Information on EC 2.4.1.162 - aldose beta-D-fructosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.162
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RECOMMENDED NAME
GeneOntology No.
aldose beta-D-fructosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-D-aldosyl1 beta-D-fructoside + D-aldose2 = D-aldose1 + alpha-D-aldosyl2 beta-D-fructoside
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
alpha-D-aldosyl-beta-D-fructoside:aldose 1-beta-D-fructosyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9031-67-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain NCIB 11871
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Manually annotated by BRENDA team
NCIMB 11871
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Manually annotated by BRENDA team
previously Aerobacter levanicum
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1',6'-dichlorosucrose + fructose acceptor
?
show the reaction diagram
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1',6'-dichlorosucrose has some donor activity
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?
6,1',6'-trichlorosucrose + fructose acceptor
?
show the reaction diagram
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6, 1',6'-trichlorosucrose has some donor activity
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?
6-chlorosucrose + fructose acceptor
?
show the reaction diagram
6-deoxy-D-glucose + sucrose
6-deoxysucrose + D-glucose
show the reaction diagram
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-
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?
6-deoxysucrose + fructose acceptor
?
show the reaction diagram
alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl-(1->2)-D-fructofuranoside + sucrose
alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranoside + ?
show the reaction diagram
D-glucose 6-benzoate + sucrose
sucrose 6-benzoate + D-glucose
show the reaction diagram
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-
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?
D-sucrose + D-galactose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2,1)-D-6-O-acetyl-galactopyranoside
show the reaction diagram
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?
D-sucrose + D-galactose 6-phosphate
?
show the reaction diagram
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?
D-sucrose + D-gluconic acid
D-glucose + beta-D-fructofuranosyl-(2,1)-D-gluconic acid
show the reaction diagram
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?
fructose donor + fructose acceptor
D-aldose + D-aldosyl beta-D-fructoside
show the reaction diagram
galactosucrose + fructose acceptor
?
show the reaction diagram
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?
glucose 6-acetate + sucrose
sucrose 6-acetate + D-glucose
show the reaction diagram
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?
raffinose + D-xylose
beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
show the reaction diagram
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?
raffinose + fructose acceptor
corresponding D-aldose + D-aldosyl beta-D-fructoside
show the reaction diagram
raffinose + galactose
beta-D-fructofuranosyl-2,1-D-galactopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
show the reaction diagram
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?
raffinose + xylose
beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
show the reaction diagram
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?
stachyose + fructose acceptor
?
show the reaction diagram
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?
stachyose + fructose acceptor
corresponding D-aldose + D-aldosyl beta-D-fructoside
show the reaction diagram
sucrose + 1-thio-D-glucose
?
show the reaction diagram
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?
sucrose + 3-O-methyl alpha-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-alpha-D-glucopyranoside
show the reaction diagram
sucrose + 3-O-methyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2,1)-3-O-methyl-D-glucoside
show the reaction diagram
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?
sucrose + 3-O-methyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-D-glucopyranoside
show the reaction diagram
sucrose + 4-chloro-4-deoxy-D-galactose
D-glucose + beta-D-fructofuranosyl-(2->1)-4-deoxy-4-chloro-D-galactopyranoside
show the reaction diagram
sucrose + 4-chloro-4-deoxy-D-glucose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2->1)-4-chloro-4-deoxy-D-glucopyranoside 6-acetate
show the reaction diagram
sucrose + 4-chloro-D-galactose
?
show the reaction diagram
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?
sucrose + 5-thio-D-glucose
?
show the reaction diagram
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?
sucrose + 6-chloro-6-deoxy-D-glucose
D-glucose + beta-D-fructofuranosyl-(2,1)-6-chloro-6-deoxy-D-glucoside
show the reaction diagram
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?
sucrose + 6-chloro-6-deoxy-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-chloro-6-deoxy-D-glucoside
show the reaction diagram
sucrose + 6-deoxy-D-glucose
6-deoxysucrose + D-glucose
show the reaction diagram
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?
sucrose + 6-deoxy-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-deoxy-D-glucoside
show the reaction diagram
sucrose + 6-O-benzyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-benzyl-D-glucoside
show the reaction diagram
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?
sucrose + 6-O-benzyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-benzyl-D-glucoside
show the reaction diagram
sucrose + 6-O-methyl-D-galactose
D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-galactoside
show the reaction diagram
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?
sucrose + 6-O-methyl-D-galactose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-methyl-D-galactoside
show the reaction diagram
sucrose + 6-O-methyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-glucoside
show the reaction diagram
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?
sucrose + 6-O-methyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)--6-O-methyl-D-glucoside
show the reaction diagram
sucrose + cellobiose
D-glucose + beta-D-fructofuranosyl-(2,1)-cellobioside
show the reaction diagram
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?
sucrose + cellobiose
D-glucose + beta-D-fructofuranosyl-(2->1)-cellobioside
show the reaction diagram
sucrose + D-arabinose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-arabinoside
show the reaction diagram
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?
sucrose + D-arabinose
D-glucose + beta-D-fructofuranosyl-(2->1)-L-arabinoside
show the reaction diagram
sucrose + D-fucose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-fucoside
show the reaction diagram
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?
sucrose + D-galactose
beta-D-fructofuranosyl-2,1-D-galactopyranoside + D-glucose
show the reaction diagram
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?
sucrose + D-galactose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-galactopyranoside
show the reaction diagram
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?
sucrose + D-galactose
D-glucose + beta-D-fructofuranosyl-(2->1)-D-galactopyranoside
show the reaction diagram
sucrose + D-galactose
galactosylfructoside + D-glucose
show the reaction diagram
sucrose + D-galactose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2->1)-D-6-O-acetyl-galactopyranoside
show the reaction diagram
sucrose + D-gluconic acid
D-glucose + beta-D-fructofuranosyl-(2->1)-D-gluconic acid
show the reaction diagram
sucrose + D-glucose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-acetyl-D-glucoside
show the reaction diagram
sucrose + D-glucose 6-acetate
sucrose 6-acetate + D-glucose
show the reaction diagram
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?
sucrose + D-glucose 6-phosphate
D-glucose + beta-D-fructofuranosyl-(2->1)-D-glucopyranoside 6-phosphate
show the reaction diagram
sucrose + D-glucose 6-phosphate
sucrose 6-phosphate + D-glucose
show the reaction diagram
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?
sucrose + D-glycero-D-galactoheptose
D-glucose + ?
show the reaction diagram
sucrose + D-lactose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-lactoside
show the reaction diagram
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?
sucrose + D-lyxose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-lyxoside
show the reaction diagram
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?
sucrose + D-maltose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-maltoside
show the reaction diagram
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?
sucrose + D-mannose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-mannopyranoside
show the reaction diagram
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?
sucrose + D-mannose
D-glucose + beta-D-fructofuranosyl-(2->1)-D-mannopyranoside
show the reaction diagram
sucrose + D-ribose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-riboside
show the reaction diagram
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?
sucrose + D-ribose
D-glucose + beta-D-fructofuranosyl-(2->1)-D-riboside
show the reaction diagram
sucrose + D-xylose
D-glucose + beta-D-fructofuranosyl-(2,1)-alpha-D-xylopyranoside
show the reaction diagram
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?
sucrose + D-xylose
D-glucose + beta-D-fructofuranosyl-(2->1)-alpha-D-xylopyranoside
show the reaction diagram
sucrose + D-xylose
D-glucose + beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside
show the reaction diagram
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?
sucrose + ethanol
D-glucose + 2-O-ethyl-beta-D-fructofuranose
show the reaction diagram
sucrose + ethyl maltol
?
show the reaction diagram
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?
sucrose + fructose acceptor
corresponding D-aldose + D-aldosyl beta-D-fructoside
show the reaction diagram
sucrose + glycerol
?
show the reaction diagram
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?
sucrose + glycerol
D-glucose + ?
show the reaction diagram
sucrose + isomaltose
D-glucose + beta-D-fructofuranosyl-(2,1)-isomaltoside
show the reaction diagram
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?
sucrose + isomaltose
D-glucose + beta-D-fructofuranosyl-(2->1)-isomaltoside
show the reaction diagram
sucrose + L-arabinose
D-glucose + beta-D-fructofuranosyl-(2,1)-L-arabinoside
show the reaction diagram
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?
sucrose + L-arabinose
D-glucose + beta-D-fructofuranosyl-(2->1)-L-arabinoside
show the reaction diagram
sucrose + L-fucose
D-glucose + beta-D-fructofuranosyl-(2->1)-L-fucoside
show the reaction diagram
sucrose + L-rhamnose
D-glucose + beta-D-fructofuranosyl-(2,1)-L-rhamnoside
show the reaction diagram
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?
sucrose + L-rhamnose
D-glucose + beta-D-fructofuranosyl-(2->1)-L-rhamnoside
show the reaction diagram
sucrose + L-sorbose
?
show the reaction diagram
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?
sucrose + lactose
D-glucose + beta-D-fructofuranosyl-(2->1)-lactoside
show the reaction diagram
sucrose + lyxose
D-glucose + beta-D-fructofuranosyl-(2->1)-lyxoside
show the reaction diagram
sucrose + maltohexaose
D-glucose + beta-D-fructofuranosyl-(2,1)-maltohexaoside
show the reaction diagram
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-
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?
sucrose + maltohexaose
D-glucose + beta-D-fructofuranosyl-(2->1)-maltohexaoside
show the reaction diagram
sucrose + maltol
?
show the reaction diagram
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?
sucrose + maltopentaose
D-glucose + beta-D-fructofuranosyl-(2,1)-maltopentaoside
show the reaction diagram
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?
sucrose + maltopentaose
D-glucose + beta-D-fructofuranosyl-(2->1)-maltopentaoside
show the reaction diagram
sucrose + maltose
D-glucose + beta-D-fructofuranosyl-(2->1)-maltoside
show the reaction diagram
sucrose + maltotetraose
D-glucose + beta-D-fructofuranosyl-(2,1)-maltotetraoside
show the reaction diagram
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?
sucrose + maltotriose
D-glucose + beta-D-fructofuranosyl-(2->1)-maltotrioside
show the reaction diagram
sucrose + maltulose
?
show the reaction diagram
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?
sucrose + melibiose
D-glucose + beta-D-fructofuranosyl-(2,1)-mellibioside
show the reaction diagram
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?
sucrose + melibiose
D-glucose + beta-D-fructofuranosyl-(2->1)-melibioside
show the reaction diagram
sucrose + sorbose
D-glucose + ?
show the reaction diagram
sucrose + xylitol
?
show the reaction diagram
sucrose + xylitol
D-glucose + beta-D-fructofuranosyl-(2->1)-xylitol
show the reaction diagram
sucrose + xylose
beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + D-glucose
show the reaction diagram
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-
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?
sucrose 6-acetate + fructose acceptor
?
show the reaction diagram
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?
verbascose + fructose acceptor
?
show the reaction diagram
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?
xylosucrose + fructose acceptor
?
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
fructose donor + fructose acceptor
D-aldose + D-aldosyl beta-D-fructoside
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no requirement for metal ions
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-glucose
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D-mellibiose
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additional information
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p-hydroxymercuribenzoate does not affect fructosyl transferase activity, the fructosyl transferase is completely resistant to endogenous proteases and to trypsin
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Sucrose
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the fructosyl transferase activity in strain NCIB 11871 is induced only by sucrose, other sugars tested as inducers (glucose, fructose, maltose, and cellobiose) are ineffective
additional information
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the fructosyl transferase is unaffected by anionic, cationic, or nonionic detergents such as SDS, cetyltrimethylammonium bromide, and Triton X-100
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
200 - 700
Sucrose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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the purified enzyme is concentrated 33fold and has an activity of 32 nmol/ml/min
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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the enzyme is stable up to 40°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE-52 cellulose column chromatography, and Mono Q column chromatography
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