Literature summary for 2.7.1.76 extracted from

Welin, M.; Wang, L.; Eriksson, S.; Eklund, H.
Structure-function analysis of a bacterial deoxyadenosine kinase reveals the basis for substrate specificity (2007), J. Mol. Biol., 366, 1615-1623.

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate, as well as with products 2'-deoxycytidine 5'-phosphate and 2'-deoxycytidine 5'-diphosphate. Both deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate bind to the enzyme in a feedback-inhibitory manner with the deoxyribonucleoside part in the deoxyribonucleoside binding site and the triphosphates in the P-loop. Superposition with human counterparts deoxyguanosine kinase and deoxycytidine kinase. Overall structures are very similar with a few amino acid alterations in the proximity of the active site	Mycoplasma mycoides
sitting drop vapour diffusion method using 24% (w/v) PEG 3350 and 0.2 M KSCN	Mycoplasma mycoides

Crystallization (Comment)

Organism

in complex with deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate, as well as with products 2'-deoxycytidine 5'-phosphate and 2'-deoxycytidine 5'-diphosphate. Both deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate bind to the enzyme in a feedback-inhibitory manner with the deoxyribonucleoside part in the deoxyribonucleoside binding site and the triphosphates in the P-loop. Superposition with human counterparts deoxyguanosine kinase and deoxycytidine kinase. Overall structures are very similar with a few amino acid alterations in the proximity of the active site

Mycoplasma mycoides

sitting drop vapour diffusion method using 24% (w/v) PEG 3350 and 0.2 M KSCN

Mycoplasma mycoides

Inhibitors

Inhibitors	Comment	Organism	Structure
dATP	feedback inhibitor	Mycoplasma mycoides
dCTP	feedback inhibitor	Mycoplasma mycoides

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25000	-	2 * 25000, Superdex 75 column gel filtration	Mycoplasma mycoides
50000	-	Superdex 75 column gel filtration	Mycoplasma mycoides

Organism

Organism	UniProt	Comment	Textmining
Mycoplasma mycoides	-	subspecies Mycoplasma mycoides mycoides	-
Mycoplasma mycoides	Q93IG4	-	-

Purification (Commentary)

Purification (Comment)	Organism
Superdex 75 column gel filtration	Mycoplasma mycoides

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + 1-beta-D-arabinofuranosyl cytosine	3% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate	-	?
ATP + 1-beta-D-arabinofuranosyl cytosine	3% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate	-	?
ATP + 1-beta-D-arabinofuranosyl uracil	0.3% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 1-beta-D-arabinofuranosyl uracil 5'-phosphate	-	?
ATP + 2',2'-difluoro-2'-deoxycytidine	20% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + 2',2'-difluoro-2'-deoxycytidine monophosphate	-	?
ATP + 2',2'-difluoro-2'-deoxycytidine	20% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate	-	?
ATP + 2',2'-difluoro-2'-deoxyguanosine	24% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + 2',2'-difluoro-2'-deoxyguanosine monophosphate	-	?
ATP + 2',2'-difluoro-2'-deoxyguanosine	24% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2',2'-difluoro-2'-deoxyguanosine 5'-phosphate	-	?
ATP + 2',3'-dideoxyadenosine	1.5% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + 2',3'-dideoxyadenosine monophosphate	-	?
ATP + 2',3'-dideoxyadenosine	1.5% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2',3'-dideoxyadenosine 5'-monophosphate	-	?
ATP + 2',3'-dideoxycytidine	0.1% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2',3'-dideoxycytidine 5'-monophosphate	-	?
ATP + 2',3'-dideoxyguanosine	0.5% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2',3'-dideoxyguanosine 5'-monophosphate	-	?
ATP + 2',3'-dideoxyinoosine	3.9% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2',3'-dideoxyinosine 5'-monophosphate	-	?
ATP + 2',3'-dideoxyinosine	3.9% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + 2',3'-dideoxyinosine 5'-phosphate	-	?
ATP + 2'-deoxyadenosine	-	Mycoplasma mycoides	ADP + 2'-deoxyadenosine 5'-phosphate	-	?
ATP + 2'-deoxycytidine	81% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2'-deoxycytidine 5'-phosphate	-	?
ATP + 2'-deoxyguanosine	97% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2'-deoxyguanosine 5'-phosphate	-	?
ATP + 2'-deoxyinosine	116% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2'-deoxyinosine 5'-phosphate	-	?
ATP + 2'-deoxythymidine	0.01% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2'-deoxythymidine 5'-phosphate	-	?
ATP + 2'-deoxyuridine	0.9% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2'-deoxyuridine 5'-phosphate	-	?
ATP + 2'-fluoro-arabinosyl adenine	15% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + 2'-fluoro-arabinosyl adenine monophosphate	-	?
ATP + 2'-fluoro-arabinosyl adenine	15% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2'-fluoro-arabinosyl adenine 5'-monophosphate	-	?
ATP + 2-chloro-2'-deoxyadenosine	111% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + 2-chloro-2'-deoxyadenosine monophosphate	-	?
ATP + 2-chloro-2'-deoxyadenosine	111% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate	-	?
ATP + 9-beta-D-arabinofuranosyl adenine	77% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + 9-beta-D-arabinofuranosyl adenine 5'-monophosphate	-	?
ATP + 9-beta-D-arabinofuranosyl adenine	77% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 9-beta-D-arabinofuranosyl adenine 5'-phosphate	-	?
ATP + 9-beta-D-arabinofuranosyl guanine	6% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + 9-beta-D-arabinofuranosyl guanine 5'-monophosphate	-	?
ATP + 9-beta-D-arabinofuranosyl guanine	6% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + 9-beta-D-arabinofuranosyl guanine 5'-phosphate	-	?
ATP + adenosine	7.4% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + AMP	-	?
ATP + adenosine	7.4% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + adenosine 5'-monophosphate	-	?
ATP + cytidine	0.1% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + cytidine 5'-monophosphate	-	?
ATP + deoxyadenosine	100% activity	Mycoplasma mycoides	ADP + dAMP	-	?
ATP + deoxycytosine	81% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + dCMP	-	?
ATP + deoxyguanosine	97% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + dGMP	-	?
ATP + deoxyinosine	116% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + dIMP	-	?
ATP + deoxyuridine	0.9% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + dUMP	-	?
ATP + guanosine	5.5% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + GMP	-	?
ATP + guanosine	5.5% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + guanosine 5'-monophosphate	-	?
ATP + inosine	5.9% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + IMP	-	?
ATP + inosine	5.9% of the activity with 2'-deoxyadenosine	Mycoplasma mycoides	ADP + inosine monophosphate	-	?
ATP + penciclovir	1.4% relative activity compared to deoxyadenosine	Mycoplasma mycoides	ADP + ?	-	?
CTP + deoxyadenosine	57% activity compared to ATP	Mycoplasma mycoides	CDP + dAMP	-	?
dATP + deoxyadenosine	2% activity compared to ATP	Mycoplasma mycoides	dADP + dAMP	-	?
dCTP + deoxyadenosine	106% activity compared to ATP	Mycoplasma mycoides	dCDP + dAMP	-	?
dTTP + deoxyadenosine	44% activity compared to ATP	Mycoplasma mycoides	dTDP + dAMP	-	?
additional information	less than 1% activity with dGTP, deoxyuridine, deoxythymidine, 1-beta-D-arabinofuranosyl uracil, 2',3'-dideoxyguanosine, 2',3'-dideoxycytidine, cytidine, acyclovir, ganciclovir, 2'-deoxy-2'-fluoro-1-beta-D-arabinofuranosyl-5-iodouracil, and 5-fluoro-2'-deoxyuridine	Mycoplasma mycoides	?	-	?
UTP + deoxyadenosine	22% activity compared to ATP	Mycoplasma mycoides	UDP + dAMP	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 25000, Superdex 75 column gel filtration	Mycoplasma mycoides

Synonyms

Synonyms	Comment	Organism
dAK	-	Mycoplasma mycoides

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mycoplasma mycoides