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Literature summary for 2.4.1.162 extracted from

  • Cheetham, P.S.; Hacking, A.J.; Vlitos, M.
    Synthesis of novel disaccharides by a newly isolated fructosyl transferase from Bacillus subtilis (1989), Enzyme Microb. Technol., 11, 212-219.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information the fructosyl transferase is unaffected by anionic, cationic, or nonionic detergents such as SDS, cetyltrimethylammonium bromide, and Triton X-100 Bacillus subtilis
sucrose the fructosyl transferase activity in strain NCIB 11871 is induced only by sucrose, other sugars tested as inducers (glucose, fructose, maltose, and cellobiose) are ineffective Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
D-glucose
-
Bacillus subtilis
D-mellibiose
-
Bacillus subtilis
additional information p-hydroxymercuribenzoate does not affect fructosyl transferase activity, the fructosyl transferase is completely resistant to endogenous proteases and to trypsin Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
700
-
sucrose using D-xylose as the acceptor sugar, at pH 5.4 and 30°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for metal ions Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis NCIB 11871
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-52 cellulose column chromatography, and Mono Q column chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the purified enzyme is concentrated 33fold and has an activity of 32 nmol/ml/min Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1',6'-dichlorosucrose + fructose acceptor 1',6'-dichlorosucrose has some donor activity Bacillus subtilis ?
-
?
6,1',6'-trichlorosucrose + fructose acceptor 6, 1',6'-trichlorosucrose has some donor activity Bacillus subtilis ?
-
?
6,1',6'-trichlorosucrose + fructose acceptor 6, 1',6'-trichlorosucrose has some donor activity Bacillus subtilis NCIB 11871 ?
-
?
6-chlorosucrose + fructose acceptor
-
Bacillus subtilis ?
-
?
6-chlorosucrose + fructose acceptor
-
Bacillus subtilis NCIB 11871 ?
-
?
6-deoxysucrose + fructose acceptor
-
Bacillus subtilis ?
-
?
6-deoxysucrose + fructose acceptor
-
Bacillus subtilis NCIB 11871 ?
-
?
D-sucrose + D-galactose 6-acetate
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-6-O-acetyl-galactopyranoside
-
?
D-sucrose + D-galactose 6-phosphate
-
Bacillus subtilis ?
-
?
D-sucrose + D-gluconic acid
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-gluconic acid
-
?
galactosucrose + fructose acceptor
-
Bacillus subtilis ?
-
?
additional information when oligosaccharide acceptors are used, the acceptor activity decreases with increasing size, as in the homologous series maltose, maltotriose, maltotetrose, etc., reactivity also decreases with the size of the molecule in the homologous series sucrose, raffinose, stachyose, and verbascose, inulin and levan, and also levan that is partially hydrolysed with acid, do not act as donors, lactulose, isomaltulose, D-glucose-1-phosphate, methyl beta-D-glucoside, and D-xylulose are unreactive Bacillus subtilis ?
-
?
additional information when oligosaccharide acceptors are used, the acceptor activity decreases with increasing size, as in the homologous series maltose, maltotriose, maltotetrose, etc., reactivity also decreases with the size of the molecule in the homologous series sucrose, raffinose, stachyose, and verbascose, inulin and levan, and also levan that is partially hydrolysed with acid, do not act as donors, lactulose, isomaltulose, D-glucose-1-phosphate, methyl beta-D-glucoside, and D-xylulose are unreactive Bacillus subtilis NCIB 11871 ?
-
?
raffinose + D-xylose
-
Bacillus subtilis beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
-
?
stachyose + fructose acceptor
-
Bacillus subtilis ?
-
?
sucrose + 1-thio-D-glucose
-
Bacillus subtilis ?
-
?
sucrose + 3-O-methyl-D-glucose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-3-O-methyl-D-glucoside
-
?
sucrose + 4-chloro-D-galactose
-
Bacillus subtilis ?
-
?
sucrose + 5-thio-D-glucose
-
Bacillus subtilis ?
-
?
sucrose + 6-chloro-6-deoxy-D-glucose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-6-chloro-6-deoxy-D-glucoside
-
?
sucrose + 6-deoxy-D-glucose
-
Bacillus subtilis 6-deoxysucrose + D-glucose
-
?
sucrose + 6-O-benzyl-D-glucose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-benzyl-D-glucoside
-
?
sucrose + 6-O-methyl-D-galactose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-galactoside
-
?
sucrose + 6-O-methyl-D-glucose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-glucoside
-
?
sucrose + cellobiose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-cellobioside
-
?
sucrose + D-arabinose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-arabinoside
-
?
sucrose + D-fucose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-fucoside
-
?
sucrose + D-galactose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-galactopyranoside
-
?
sucrose + D-glucose 6-acetate
-
Bacillus subtilis sucrose 6-acetate + D-glucose
-
?
sucrose + D-glucose 6-phosphate
-
Bacillus subtilis sucrose 6-phosphate + D-glucose
-
?
sucrose + D-lactose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-lactoside
-
?
sucrose + D-lyxose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-lyxoside
-
?
sucrose + D-maltose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-maltoside
-
?
sucrose + D-mannose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-mannopyranoside
-
?
sucrose + D-ribose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-riboside
-
?
sucrose + D-xylose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-alpha-D-xylopyranoside
-
?
sucrose + D-xylose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside
-
?
sucrose + ethyl maltol
-
Bacillus subtilis ?
-
?
sucrose + glycerol
-
Bacillus subtilis ?
-
?
sucrose + isomaltose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-isomaltoside
-
?
sucrose + L-arabinose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-L-arabinoside
-
?
sucrose + L-rhamnose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-L-rhamnoside
-
?
sucrose + L-sorbose
-
Bacillus subtilis ?
-
?
sucrose + maltohexaose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-maltohexaoside
-
?
sucrose + maltol
-
Bacillus subtilis ?
-
?
sucrose + maltopentaose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-maltopentaoside
-
?
sucrose + maltotetraose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-maltotetraoside
-
?
sucrose + maltulose
-
Bacillus subtilis ?
-
?
sucrose + melibiose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-mellibioside
-
?
sucrose + xylitol
-
Bacillus subtilis ?
-
?
sucrose + xylitol
-
Bacillus subtilis NCIB 11871 ?
-
?
sucrose 6-acetate + fructose acceptor
-
Bacillus subtilis ?
-
?
verbascose + fructose acceptor
-
Bacillus subtilis ?
-
?
xylosucrose + fructose acceptor
-
Bacillus subtilis ?
-
?

Synonyms

Synonyms Comment Organism
beta-fructofuranoside, acceptor (aldose) fructosyltransferase
-
Bacillus subtilis
beta-fructosyl transferase
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
the enzyme is stable up to 40°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Bacillus subtilis