KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
200 | - |
sucrose | strain NCIB 11871 | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
extracellular | - |
Erwinia sp. | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fructose donor + fructose acceptor | Bacillus subtilis | - |
D-aldose + D-aldosyl beta-D-fructoside | the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides | ? | |
fructose donor + fructose acceptor | Erwinia sp. | - |
D-aldose + D-aldosyl beta-D-fructoside | the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
NCIB 11871, 11872, 11873 | - |
Erwinia sp. | - |
previously Aerobacter levanicum | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-deoxy-D-glucose + sucrose | - |
Bacillus subtilis | 6-deoxysucrose + D-glucose | - |
? | |
alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl-(1->2)-D-fructofuranoside + sucrose | - |
Bacillus subtilis | alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranoside + ? | - |
? | |
alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl-(1->2)-D-fructofuranoside + sucrose | - |
Erwinia sp. | alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranoside + ? | - |
? | |
D-glucose 6-benzoate + sucrose | - |
Bacillus subtilis | sucrose 6-benzoate + D-glucose | - |
? | |
fructose donor + fructose acceptor | - |
Bacillus subtilis | D-aldose + D-aldosyl beta-D-fructoside | the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides | ? | |
fructose donor + fructose acceptor | - |
Erwinia sp. | D-aldose + D-aldosyl beta-D-fructoside | the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides | ? | |
fructose donor + fructose acceptor | the donor must be a beta-fructosyl ring attached to the anomeric carbon of an aldose by an 1, 2 link, acceptors can be monomeric or oligomeric hexoses or pentoses, no acceptors are inosital, 2-deoxyglucose, glucosamine | Bacillus subtilis | D-aldose + D-aldosyl beta-D-fructoside | - |
? | |
fructose donor + fructose acceptor | the donor must be a beta-fructosyl ring attached to the anomeric carbon of an aldose by an 1, 2 link, acceptors can be monomeric or oligomeric hexoses or pentoses, no acceptors are inosital, 2-deoxyglucose, glucosamine | Erwinia sp. | D-aldose + D-aldosyl beta-D-fructoside | - |
? | |
glucose 6-acetate + sucrose | - |
Bacillus subtilis | sucrose 6-acetate + D-glucose | - |
? | |
raffinose + galactose | - |
Bacillus subtilis | beta-D-fructofuranosyl-2,1-D-galactopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside | - |
? | |
raffinose + xylose | - |
Bacillus subtilis | beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside | - |
? | |
sucrose + 3-O-methyl alpha-D-glucose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-alpha-D-glucopyranoside | - |
? | |
sucrose + 3-O-methyl alpha-D-glucose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-alpha-D-glucopyranoside | - |
? | |
sucrose + 3-O-methyl-D-glucose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-D-glucopyranoside | - |
? | |
sucrose + 3-O-methyl-D-glucose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-D-glucopyranoside | - |
? | |
sucrose + 4-chloro-4-deoxy-D-galactose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-4-deoxy-4-chloro-D-galactopyranoside | - |
? | |
sucrose + 4-chloro-4-deoxy-D-galactose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-4-deoxy-4-chloro-D-galactopyranoside | - |
? | |
sucrose + 4-chloro-4-deoxy-D-glucose 6-acetate | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-4-chloro-4-deoxy-D-glucopyranoside 6-acetate | - |
? | |
sucrose + 4-chloro-4-deoxy-D-glucose 6-acetate | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-4-chloro-4-deoxy-D-glucopyranoside 6-acetate | - |
? | |
sucrose + 6-chloro-6-deoxy-D-glucose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-6-chloro-6-deoxy-D-glucoside | - |
? | |
sucrose + 6-chloro-6-deoxy-D-glucose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-6-chloro-6-deoxy-D-glucoside | - |
? | |
sucrose + 6-deoxy-D-glucose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-6-deoxy-D-glucoside | - |
? | |
sucrose + 6-deoxy-D-glucose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-6-deoxy-D-glucoside | - |
? | |
sucrose + 6-O-benzyl-D-glucose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-benzyl-D-glucoside | - |
? | |
sucrose + 6-O-benzyl-D-glucose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-benzyl-D-glucoside | - |
? | |
sucrose + 6-O-methyl-D-galactose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-methyl-D-galactoside | - |
? | |
sucrose + 6-O-methyl-D-galactose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-methyl-D-galactoside | - |
? | |
sucrose + 6-O-methyl-D-glucose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)--6-O-methyl-D-glucoside | - |
? | |
sucrose + 6-O-methyl-D-glucose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)--6-O-methyl-D-glucoside | - |
? | |
sucrose + cellobiose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-cellobioside | - |
? | |
sucrose + cellobiose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-cellobioside | - |
? | |
sucrose + D-arabinose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-D-arabinoside | - |
? | |
sucrose + D-arabinose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-D-arabinoside | - |
? | |
sucrose + D-galactose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-D-galactopyranoside | - |
? | |
sucrose + D-galactose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-D-galactopyranoside | - |
? | |
sucrose + D-galactose | - |
Bacillus subtilis | beta-D-fructofuranosyl-2,1-D-galactopyranoside + D-glucose | - |
? | |
sucrose + D-galactose 6-acetate | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-D-6-O-acetyl-galactopyranoside | - |
? | |
sucrose + D-galactose 6-acetate | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-D-6-O-acetyl-galactopyranoside | - |
? | |
sucrose + D-gluconic acid | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-D-gluconic acid | - |
? | |
sucrose + D-gluconic acid | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-D-gluconic acid | - |
? | |
sucrose + D-glucose 6-acetate | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-acetyl-D-glucoside | - |
? | |
sucrose + D-glucose 6-acetate | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-acetyl-D-glucoside | - |
? | |
sucrose + D-glucose 6-phosphate | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-D-glucopyranoside 6-phosphate | - |
? | |
sucrose + D-glucose 6-phosphate | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-D-glucopyranoside 6-phosphate | - |
? | |
sucrose + D-glycero-D-galactoheptose | - |
Bacillus subtilis | D-glucose + ? | - |
? | |
sucrose + D-glycero-D-galactoheptose | - |
Erwinia sp. | D-glucose + ? | - |
? | |
sucrose + D-mannose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-D-mannopyranoside | - |
? | |
sucrose + D-mannose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-D-mannopyranoside | - |
? | |
sucrose + D-ribose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-D-riboside | - |
? | |
sucrose + D-ribose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-D-riboside | - |
? | |
sucrose + D-xylose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-alpha-D-xylopyranoside | - |
? | |
sucrose + D-xylose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-alpha-D-xylopyranoside | - |
? | |
sucrose + ethanol | - |
Bacillus subtilis | D-glucose + 2-O-ethyl-beta-D-fructofuranose | - |
? | |
sucrose + ethanol | - |
Erwinia sp. | D-glucose + 2-O-ethyl-beta-D-fructofuranose | - |
? | |
sucrose + glycerol | - |
Bacillus subtilis | D-glucose + ? | - |
? | |
sucrose + glycerol | - |
Erwinia sp. | D-glucose + ? | - |
? | |
sucrose + isomaltose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-isomaltoside | - |
? | |
sucrose + isomaltose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-isomaltoside | - |
? | |
sucrose + L-arabinose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-L-arabinoside | - |
? | |
sucrose + L-arabinose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-L-arabinoside | - |
? | |
sucrose + L-fucose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-L-fucoside | - |
? | |
sucrose + L-fucose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-L-fucoside | - |
? | |
sucrose + L-rhamnose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-L-rhamnoside | - |
? | |
sucrose + L-rhamnose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-L-rhamnoside | - |
? | |
sucrose + lactose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-lactoside | - |
? | |
sucrose + lactose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-lactoside | - |
? | |
sucrose + lyxose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-lyxoside | - |
? | |
sucrose + lyxose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-lyxoside | - |
? | |
sucrose + maltohexaose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-maltohexaoside | - |
? | |
sucrose + maltohexaose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-maltohexaoside | - |
? | |
sucrose + maltopentaose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-maltopentaoside | - |
? | |
sucrose + maltopentaose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-maltopentaoside | - |
? | |
sucrose + maltose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-maltoside | - |
? | |
sucrose + maltose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-maltoside | - |
? | |
sucrose + maltotriose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-maltotrioside | - |
? | |
sucrose + maltotriose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-maltotrioside | - |
? | |
sucrose + melibiose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-melibioside | - |
? | |
sucrose + melibiose | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-melibioside | - |
? | |
sucrose + sorbose | - |
Bacillus subtilis | D-glucose + ? | - |
? | |
sucrose + sorbose | - |
Erwinia sp. | D-glucose + ? | - |
? | |
sucrose + xylitol | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2->1)-xylitol | - |
? | |
sucrose + xylitol | - |
Erwinia sp. | D-glucose + beta-D-fructofuranosyl-(2->1)-xylitol | - |
? | |
sucrose + xylose | - |
Bacillus subtilis | beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable at least 20 min | Bacillus subtilis |
45 | - |
stable at least 20 min | Erwinia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | 6 | - |
Bacillus subtilis |