2.6.1.97: archaeosine synthase
This is an abbreviated version!
For detailed information about archaeosine synthase, go to the full flat file.
Word Map on EC 2.6.1.97
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2.6.1.97
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trna
-
trna-guanine
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nucleoside
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transglycosylase
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7-cyano-7-deazaguanine
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preq0
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arctgts
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crenarchaeota
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euryarchaeota
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nitrile
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7-deazaguanosine
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queuosine
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formamidine
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d-loop
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euryarchaeal
- 2.6.1.97
- trna
- trna-guanine
- nucleoside
- transglycosylase
- 7-cyano-7-deazaguanine
- preq0
- arctgts
- crenarchaeota
- euryarchaeota
- nitrile
- 7-deazaguanosine
-
queuosine
-
formamidine
-
d-loop
-
euryarchaeal
Reaction
Synonyms
ARChaeosine synthase, archaeosine synthase subunit alpha, ArcS, ATP-independent amidinotransferase, lysine transferase, MA_4632, MJ1022, QueF-L, QueF-like, SSO0016, Ta0924, TaArcS, TgtA2, TK2156, TkArcS, tRNA-guanine transglycosylase
ECTree
Advanced search results
Substrates Products
Substrates Products on EC 2.6.1.97 - archaeosine synthase
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REACTION DIAGRAM
L-glutamate + archaeine15 in tRNA
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i.e. G+, which is found only in Archaea at position 15 in the dihydrouridine loop, i.e. D-loop
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
i.e. preQ0-tRNA
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
preQ0-tRNASer is used as substrate
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
preQ0-tRNASer is used as substrate
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
preQ0-tRNASer is used as substrate
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
preQ0-tRNASer is used as substrate
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
reaction of subunit alpha
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
reaction of subunit alpha. G+ is successfully synthesized in recombinant Escherichia coli cells by MaArcTGT and the MaArcS-MaRaSEA complex
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
reaction of subunit alpha
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
reaction of subunit alpha. G+ is successfully synthesized in recombinant Escherichia coli cells by MaArcTGT and the MaArcS-MaRaSEA complex
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
reaction of subunit alpha
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
reaction of subunit alpha. G+ is successfully synthesized in recombinant Escherichia coli cells by MaArcTGT and the MaArcS-MaRaSEA complex
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
reaction of subunit alpha
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
reaction of subunit alpha. G+ is successfully synthesized in recombinant Escherichia coli cells by MaArcTGT and the MaArcS-MaRaSEA complex
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
-
i.e. G+, whcih is found only in Archaea at position 15 in the dihydrouridine loop, i.e. D-loop
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
i.e. preQ0-tRNA
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
reaction of subunit alpha
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
reaction of subunit alpha
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
reaction of subunit alpha
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
-
-
-
?
?
-
recombinant MjTgtA2 converts preQ0-tRNA to G+-tRNA using several nitrogen sources and to do so in an ATP-independent process. MjTgtA2 shows in vitro glutaminase activity
-
-
?
additional information
?
-
-
recombinant MjTgtA2 converts preQ0-tRNA to G+-tRNA using several nitrogen sources and to do so in an ATP-independent process. MjTgtA2 shows in vitro glutaminase activity
-
-
?
additional information
?
-
recombinant MjTgtA2 converts preQ0-tRNA to G+-tRNA using several nitrogen sources and to do so in an ATP-independent process. MjTgtA2 shows in vitro glutaminase activity
-
-
?
additional information
?
-
in the Escherichia coli K12 MG1655 strain, the queF and queC are deleted (DELTAque/DELTAqueF), and the resulting deletion strain is transformed with an expression plasmid containing GAT-queC from Ssulfolobus solfataricus (SSO0016) cloned behind a PBAD promoter. Peaks corresponding to 7-amidino-7-deazaguanosine (archaeosine) and 7-cyano-7-deazaguanine nucleoside are detected in tRNA extracted from the strain expressing SSO0016
-
-
?
additional information
?
-
in the Escherichia coli K12 MG1655 strain, the queF and queC are deleted (DELTAque/DELTAqueF), and the resulting deletion strain is transformed with an expression plasmid containing GAT-queC from Ssulfolobus solfataricus (SSO0016) cloned behind a PBAD promoter. Peaks corresponding to 7-amidino-7-deazaguanosine (archaeosine) and 7-cyano-7-deazaguanine nucleoside are detected in tRNA extracted from the strain expressing SSO0016
-
-
?