EC Number   |
General Information |
Reference |
|---|
   3.4.22.B67 | malfunction |
a robust SUMO stress response occurs in response to 1 M sorbitol treatment of a ulp2 deletant strain |
-, 732389 |
| 3.4.22.B67 | malfunction |
deletion of ULP2 promotes the accumulation of poly-SUMO chains and suppresses sgs1DELRA slx5D synthetic lethality and the slx5DELTA sporulation defect. It is speculated that the loss of ULP2 suppresses the toxicity of the sumoylated proteins that accumulate in slx5D-slx8D cells by permitting the extension of poly-SUMO chains on specific target proteins |
715122 |
| 3.4.22.B67 | malfunction |
deletion of ULP2 results in a diverse set of aberrations, but the gene is not essential. In cells lacking Ulp2, specific Smt3-protein conjugates accumulate, and the conjugate pattern is distinct from that observed in a ulp1ts strain, which is defective for a distantly related Smt3-specific protease, Ulp1. The ulp2D mutant exhibits a pleiotropic phenotype that includes temperature-sensitive growth, abnormal cell morphology, decreased plasmid and chromosome stability, and a severe sporulation defect. The mutant is also hypersensitive to DNA damaging agents, hydroxyurea, and benomyl |
716206 |
| 3.4.22.B67 | malfunction |
disruption of Ulp2 causes a range of phenotypic defects, including temperature sensitivity, chromosome instability, and hypersensitivity to DNA and spindle damage. Ulp2-deleted cells died in the presence of DNA damage. In the presence of a DNA double-strand break, ulp2DELTA cells permanently arrested as large-budded cells regardless of whether the DNA DNA double-strand break is repairable or not |
714670 |
| 3.4.22.B67 | malfunction |
in yeast, mutants lacking Ulp2 accumulate high molecular weight SUMO-containing species only in the presence of wild-type Smt3. This is not the case in the smt3 mutants that do not have the critical lysine residues. Ulp2DELTA mutant strains are impaired in the mitotic-specific targeting of the condensin complex to chromatin, in particular rDNA chromatin. In addition, Ulp2 mutants display an increase in premature separation and fail to maintain cohesion at the regions proximal to centromere, which is caused in part by the poor regulation of the SUMO modification status of DNA Topoisomerase II |
714306 |
| 3.4.22.B67 | malfunction |
inactivation of ULP2 in a strain expressing wild-type SUMO-activating enzyme results in slow and temperature-sensitive growth, and accumulation of SUMO conjugates |
716228 |
| 3.4.22.B67 | malfunction |
spontaneous recombination is elevated in ulp2 strains. Ulp2 mutants display a reduced frequency of chromosome rearrangements. ulp2D mutants fail in chromosome segregation after exposure to methyl methanesulfonate |
716666 |
| 3.4.22.B67 | malfunction |
ulp2DELTA mutants do not show persistent Rad53 phosphorylation following DNA damage, suggesting checkpoint signaling has been terminated and no longer asserts an arrest in these cells. Using Cdc14 localization as a cell cycle indicator, it is shown that nearly half of cells lacking Ulp2 can escape a checkpoint-induced metaphase arrest despite their inability to divide again. Moreover, half of permanently arrested ulp2DELTA cells show evidence of an aberrant mitotic spindle, suggesting that Ulp2 is required for proper spindle dynamics during cell cycle resumption following a DNA damage-induced cell cycle arrest |
681925 |
| 3.4.22.B67 | physiological function |
cells lacking Ulp2 display a twofold increase in transcript levels across chromosome I (ChrI) and ChrXII. This is due to the two chromosomes being present at twice their normal copy number. Extra copies of ChrI and ChrXII appear quickly following loss of active Ulp2 and can be eliminated following reintroduction of Ulp2 gene. Increased dosage of two genes on ChrI-CLN3 and CCR4, encoding a G1-phase cyclin and a subunit of the Ccr4-Not deadenylase complex, respectively, suppresses Ulp2-dependent aneuploidy |
753745 |
| 3.4.22.B67 | physiological function |
isozyme Ulp2 controls the dynamic range of small ubiquitin-related modifiers, SUMO, chain lengths by trimming them from the distal ends |
732015 |
