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Literature summary for 2.7.1.11 extracted from

  • Zheng, R.L.; Kemp, R.G.
    The mechanism of ATP inhibition of wild type and mutant phosphofructo-1-kinase from Escherichia coli (1992), J. Biol. Chem., 267, 23640-23645.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
GDP
-
Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant PFK in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
I126A 600fold lower affinity for fructose 6-phosphate than wild-type, modestly lower Kcat than wild-type, greater ATP inhibition at pH 6.0 and 7.0 than at pH 8.0 Escherichia coli
R72H almost no PFK activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
ATP at alkaline pH; mechanism Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
ATP pH 8.5, 30°C, wild-type PFK Escherichia coli
0.055
-
ATP pH 8.5, 30°C, I126A mutant PFK Escherichia coli
0.094
-
ATP pH 8.5, 30°C, R72H mutant PFK Escherichia coli
0.11
-
ATP pH 8.5, 30°C, wild-type PFK Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
containing overproducing plasmid pHE1007
-
Escherichia coli
-
containing overproducing plasmid TG-1
-
Escherichia coli
-
mutant strains R72H and I127A
-
Escherichia coli DF1020
-
containing overproducing plasmid pHE1007
-
Escherichia coli DF1020
-
containing overproducing plasmid TG-1
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Escherichia coli ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 6-phosphate
-
Escherichia coli DF1020 ADP + D-fructose 1,6-bisphosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
fructose 6-phosphate pH 8.5, 30°C, R72H mutant PFK Escherichia coli
57.2
-
fructose 6-phosphate pH 8.5, 30°C, I126A mutant PFK Escherichia coli
111
-
fructose 6-phosphate pH 8.5, 30°C, wild-type PFK Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pH-dependence of kinetic properties Escherichia coli