Literature summary for 2.7.1.11 extracted from

Koendjbiharie, J.G.; Hon, S.; Pabst, M.; Hooftman, R.; Stevenson, D.M.; Cui, J.; Amador-Noguez, D.; Lynd, L.R.; Olson, D.G.; van Kranenburg, R.
The pentose phosphate pathway of cellulolytic clostridia relies on 6-phosphofructokinase instead of transaldolase (2020), J. Biol. Chem., 295, 1867-1878 .

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli
overexpression in Escherichia coli	Pseudoclostridium thermosuccinogenes

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.065	-	D-fructose 6-phosphate	pH 7.0, 55°C	Escherichia coli
0.688	-	D-fructose 6-phosphate	pH 7.0, 55°C	Pseudoclostridium thermosuccinogenes
2.5	-	sedoheptulose 7-phosphate	pH 7.0, 55°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A796	-	-
Escherichia coli BW25113	P0A796	-	-
Pseudoclostridium thermosuccinogenes	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli
-	Pseudoclostridium thermosuccinogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-fructose 6-phosphate	-	Escherichia coli	ADP + D-fructose 1,6-bisphosphate	-	?
ATP + D-fructose 6-phosphate	the enzyme shows an extremely high degree of cooperative binding, resulting in a virtual on/off switch for substrate concentrations near its K1/2 value	Pseudoclostridium thermosuccinogenes	ADP + D-fructose 1,6-bisphosphate	-	?
ATP + D-fructose 6-phosphate	-	Escherichia coli BW25113	ADP + D-fructose 1,6-bisphosphate	-	?
ATP + sedoheptulose 7-phosphate	-	Escherichia coli	ADP + sedoheptulose 1,7-bisphosphate	-	?
ATP + sedoheptulose 7-phosphate	-	Escherichia coli BW25113	ADP + sedoheptulose 1,7-bisphosphate	-	?
GTP + D-fructose 6-phosphate	-	Pseudoclostridium thermosuccinogenes	GDP + D-fructose 1,6-bisphosphate	-	?

Synonyms

Synonyms	Comment	Organism
A/GTP-PFK	-	Pseudoclostridium thermosuccinogenes
PFKA	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.25	-	sedoheptulose 7-phosphate	pH 7.0, 55°C	Escherichia coli
1.2	-	D-fructose 6-phosphate	pH 7.0, 55°C	Escherichia coli
41	-	D-fructose 6-phosphate	pH 7.0, 55°C	Pseudoclostridium thermosuccinogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.15	-	sedoheptulose 7-phosphate	pH 7.0, 55°C	Escherichia coli
19	-	D-fructose 6-phosphate	pH 7.0, 55°C	Escherichia coli
59	-	D-fructose 6-phosphate	pH 7.0, 55°C	Pseudoclostridium thermosuccinogenes

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Release 2023.1 (January 2023)