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2.6.1.83: LL-diaminopimelate aminotransferase

This is an abbreviated version!
For detailed information about LL-diaminopimelate aminotransferase, go to the full flat file.

Word Map on EC 2.6.1.83

Reaction

LL-2,6-Diaminoheptanedioate
+
2-oxoglutarate
=
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
 +
L-glutamate
 +
H2O

Synonyms

Ald1, aq_273, AtDAP-AT, Ava_1277, Ava_2354, BF2666, CT390, CtDAP-AT, Cthe_0816, DAP, DapI, DapL, Dhaf_1761, diaminopimelate aminotransferase, glr4108, GSU0162, L,L-diaminopimelate aminotransferase, LIC12841, LL-DAP aminotransferase, LL-DAP-AT, LL-DAPAT, LL-diaminopimelate aminotransferase, MA1712, Moth_0889, Msp_0924, MTH52, pc0685, Sfum_0054, sll0480, VsDapL

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.83 LL-diaminopimelate aminotransferase

Cloned

Cloned on EC 2.6.1.83 - LL-diaminopimelate aminotransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA sequence of LL-DAP-AT from Arabidopsis thaliana is optimized for expression in bacteria and cloned in Escherichia coli without its leader sequence but with a C-terminal hexahistidine affinity tag to aid protein purification
cloned in Escherichia coli without its leader sequence but with a C-terminal hexahistidine affinity tag to aid protein purification
DNA chemically synthesized, His-tagged protein expressed in Escherichia coli M15(pREP4) and Escherichia coli BL21(DE3)
expressed in Escherichia coli
-
expressed in Escherichia coli Origami 2(DE3), also used in complementation experiments
-
expression in Escheirchia coli
gene DAP, genotyping
gene dapL, functional recombinant expression of the enzyme in Escherichia coli double mutant strain DELTAdapD/E and complementation, and functional recombinant overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
His-tagged protein (amino acid residue 21-456) expressed in Escherichia coli BL21(DE3)
His-tagged protein expressed in Escherichia coli BL21-CodonPlus-RIPL, also used in functional complementation experiments
-
S- and His-tagged protein expressed in Escherichia coli BL21-CodonPlus-RIPL
-
S- and His-tagged protein expressed in Escherichia coli BL21-CodonPlus-RIPL, also used in complementation experiments