Information on EC 1.14.16.5 - alkylglycerol monooxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.16.5
-
RECOMMENDED NAME
GeneOntology No.
alkylglycerol monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-O-alkyl-sn-glycerol + tetrahydrobiopterin + O2 = 1-O-(1-hydroxyalkyl)-sn-glycerol + dihydrobiopterin + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydroxylation
-
-
oxidation
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-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
1-alkyl-sn-glycerol,tetrahydrobiopterin:oxygen oxidoreductase
The enzyme cleaves alkylglycerols, but does not cleave alkenylglycerols (plasmalogens). Requires non-heme iron [7], reduced glutathione and phospholipids for full activity. The product spontaneously breaks down to form a fatty aldehyde and glycerol.
CAS REGISTRY NUMBER
COMMENTARY hide
37256-82-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
slug
-
-
Manually annotated by BRENDA team
Meriones sp.
gerbil
-
-
Manually annotated by BRENDA team
no activity in Drosophila melanogaster
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R)-3-hexadecyloxypropan-1,2-diol + 2-amino-5,6,7,8-tetrahydropteridin-4-one + O2
?
show the reaction diagram
-
-
-
-
?
(2R)-3-octadecyloxypropan-1,2-diol + 2-amino-5,6,7,8-tetrahydropteridin-4-one + O2
?
show the reaction diagram
-
-
-
-
?
(2RS,1'R)-[1-3H1]-hexadecyloxypropane-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
only 6.5% release of the tritium after 20 min, reaction is stereospecific for the pro-HS hydrogen
-
-
?
(2RS,1'S)-[1-3H1]-hexadecyloxypropane-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
release of 37% of the tritium after 20 min, reaction is stereospecific for the pro-HS hydrogen
-
-
?
(2S)-3-hexadecyloxypropan-1,2-diol + 2-amino-5,6,7,8-tetrahydropteridin-4-one + O2
?
show the reaction diagram
-
the oxygen transfer to 1'C of the aliphatic side-chain is highly stereospecific
-
-
?
(2S)-3-octadecyloxypropan-1,2-diol + 2-amino-5,6,7,8-tetrahydropteridin-4-one + O2
?
show the reaction diagram
-
-
-
-
?
(RS)-batyl alcohol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
1-alkyl-sn-glycerol + tetrahydrobiopterin + O2
1-hydroxyalkyl-sn-glycerol + dihydrobiopterin + H2O
show the reaction diagram
1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine + tetrahydrobiopterin + O2
glycerophosphocholine + alkylaldehyde + dihydrobiopterin + H2O
show the reaction diagram
-
1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine is lyso-platelet activating factor
-
-
?
1-O-alkyl-sn-glycerol + tetrahydrobiopterin + O2
1-O-(1-hydroxyalkyl)-sn-glycerol + dihydrobiopterin + H2O
show the reaction diagram
-
-
-
-
?
1-O-alkyl-sn-glycerol 3-phosphate + tetrahydrobiopterin + O2
1-O-(1-hydroxyalkyl)-sn-glycerol 3-phosphate + 6,7-[8]-dihydrobiopterin + H2O
show the reaction diagram
1-O-hexadecyl-sn-glycero-3-phosphocholine + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
1-O-pyrenedecyl-glycerol + tetrahydrobiopterin + O2
?
show the reaction diagram
1-O-pyrenedecyl-sn-glycerol + tetrahydrobiopterin + O2
1-hydroxypyrenedecyl-sn-glycerol + dihydrobiopterin + H2O
show the reaction diagram
-
1-O-pyrenedecyl-sn-glycerol and high-performance liquid chromatographic analysis of reaction mixtures with fluorescence detection, overview
further formation of 1-pyrenedecanoic acid
-
?
1-O-pyrenedecyl-sn-glycerol + tetrahydrobiopterin + O2
?
show the reaction diagram
-
activity assay
-
-
?
1-O-pyrenedecyl-sn-glycerol + tetrahydrobiopterin + O2
? + dihydrobiopterin + H2O
show the reaction diagram
-
-
-
-
?
1-O-pyrenedecyl-sn-glycerol + tetrahydrobiopterin + O2
glycerol + pyrenedecanal + 6,7[8H]-dihydrobiopterin + H2O
show the reaction diagram
-
-
-
-
?
1-O-pyrenenonyl-sn-glycerol + tetrahydrobiopterin + O2
?
show the reaction diagram
-
activity assay
-
-
?
2-(1-hexadecyloxy)propan-1,3-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
2-(1-octadecyloxy)ethanol + RS-(cis)-6,7-dimethyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
2-(1-octadecyloxy)ethanol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
2-1'-n-hexadecyloxyethanol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
2-1'-n-octadecyloxyethanol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
2-hexadecyloxy-ethan-1-ol + 5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
2-hexadecyloxy-ethan-1-ol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
2-hexadecyloxyethan-1-ol + 5,6,7,8-tetrahydrofolic acid + O2
?
show the reaction diagram
-
-
-
-
?
2-hexadecyloxyethan-1-ol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
2-hexadecyloxyethan-1-ol + 6R-tetrahydrobiopterin + O2
?
show the reaction diagram
-
-
-
-
?
2-hexadecyloxypropane-1,3-diol + 6-methyl-5,6,7,8-tetrahydropterin
?
show the reaction diagram
-
-
-
-
?
2-hexadecylthioethan-1-ol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
2-octadecyloxyethan-1-ol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
2-octadecylthioethan-ol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
3-(1-octadecylthio)propan-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
3-1'-hexadecyloxy-2-methoxy-propan-1-ol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
3-1'-hexadecyloxy-2-methoxy-propan-2-ol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
3-1'-n-hexadecylthiopropane-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
3-1'-n-octadecylthiopropane-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
3-hexadecyloxy-1-methoxypropan-1-ol + 6-methyl-5,6,7,8-tetrahydropterin
?
show the reaction diagram
-
-
-
-
?
3-hexadecyloxy-2-methoxypropan-1-ol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
3-O-hexadecyl glycerol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
alkylglycerol + tetrahydrobiopterin + O2
glycerol + fatty aldehyde + 6,7[8H]-dihydrobiopterin + H2O
show the reaction diagram
-
-
after rapid decay of the resulting unstable primary product (hemiacetal)
-
?
batyl alcohol + 6R-5,6,7,8-tetrahydrobiopterin + O2
?
show the reaction diagram
-
i.e. RS-3-(1-octadecyloxy)propan-1,2-doiol
-
-
?
batyl alcohol + 6R-tetrahydrobiopterin + O2
?
show the reaction diagram
-
-
-
-
?
batyl alcohol + RS-(cis)-6,7-dimethyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
i.e. RS-3-(1-octadecyloxy)propan-1,2-diol
-
-
?
cis-RS-3-octadec-9'-enyloxypropane-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
hexadecyl glycerol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
hexadecylglycerol + tetrahydrobiopterin + O2
?
show the reaction diagram
hexadecylglycerol + tetrahydrobiopterin + O2
hexadecanal + glycerol + dihydrobiopterin + H2O
show the reaction diagram
lyso-alkyl-glycerol phospholipid + tetrahydrobiopterin + O2
1-O-(1-hydroxyalkyl)-sn-glycerol + 6,7-[8]-dihydrobiopterin + H2O
show the reaction diagram
-
-
-
-
?
lyso-platelet activating factor + tetrahydrobiopterin + O2
?
show the reaction diagram
octadecylglycerol + tetrahydrobiopterin + O2
?
show the reaction diagram
R-3-(1-octadecyloxy)propan-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
R-3-dodecyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
R-3-eicosanyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
R-3-heneicosanyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
R-3-heptadecyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
R-3-hexadecyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
R-3-nonadecyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
R-3-octadecyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
R-3-pentadecyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
R-3-tridecyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
R-3-undecyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-(1-heptyloxy)propan-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin
?
show the reaction diagram
-
-
-
-
?
RS-3-(1-hexadecyloxy)-1-methoxypropan-2-ol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-(1-hexadecyloxy)-2-hydroxypropane-1-phosphocholine + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-(1-hexadecyloxy)propan-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-(1-hexadecyloxy)propan-1-ol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-(1-oleyloxy)propan-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-docosanyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-dodecyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-heptyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-hexyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-nonyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-octyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-pentyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-propyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-3-tetradecyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-cyclohexylmethoxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-hexadecylthiopropane-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
RS-octadecylthiopropane-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
S-3-(1-hexadecyloxy)-2-hydroxypropane-1-phosphocholine + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
S-3-(1-octadecyloxy)propan-1,2-diol + RS-6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
S-3-octadecyloxypropan-1,2-diol + 6-methyl-5,6,7,8-tetrahydropterin + O2
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-alkyl-sn-glycerol + tetrahydrobiopterin + O2
1-hydroxyalkyl-sn-glycerol + dihydrobiopterin + H2O
show the reaction diagram
1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine + tetrahydrobiopterin + O2
glycerophosphocholine + alkylaldehyde + dihydrobiopterin + H2O
show the reaction diagram
-
1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine is lyso-platelet activating factor
-
-
?
1-O-alkyl-sn-glycerol + tetrahydrobiopterin + O2
1-O-(1-hydroxyalkyl)-sn-glycerol + dihydrobiopterin + H2O
show the reaction diagram
-
-
-
-
?
1-O-alkyl-sn-glycerol 3-phosphate + tetrahydrobiopterin + O2
1-O-(1-hydroxyalkyl)-sn-glycerol 3-phosphate + 6,7-[8]-dihydrobiopterin + H2O
show the reaction diagram
lyso-alkyl-glycerol phospholipid + tetrahydrobiopterin + O2
1-O-(1-hydroxyalkyl)-sn-glycerol + 6,7-[8]-dihydrobiopterin + H2O
show the reaction diagram
-
-
-
-
?
lyso-platelet activating factor + tetrahydrobiopterin + O2
?
show the reaction diagram
additional information
?
-
-
catalyses the cleavage of lipid ethers into glycerol and the corresponding aldehyde
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(6R)-5,6,7,8-tetrahydrobiopterin
-
-
(6R)-tetrahydrobiopterin
(6RS)-6,7-dimethyltetrahydropterin
-
94.2% of the activity with 6R-tetrahydrobiopterin
(6RS)-6-hydroxymethyltetrahydropterin
-
16% of the activity with 6R-tetrahydrobiopterin
(6RS)-6-methyltetrahydropterin
-
60.9% of the activity with 6R-tetrahydrobiopterin
(6RS)-tetrahydrodictyopterin
-
44.7% of the activity with 6R-tetrahydrobiopterin
(6RS)-tetrahydroneopterin
-
124% of the activity with 6R-tetrahydrobiopterin
(6S)-tetrahydrobiopterin
-
3.5% of the activity with 6R-tetrahydrobiopterin
(RS)-6,7-dimethyl-5,6,7,8-tetrahydropterin
-
-
(RS)-6-methyl-5,6,7,8-tetrahydropterin
2-amino-5,6,7,8-tetrahydropteridin-4-one
-
-
5,6,7,8-tetrahydrofolic acid
-
-
5,6,7,8-Tetrahydropterin
-
-
NAD+
-
stimulates
NADH
-
stimulates
tetrahydrobiopterin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2Mo7O24
-
-
-
1,10-phenanthroline
2-(1-octadecylthio)ethanol
-
reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin
3-(1-octadecylthio)propan-1,2-diol
-
0.5 mM, 64% inhibition, reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin
8-hydroxyquinoline
8-Hydroxyquinoline-5-sulfonic acid
-
1.0 mM, 13% inhibition, reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin
CHAPS
-
0.2% or 0.5%, activity is reduced by ca. 15% after 30 min
Digitonin
-
-
H2O2
-
catalase protects from inactivation
hexadecan-1,2-diol
-
reaction with RS-batyl alcohol and 6-methyl-5,6,7,8-tetrahydropterin
hexadecyl phosphocholine
-
-
hexadecyl-L-alpha-lyso-phosphatidylcholine
-
competitive
-
hexadecylphosphocholine
-
-
Mega-10
myristoyl L-alpha-lyso-phosphatidylcholine
-
-
N5-methyltetrahydrobiopterin
-
-
octadecan-1-ol
octadecanol
-
competitive
palmitoyl L-alpha-lyso-phosphatidylcholine
-
-
Phenanthroline
-
competitive inhibitor
tetradecanoyl-L-alpha-lyso-phosphatidylcholine
-
competitive
tetradecyl phosphocholine
-
-
tetradecylphosphocholine
-
competitive
Triton X-100
-
6% loss of activity at 0.1% detergent and 59% loss of activity at 0.3% detergent
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
activates, optimal concentration is 1.25 mM
NH4Cl
-
activates
Phospholipids
-
required for expression of full activity
Triton X-100
-
stimulates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0026
(6R)-tetrahydrobiopterin
-
pH 8.5, 37°C
0.66
1-O-Hexadecylglycerol
-
-
0.0089 - 0.011
1-O-pyrenedecyl-sn-glycerol
0.0183
2-hexadecyloxypropane-1,3-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.228
2-hexadecylthioethan-1-ol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.0313
2-octadecyloxyethan-1-ol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.521
2-octadecylthioethan-1-ol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.265
3-hexadecyloxy-1-methoxypropan-1-ol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.138
6-Methyl-5,6,7,8-tetrahydropterin
-
reaction with RS-batyl alcohol
0.0246
6R-5,6,7,8-tetrahydrobiopterin
-
reaction with batyl alcohol
0.0397
cis-RS-3-octadec-9'-enyloxypropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.0474
R-3-dodecyloxypropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.0718
R-3-heptadecyloxypropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.0329
R-3-hexadecyloxypropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.937
R-3-nonadecyloxypropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.0415
R-3-octadecyloxypropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.0397
R-3-pentadecyloxypropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.0427
R-3-tridecyloxypropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.011
RS-2-(1-hexadecyloxy)propan-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.173
RS-3-(1-hexadecyloxy)-2-hydroxypropane-1-phosphocholine
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.0265
RS-3-(1-hexadecyloxy)1-methoxypropan-2-ol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.1
RS-3-(1-hexadecyloxy)propan-1-ol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
51.3
RS-3-heptyloxypropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.012 - 0.66
RS-3-hexadecyloxypropane-1,2-diol
0.0247 - 0.072
RS-3-octadecyloxypropane-1,2-diol
0.025
RS-batyl alcohol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.121
RS-hexadecylthiopropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.428
RS-octadecylthiopropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.137
S-3-(1-hexadecyloxy)-2-hydroxypropane-1-phosphocholine
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.0375
S-3-hexydecyloxypropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.0204
S-3-octadecyloxypropane-1,2-diol
-
with 6-methyl-5,6,7,8-tetrahydropterin as cofactor
0.00183 - 0.0335
tetrahydrobiopterin
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.365
hexadecyl phosphocholine
-
-
0.32
hexadecyl-L-alpha-lyso-phosphatidylcholine
-
-
-
0.37
hexadecylphosphocholine
-
-
0.185 - 1.74
Mega-10
0.326
myristoyl L-alpha-lyso-phosphatidylcholine
-
-
0.765 - 0.77
octadecan-1-ol
0.316
palmitoyl L-alpha-lyso-phosphatidylcholine
-
-
0.33
tetradecanoyl-L-alpha-lyso-phosphatidylcholine
-
-
0.32
tetradecyl phosphocholine
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00139
1,10-phenanthroline
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.00069 - 0.00341
Phenanthroline
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.7
-
formation of glyceryl ether products
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9.5
-
pH 7.5: about 40% of maximal activity, pH 9.5: about 75% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
-
x * 45000, SDS-PAGE
48000
-
x * 48000, SDS-PAGE
60000
-
x * 60000, SDS-PAGE
400000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or -70°C, stable for many months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
6-aminohexyl Sepharose column chromatography, diethylaminoethyl cellulose column chromatography, sucrose density centrifugation, and hexadecylglycerol affinity chromatography
-
microsomes from rat liver are prepared
-
partial, 100fold purification of the native enzyme from liver microsomes
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Chinese hamster ovary cells
-
expressed in CHO cell, expressed in Xenopus laevis oocyte, GFP fusion protein expressed in CHO cell, 6x myc tag version expressed in CHO-K1 cell
-
expressed in CHO-K1 cells
-
expressed in HEK-293 cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
mRNA expression levels decrease with lipopolysaccharide treatments in mouse peritoneal macrophages and RAW264.7 cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C231A
-
the mutant enzyme activity is not significantly different from wild type
C397A
-
the mutant enzyme activity is not significantly different from wild type
D153A
-
the mutant enzyme activity is not significantly different from wild type
D384A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
E137A
-
the mutation leads to an 18fold increase in the Michaelis-Menten constant for tetrahydrobiopterin. The mutant has an approximately 5fold lower affinity for iron
E152A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
E203A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
E212A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
H189A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
H220A
-
the mutant enzyme activity is not significantly different from wild type
Q146A
-
the mutant enzyme activity is not significantly different from wild type
Q166A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
Q191A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
Q197A
-
the mutant has activity of less than 5% of that of the wild type
R396A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
Y174A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
Y230A
-
the mutant enzyme activity is not significantly different from wild type
Y338A
-
the mutant has activity of less than 5% of that of the wild type
D233A
-
inactive
Q197A
-
the mutant retains minimal activity
additional information
-
mutation of each of 8 conserved His residues (to alanine) which are all essential for activity