography
-
by nickel chelation affinity chromatography
by nickel-affinity chromatography
-
by sonication, centrifugation and on Ni-NTA column
His-Bind resin column chromatography and Superdex 200 pg gel filtration
Ni-NTA agarose column chromatography and Superdex 200 pg gel filtration
-
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni-NTA resin column chromatography and Superdex 200 gel filtration
nickel Sepharose column chromatography and ENrich SEC 650 gel filtration
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) solubilized from inclusion bodies and refolded is further purified by nickel affinity chromatography
-
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography
recombinant His10-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant protein
-
recombinant protein, modified purification protocol
-
using Ni-NTA chromatography
wild-type and mutants purified to homogeneity using an Ni2+-nitrilotriacetic acid affinity column
-