Information on EC 2.6.1.97 - archaeosine synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.6.1.97
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RECOMMENDED NAME
GeneOntology No.
archaeosine synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O = L-glutamate + archaeine15 in tRNA
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
archaeosine biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
L-glutamine:7-cyano-7-carbaguanine aminotransferase
In Euryarchaeota the reaction is catalysed by ArcS [1,2]. In Crenarchaeota, which do not have an ArcS homologue, the reaction is catalysed either by a homologue of EC 6.3.4.20, 7-cyano-7-deazaguanine synthase that includes a glutaminase domain (cf. EC 3.5.1.2), or by a homologue of EC 1.7.1.13, preQ1 synthase [2]. The enzyme from the Euryarchaeon Methanocaldococcus jannaschii can also use arginine and ammonium as amino donors.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
overview, gene arcS
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Manually annotated by BRENDA team
gene tgtA2
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Manually annotated by BRENDA team
gene tgtA2
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Manually annotated by BRENDA team
gene mj1022
UniProt
Manually annotated by BRENDA team
no activity in Pyrobaculum calidifontis
strain JCM 11548
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Manually annotated by BRENDA team
no activity in Pyrobaculum islandicum
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Manually annotated by BRENDA team
no activity in Sulfolobus acidocaldarius
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Manually annotated by BRENDA team
gene arcS
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
L-glutamate + archaeine15 in tRNA
show the reaction diagram
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
glutaminase activity assay at
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene tgtA2
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in the Escherichia coli K12 MG1655 strain, the queF and queC are deleted (DELTAque/DELTAqueF), and the resulted deletion strain is transformed with an expression plasmid containing GAT-queC from S. solfataricus (SSO0016) cloned behind a PBAD promoter
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information