Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the fructosyl transferase is unaffected by anionic, cationic, or nonionic detergents such as SDS, cetyltrimethylammonium bromide, and Triton X-100 | Bacillus subtilis | |
sucrose | the fructosyl transferase activity in strain NCIB 11871 is induced only by sucrose, other sugars tested as inducers (glucose, fructose, maltose, and cellobiose) are ineffective | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | - |
Bacillus subtilis | |
D-mellibiose | - |
Bacillus subtilis | |
additional information | p-hydroxymercuribenzoate does not affect fructosyl transferase activity, the fructosyl transferase is completely resistant to endogenous proteases and to trypsin | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
700 | - |
sucrose | using D-xylose as the acceptor sugar, at pH 5.4 and 30°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for metal ions | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis NCIB 11871 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-52 cellulose column chromatography, and Mono Q column chromatography | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the purified enzyme is concentrated 33fold and has an activity of 32 nmol/ml/min | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1',6'-dichlorosucrose + fructose acceptor | 1',6'-dichlorosucrose has some donor activity | Bacillus subtilis | ? | - |
? | |
6,1',6'-trichlorosucrose + fructose acceptor | 6, 1',6'-trichlorosucrose has some donor activity | Bacillus subtilis | ? | - |
? | |
6,1',6'-trichlorosucrose + fructose acceptor | 6, 1',6'-trichlorosucrose has some donor activity | Bacillus subtilis NCIB 11871 | ? | - |
? | |
6-chlorosucrose + fructose acceptor | - |
Bacillus subtilis | ? | - |
? | |
6-chlorosucrose + fructose acceptor | - |
Bacillus subtilis NCIB 11871 | ? | - |
? | |
6-deoxysucrose + fructose acceptor | - |
Bacillus subtilis | ? | - |
? | |
6-deoxysucrose + fructose acceptor | - |
Bacillus subtilis NCIB 11871 | ? | - |
? | |
D-sucrose + D-galactose 6-acetate | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-D-6-O-acetyl-galactopyranoside | - |
? | |
D-sucrose + D-galactose 6-phosphate | - |
Bacillus subtilis | ? | - |
? | |
D-sucrose + D-gluconic acid | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-D-gluconic acid | - |
? | |
galactosucrose + fructose acceptor | - |
Bacillus subtilis | ? | - |
? | |
additional information | when oligosaccharide acceptors are used, the acceptor activity decreases with increasing size, as in the homologous series maltose, maltotriose, maltotetrose, etc., reactivity also decreases with the size of the molecule in the homologous series sucrose, raffinose, stachyose, and verbascose, inulin and levan, and also levan that is partially hydrolysed with acid, do not act as donors, lactulose, isomaltulose, D-glucose-1-phosphate, methyl beta-D-glucoside, and D-xylulose are unreactive | Bacillus subtilis | ? | - |
? | |
additional information | when oligosaccharide acceptors are used, the acceptor activity decreases with increasing size, as in the homologous series maltose, maltotriose, maltotetrose, etc., reactivity also decreases with the size of the molecule in the homologous series sucrose, raffinose, stachyose, and verbascose, inulin and levan, and also levan that is partially hydrolysed with acid, do not act as donors, lactulose, isomaltulose, D-glucose-1-phosphate, methyl beta-D-glucoside, and D-xylulose are unreactive | Bacillus subtilis NCIB 11871 | ? | - |
? | |
raffinose + D-xylose | - |
Bacillus subtilis | beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside | - |
? | |
stachyose + fructose acceptor | - |
Bacillus subtilis | ? | - |
? | |
sucrose + 1-thio-D-glucose | - |
Bacillus subtilis | ? | - |
? | |
sucrose + 3-O-methyl-D-glucose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-3-O-methyl-D-glucoside | - |
? | |
sucrose + 4-chloro-D-galactose | - |
Bacillus subtilis | ? | - |
? | |
sucrose + 5-thio-D-glucose | - |
Bacillus subtilis | ? | - |
? | |
sucrose + 6-chloro-6-deoxy-D-glucose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-6-chloro-6-deoxy-D-glucoside | - |
? | |
sucrose + 6-deoxy-D-glucose | - |
Bacillus subtilis | 6-deoxysucrose + D-glucose | - |
? | |
sucrose + 6-O-benzyl-D-glucose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-benzyl-D-glucoside | - |
? | |
sucrose + 6-O-methyl-D-galactose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-galactoside | - |
? | |
sucrose + 6-O-methyl-D-glucose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-glucoside | - |
? | |
sucrose + cellobiose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-cellobioside | - |
? | |
sucrose + D-arabinose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-D-arabinoside | - |
? | |
sucrose + D-fucose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-D-fucoside | - |
? | |
sucrose + D-galactose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-D-galactopyranoside | - |
? | |
sucrose + D-glucose 6-acetate | - |
Bacillus subtilis | sucrose 6-acetate + D-glucose | - |
? | |
sucrose + D-glucose 6-phosphate | - |
Bacillus subtilis | sucrose 6-phosphate + D-glucose | - |
? | |
sucrose + D-lactose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-D-lactoside | - |
? | |
sucrose + D-lyxose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-D-lyxoside | - |
? | |
sucrose + D-maltose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-D-maltoside | - |
? | |
sucrose + D-mannose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-D-mannopyranoside | - |
? | |
sucrose + D-ribose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-D-riboside | - |
? | |
sucrose + D-xylose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-alpha-D-xylopyranoside | - |
? | |
sucrose + D-xylose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside | - |
? | |
sucrose + ethyl maltol | - |
Bacillus subtilis | ? | - |
? | |
sucrose + glycerol | - |
Bacillus subtilis | ? | - |
? | |
sucrose + isomaltose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-isomaltoside | - |
? | |
sucrose + L-arabinose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-L-arabinoside | - |
? | |
sucrose + L-rhamnose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-L-rhamnoside | - |
? | |
sucrose + L-sorbose | - |
Bacillus subtilis | ? | - |
? | |
sucrose + maltohexaose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-maltohexaoside | - |
? | |
sucrose + maltol | - |
Bacillus subtilis | ? | - |
? | |
sucrose + maltopentaose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-maltopentaoside | - |
? | |
sucrose + maltotetraose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-maltotetraoside | - |
? | |
sucrose + maltulose | - |
Bacillus subtilis | ? | - |
? | |
sucrose + melibiose | - |
Bacillus subtilis | D-glucose + beta-D-fructofuranosyl-(2,1)-mellibioside | - |
? | |
sucrose + xylitol | - |
Bacillus subtilis | ? | - |
? | |
sucrose + xylitol | - |
Bacillus subtilis NCIB 11871 | ? | - |
? | |
sucrose 6-acetate + fructose acceptor | - |
Bacillus subtilis | ? | - |
? | |
verbascose + fructose acceptor | - |
Bacillus subtilis | ? | - |
? | |
xylosucrose + fructose acceptor | - |
Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-fructofuranoside, acceptor (aldose) fructosyltransferase | - |
Bacillus subtilis |
beta-fructosyl transferase | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the enzyme is stable up to 40°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Bacillus subtilis |