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2'-deoxyadenosine-3'-fluoro-3'-triphosphate + 2'-deoxyadenosine
2'-deoxyadenosine-3'-fluoro-3'-diphosphate + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
?
2'-deoxyadenosine-3'-fluoro-3'-triphosphate + 2'-deoxycytidine
2'-deoxyadenosine-3'-fluoro-3'-diphosphate + 2'-deoxycytidine 5'-phosphate
-
-
-
-
?
2'-deoxyadenosine-3'-fluoroara-3'-triphosphate + 2'-deoxyadenosine
2'-deoxyadenosine-3'-fluoroara-3'-diphosphate + 2'-deoxyadenosine 5'-phosphate
-
low activity
-
-
?
2'-deoxyadenosine-3'-fluoroara-3'-triphosphate + 2'-deoxycytidine
2'-deoxyadenosine-3'-fluoroara-3'-diphosphate + 2'-deoxycytidine 5'-phosphate
-
low activity
-
-
?
2'-deoxyadenosine-3'-triphosphate + 2'-deoxyadenosine
2'-deoxyadenosine-3'-diphosphate + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
?
2'-deoxyadenosine-3'-triphosphate + 2'-deoxycytidine
2'-deoxyadenosine-3'-diphosphate + 2'-deoxycytidine 5'-phosphate
-
-
-
-
?
3'-deoxyadenosine 2'-triphosphate + 2'-deoxyadenosine
3'-deoxyadenosine-2'-diphosphate + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
?
3'-deoxyadenosine 2'-triphosphate + 2'-deoxycytidine
3'-deoxyadenosine-2'-diphosphate + 2'-deoxycytidine 5'-phosphate
-
-
-
-
?
3'-deoxyadenosine-3'-fluoro-2'-triphosphate + 2'-deoxyadenosine
3'-deoxyadenosine-3'-fluoro-2'-diphosphate + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
?
3'-deoxyadenosine-3'-fluoro-2'-triphosphate + 2'-deoxycytidine
3'-deoxyadenosine-3'-fluoro-2'-diphosphate + 2'-deoxycytidine 5'-phosphate
-
-
-
-
?
3'-deoxyadenosine-3'-fluoroxylo-2'-triphosphate + 2'-deoxyadenosine
3'-deoxyadenosine-3'-fluoroxylo-2'-diphosphate + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
?
3'-deoxyadenosine-3'-fluoroxylo-2'-triphosphate + 2'-deoxycytidine
3'-deoxyadenosine-3'-fluoroxylo-2'-diphosphate + 2'-deoxycytidine 5'-phosphate
-
-
-
-
?
ATP + 1-beta-D-arabinofuranosyl cytosine
ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate
ATP + 1-beta-D-arabinofuranosyl uracil
ADP + 1-beta-D-arabinofuranosyl uracil 5'-phosphate
0.3% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide
ADP + 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide 5'-monophosphate
-
trivial name ribavirin
-
?
ATP + 2',2'-difluoro-2'-deoxycytidine
ADP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate
20% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',2'-difluoro-2'-deoxycytidine
ADP + 2',2'-difluoro-2'-deoxycytidine monophosphate
20% relative activity compared to deoxyadenosine
-
-
?
ATP + 2',2'-difluoro-2'-deoxyguanosine
ADP + 2',2'-difluoro-2'-deoxyguanosine 5'-phosphate
24% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',2'-difluoro-2'-deoxyguanosine
ADP + 2',2'-difluoro-2'-deoxyguanosine monophosphate
24% relative activity compared to deoxyadenosine
-
-
?
ATP + 2',2'-difluorodeoxycytidine
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
ATP + 2',3'-dideoxyadenosine
ADP + 2',3'-dideoxyadenosine 5'-monophosphate
1.5% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyadenosine
ADP + 2',3'-dideoxyadenosine monophosphate
1.5% relative activity compared to deoxyadenosine
-
-
?
ATP + 2',3'-dideoxycytidine
ADP + 2',3'-dideoxycytidine 5'-monophosphate
0.1% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyguanosine
ADP + 2',3'-dideoxyguanosine 5'-monophosphate
0.5% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyinoosine
ADP + 2',3'-dideoxyinosine 5'-monophosphate
3.9% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyinosine
ADP + 2',3'-dideoxyinosine 5'-phosphate
3.9% relative activity compared to deoxyadenosine
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
ATP + 2'-deoxyadenosine
ADP + dAMP
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
97% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxyinosine
ADP + 2'-deoxyinosine 5'-phosphate
116% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
0.01% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
0.9% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-fluoro-arabinosyl adenine
ADP + 2'-fluoro-arabinosyl adenine 5'-monophosphate
15% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-fluoro-arabinosyl adenine
ADP + 2'-fluoro-arabinosyl adenine monophosphate
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
111% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine monophosphate
111% relative activity compared to deoxyadenosine
-
-
?
ATP + 9-beta-D-arabinofuranosyl adenine
ADP + 9-beta-D-arabinofuranosyl adenine 5'-monophosphate
77% relative activity compared to deoxyadenosine
-
-
?
ATP + 9-beta-D-arabinofuranosyl adenine
ADP + 9-beta-D-arabinofuranosyl adenine 5'-phosphate
77% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 9-beta-D-arabinofuranosyl guanine
ADP + 9-beta-D-arabinofuranosyl guanine 5'-monophosphate
6% relative activity compared to deoxyadenosine
-
-
?
ATP + 9-beta-D-arabinofuranosyl guanine
ADP + 9-beta-D-arabinofuranosyl guanine 5'-phosphate
6% of the activity with 2'-deoxyadenosine
-
-
?
ATP + adenosine
ADP + adenosine 5'-monophosphate
7.4% of the activity with 2'-deoxyadenosine
-
-
?
ATP + adenosine
ADP + AMP
ATP + arabinosyl-2-fluoroadenine
ADP + arabinosyl-2-fluoroadenine 5'-phosphate
-
-
-
-
?
ATP + arabinosyladenine
ADP + arabinosyladenine 5'-phosphate
-
-
-
-
?
ATP + cytidine
ADP + CMP
-
approx. 18% of activity with deoxyadenosine
-
?
ATP + cytidine
ADP + cytidine 5'-monophosphate
0.1% of the activity with 2'-deoxyadenosine
-
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
ATP + deoxyadenosine
ADP + dAMP
ATP + deoxycytidine
ADP + dCMP
ATP + deoxycytosine
ADP + dCMP
81% relative activity compared to deoxyadenosine
-
-
?
ATP + deoxyguanosine
ADP + dGMP
ATP + deoxyinosine
ADP + dIMP
116% relative activity compared to deoxyadenosine
-
-
?
ATP + deoxyuridine
ADP + dUMP
0.9% relative activity compared to deoxyadenosine
-
-
?
ATP + fludarabine
ADP + fludarabine 5'-phosphate
-
-
-
-
?
ATP + fludarabine
ADP + fludarabine monophosphate
-
-
-
?
ATP + gemcitabine
ADP + ?
-
-
-
?
ATP + guanosine
ADP + GMP
5.5% relative activity compared to deoxyadenosine
-
-
?
ATP + guanosine
ADP + guanosine 5'-monophosphate
5.5% of the activity with 2'-deoxyadenosine
-
-
?
ATP + inosine
ADP + IMP
5.9% relative activity compared to deoxyadenosine
-
-
?
ATP + inosine
ADP + inosine monophosphate
5.9% of the activity with 2'-deoxyadenosine
-
-
?
ATP + penciclovir
ADP + ?
1.4% relative activity compared to deoxyadenosine
-
-
?
ATP + ribavirin
?
-
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
-
-
-
?
ATP + vidarabine
ADP + vidarabine 5'-phosphate
-
-
-
-
?
CTP + 2'-deoxyadenosine
CDP + 2'-deoxyadenosine 5'-phosphate
CTP, 3% of the activity with ATP
-
-
?
CTP + deoxyadenosine
CDP + dAMP
dATP + deoxyadenosine
dADP + dAMP
2% activity compared to ATP
-
-
?
dCTP + deoxyadenosine
dCDP + dAMP
106% activity compared to ATP
-
-
?
dTTP + deoxyadenosine
dTDP + dAMP
GTP + 2'-deoxyadenosine
GDP + 2'-deoxyadenosine 5'-phosphate
GTP + 2'-deoxycytidine
GDP + 2'-deoxycytidine 5'-phosphate
-
-
-
-
?
GTP + deoxyadenosine
GDP + dAMP
triphosphate + 2'-deoxycytidine
diphosphate + 2'-deoxycytidine 5'-phosphate
-
-
-
-
?
TTP + deoxyadenosine
TDP + dAMP
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
UTP, 2% of the activity with ATP
-
-
?
UTP + deoxyadenosine
UDP + dAMP
additional information
?
-
ATP + 1-beta-D-arabinofuranosyl cytosine
ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate
3% relative activity compared to deoxyadenosine
-
-
?
ATP + 1-beta-D-arabinofuranosyl cytosine
ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate
3% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',2'-difluorodeoxycytidine
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
enzyme activates 2',2'-difluorodesoxycytidine in vivo
-
-
?
ATP + 2',2'-difluorodeoxycytidine
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
ir
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + dAMP
-
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + dAMP
-
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + dAMP
-
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
-
ir
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
81% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-fluoro-arabinosyl adenine
ADP + 2'-fluoro-arabinosyl adenine monophosphate
i.e. fludarabine
-
-
?
ATP + 2'-fluoro-arabinosyl adenine
ADP + 2'-fluoro-arabinosyl adenine monophosphate
15% relative activity compared to deoxyadenosine
-
-
?
ATP + adenosine
ADP + AMP
-
-
-
?
ATP + adenosine
ADP + AMP
7.4% relative activity compared to deoxyadenosine
-
-
?
ATP + adenosine
ADP + AMP
-
kinetic data suggest that different catalytic sites of deoxyadenosine kinase or a different enzyme are responsible for deoxyadenosine and adenosine kinase activities
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
-
involved in biosynthesis of nucleoside monophosphates from preformed deoxyribonucleosides
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
-
key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
dGK/dAK plays an essential role in generating the dexyribonucleotide precursors, dGTP and dATP, for DNA metabolism
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
dGK/dAK plays an essential role in generating the dexyribonucleotide precursors, dGTP and dATP, for DNA metabolism
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
-
involved in nucleoside metabolism
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
transfers a phospho group from specific nucleoside 5'-triphosphate donors to 5'-position of deoxyadenosine
-
?
ATP + deoxyadenosine
ADP + dAMP
-
no activity with adenosine and guanosine
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
enzyme has both adenosine kinase and deoxyadenosine kinase activity
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
enzyme bears two separate but interacting active sites for deoxyadenosine and deoxycytidine kinase activity
-
?
ATP + deoxyadenosine
ADP + dAMP
-
enzyme exists in two heterodimeric complexes, complex I: deoxycytidine/deoxyadenosine kinase and complex II: deoxyguanosine/deoxyadenosine kinase
-
?
ATP + deoxyadenosine
ADP + dAMP
-
enzyme exists in two heterodimeric complexes, complex I: deoxycytidine/deoxyadenosine kinase and complex II: deoxyguanosine/deoxyadenosine kinase
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
100% activity
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
-
no activity with cytidine, uridine, guanosine, deoxyguanosine and thymidine
-
?
ATP + deoxyadenosine
ADP + dAMP
-
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
-
approx. 12% of activity with deoxyadenosine
-
?
ATP + deoxycytidine
ADP + dCMP
-
deoxycytidine kinase is a multisubstrate enzyme that also phosphorylates deoxyadenosine, it exists in different conformational states with different substrate kinetic properties
-
?
ATP + deoxycytidine
ADP + dCMP
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
-
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
-
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
-
approx. 40% of activity with deoxyadenosine
-
?
ATP + deoxyguanosine
ADP + dGMP
97% relative activity compared to deoxyadenosine
-
-
?
CTP + deoxyadenosine
CDP + dAMP
-
-
-
?
CTP + deoxyadenosine
CDP + dAMP
-
-
-
-
?
CTP + deoxyadenosine
CDP + dAMP
57% activity compared to ATP
-
-
?
dTTP + deoxyadenosine
dTDP + dAMP
-
75% of activity with ATP
-
?
dTTP + deoxyadenosine
dTDP + dAMP
44% activity compared to ATP
-
-
?
GTP + 2'-deoxyadenosine
GDP + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
?
GTP + 2'-deoxyadenosine
GDP + 2'-deoxyadenosine 5'-phosphate
GTP, 14% of the activity with ATP
-
-
?
GTP + deoxyadenosine
GDP + dAMP
-
-
-
?
GTP + deoxyadenosine
GDP + dAMP
-
10% of activity with ATP
-
?
TTP + deoxyadenosine
TDP + dAMP
-
-
-
?
TTP + deoxyadenosine
TDP + dAMP
-
-
-
-
?
UTP + deoxyadenosine
UDP + dAMP
-
90% of activity with ATP
-
?
UTP + deoxyadenosine
UDP + dAMP
-
-
-
?
UTP + deoxyadenosine
UDP + dAMP
-
-
-
-
?
UTP + deoxyadenosine
UDP + dAMP
22% activity compared to ATP
-
-
?
additional information
?
-
-
substrate specificity, enzyme is specific for deoxyadenosine and deoxycytidine
-
-
?
additional information
?
-
-
cannot efficiently phosphorylate any other native deoxyribonucleoside than deoxyadenosine
-
-
?
additional information
?
-
cannot efficiently phosphorylate any other native deoxyribonucleoside than deoxyadenosine
-
-
?
additional information
?
-
-
enzyme cannot effectively phopshorylate any other native deoxyribonucleodie besides 2'-deoxyadenosine
-
-
?
additional information
?
-
enzyme cannot effectively phopshorylate any other native deoxyribonucleodie besides 2'-deoxyadenosine
-
-
?
additional information
?
-
-
no activity with diphosphate as phosphate donor, no activity with triphosphate and 2'-deoxyadenosine as substrates
-
-
?
additional information
?
-
-
less than 1% activity with dGTP, deoxyuridine, deoxythymidine, 1-beta-D-arabinofuranosyl uracil, 2',3'-dideoxyguanosine, 2',3'-dideoxycytidine, cytidine, acyclovir, ganciclovir, 2'-deoxy-2'-fluoro-1-beta-D-arabinofuranosyl-5-iodouracil, and 5-fluoro-2'-deoxyuridine
-
-
?
additional information
?
-
less than 1% activity with dGTP, deoxyuridine, deoxythymidine, 1-beta-D-arabinofuranosyl uracil, 2',3'-dideoxyguanosine, 2',3'-dideoxycytidine, cytidine, acyclovir, ganciclovir, 2'-deoxy-2'-fluoro-1-beta-D-arabinofuranosyl-5-iodouracil, and 5-fluoro-2'-deoxyuridine
-
-
?
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0.0335
2',2'-difluorodeoxycytidine
ratio kcat/Km is 5100 per s and M
0.192
2',2'-difluorodesoxycytidine
pH 8.0
0.0227 - 0.0332
2'-deoxyadenosine
0.027 - 0.0344
2'-deoxycytidine
0.146
2'-fluoro-arabinosyl adenine
-
0.0028 - 5
deoxyadenosine
0.027 - 0.18
deoxycytidine
0.019 - 0.021
deoxyguanosine
0.0335
gemcitabine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
3.2
ribavirin
-
pH 7.5, 37°C
0.074
Triphosphate
-
pH 7.5, 37°C, with deoxycytidine
additional information
additional information
-
0.0227
2'-deoxyadenosine
-
0.0292
2'-deoxyadenosine
pH 8.0
0.0332
2'-deoxyadenosine
-
0.027
2'-deoxycytidine
-
0.0344
2'-deoxycytidine
pH 8.0
0.007
ATP
-
pH 7.5, 37°C, with deoxycytidine
0.011
ATP
-
pH 7.6, 37°C, recombinant enzyme
0.016
ATP
-
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.06
ATP
-
pH 7.5, 37°C, with deoxyadenosine
0.11
ATP
pH 8.0, 20°C, R79K:dGK mutant dAK
0.11
ATP
ATP in form of MgATP2-
0.15
ATP
pH 8.0, 20°C, R79K mutant dAK
0.15
ATP
ATP in form of MgATP2-
0.44
ATP
pH 8.0, 20°C, recombinant dAK
0.44
ATP
ATP in form of MgATP2-
0.021
CTP
-
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.022
CTP
-
pH 7.6, 37°C, recombinant enzyme
0.0028
deoxyadenosine
pH 8.0, 20°C, recombinant dAK
0.0049
deoxyadenosine
-
pH 7.4, 37°C, CCRF-CEM leukemia cells
0.0075
deoxyadenosine
pH 8.0, 20°C, R79K mutant dAK
0.01
deoxyadenosine
-
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.012
deoxyadenosine
-
pH 7.6, 37°C, recombinant enzyme
0.018
deoxyadenosine
-
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.029
deoxyadenosine
-
pH 7.4, 37°C, erythrocyte
0.0332
deoxyadenosine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
0.5
deoxyadenosine
-
pH 7.5, 37°C
5
deoxyadenosine
-
pH 7.8, 37°C
0.027
deoxycytidine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
0.121
deoxycytidine
-
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.146
deoxycytidine
-
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.18
deoxycytidine
-
pH 7.6, 37°C, recombinant enzyme
0.019
deoxyguanosine
-
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.02
deoxyguanosine
-
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.021
deoxyguanosine
-
pH 7.6, 37°C, recombinant enzyme
0.063
TTP
-
pH 7.6, 37°C, recombinant enzyme
0.077
TTP
-
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.135
UTP
-
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.18
UTP
-
pH 7.6, 37°C, recombinant enzyme
additional information
additional information
-
kinetic study
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
the reaction with triphosphate follows strict Michaelis-Menten kinetics, while ATP exhibits negative cooperativity
-
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