EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
2.7.1.11 | -999 |
- |
more |
kcat increases with pH, addition of GDP increases Kcat at constant pH |
640489 |
2.7.1.11 | 0.015 |
- |
fructose 6-phosphate |
pH 8.0, 8.5°C, T125A mutant PFK |
640517 |
2.7.1.11 | 0.016 |
- |
D-fructose 6-phosphate |
pH 7.2, 30°C, cosubstrate gamma-thio-ATP |
640526 |
2.7.1.11 | 0.037 |
- |
ATP |
wild type enzyme, in the presence of Mn2+, in 0.05 M Tris-HCl, pH 8.2, temperature not specified in the publication |
721420 |
2.7.1.11 | 0.24 |
- |
ATP |
mutant enzyme E190Q, in the presence of Mg2+, in 0.05 M Tris-HCl, pH 8.2, temperature not specified in the publication |
721420 |
2.7.1.11 | 0.25 |
- |
sedoheptulose 7-phosphate |
pH 7.0, 55°C |
761519 |
2.7.1.11 | 0.3 |
- |
fructose 6-phosphate |
pH 8.5, 30°C, R72H mutant PFK |
640490 |
2.7.1.11 | 0.303 |
- |
fructose 6-phosphate |
pH 8.0, 8.5°C, R72E mutant PFK |
640517 |
2.7.1.11 | 1.2 |
- |
D-fructose 6-phosphate |
pH 7.0, 55°C |
761519 |
2.7.1.11 | 3.2 |
- |
fructose 1,6-bisphosphate |
reverse raction, recombinant enzyme |
640484 |