Information on EC 2.6.1.83 - LL-diaminopimelate aminotransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
2.6.1.83
-
RECOMMENDED NAME
GeneOntology No.
LL-diaminopimelate aminotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
LL-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-lysine biosynthesis VI
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lysine metabolism
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Lysine biosynthesis
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Metabolic pathways
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Biosynthesis of secondary metabolites
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
LL-2,6-diaminoheptanedioate:2-oxoglutarate aminotransferase
A pyridoxal-phosphate enzyme. In vivo, the reaction occurs in the opposite direction to that shown above. This is one of the final steps in the lysine-biosynthesis pathway of plants (ranging from mosses to flowering plants). meso-Diaminoheptanedioate, an isomer of LL-2,6-diaminoheptanedioate, and the structurally related compounds lysine and ornithine are not substrates. 2-Oxoglutarate cannot be replaced by oxaloacetate or pyruvate. It is not yet known if the substrate of the biosynthetic reaction is the cyclic or acyclic form of tetrahydropyridine-2,6-dicarboxylate.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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lysine biosynthesis pathway
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O
LL-2,6-diaminoheptanedioate + 2-oxoglutarate
show the reaction diagram
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-
-
-
?
L-2-amino-6-oxopimelate + L-glutamate + H2O
LL-2,6-diaminopimelate + 2-oxoglutarate
show the reaction diagram
L-glutamate + tetrahydrodipicolinate + H2O
LL-diaminopimelate + 2-oxoglutarate
show the reaction diagram
LL-2,6-diaminopimelate + 2-oxoglutarate
2,3,4,5-tetrahydrodipicolinate + L-glutamate + H2O
show the reaction diagram
LL-2,6-diaminopimelate + 2-oxoglutarate
L-2-amino-6-oxopimelate + L-glutamate + H2O
show the reaction diagram
LL-diaminopimelate + 2-oxoglutarate
L-glutamate + tetrahydrodipicolinate + H2O
show the reaction diagram
meso-diaminopimelate + 2-oxoglutarate
L-glutamate + tetrahydrodipicolinate + H2O
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-glutamate + tetrahydrodipicolinate + H2O
LL-diaminopimelate + 2-oxoglutarate
show the reaction diagram
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-
-
-
r
LL-diaminopimelate + 2-oxoglutarate
L-glutamate + tetrahydrodipicolinate + H2O
show the reaction diagram
-
-
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r
additional information
?
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enzyme in the biosynthesis of L-lysine
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2-[(E)-[1-(4-chlorophenyl)-2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene]methyl]-1H-indol-1-yl)acetic acid
-
14% inhibition at 0.01 mM
(2-[(E)-[1-methyl-3-(4-methylphenyl)-2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene]methyl]-1H-indol-1-yl)acetic acid
-
17% inhibition at 0.01 mM
(2E)-2-cyano-3-[5-(dimethylamino)furan-2-yl]-N-(3,4-dimethylphenyl)prop-2-enamide
-
14% inhibition at 0.01 mM
(2Z)-N'-nitro-2-[[5-(3-nitrophenyl)furan-2-yl]methylidene]hydrazinecarboximidamide
-
13% inhibition at 0.01 mM
(3E)-1-[(4-methoxyphenyl)sulfonyl]-3-[4-oxo-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-5-ylidene]-1,3-dihydro-2H-indol-2-one
-
18% inhibition at 0.01 mM
(3E)-1-[(4-methylphenyl)sulfonyl]-3-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)-1,3-dihydro-2H-indol-2-one
-
25% inhibition at 0.01 mM
(3E)-3-[2-(2,4-dimethyl-1,3-thiazol-5-yl)-2-oxoethylidene]-3,4-dihydroquinoxalin-2(1H)-one
-
18% inhibition at 0.01 mM
(3E)-3-[2-oxo-2-(thiophen-2-yl)ethylidene]-3,4-dihydroquinoxalin-2(1H)-one
-
15% inhibition at 0.01 mM
(3Z)-3-(2,4-dimethoxybenzylidene)-5-phenylfuran-2(3H)-one
-
14% inhibition at 0.01 mM
(5E)-5-(1H-indol-2-ylmethylidene)-1-(4-methylphenyl)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
-
16% inhibition at 0.01 mM
(5E)-5-[(1-benzyl-1H-indol-2-yl)methylidene]-1-(2-methoxyphenyl)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
-
20% inhibition at 0.01 mM
(5E)-5-[(1-methyl-1H-indol-2-yl)methylidene]-1-(prop-2-en-1-yl)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
-
35% inhibition at 0.01 mM
(5E)-5-[(1-methyl-1H-indol-2-yl)methylidene]-1-prop-2-en-1-yl-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
-
45% inhibition at 0.01 mM
(5E)-5-[[1-(4-nitrobenzyl)-1H-indol-2-yl]methylidene]-1-(prop-2-en-1-yl)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
-
33% inhibition at 0.01 mM
(5Z)-1-(3-bromophenyl)-5-(furan-2-ylmethylidene)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
-
17% inhibition at 0.01 mM
(5Z)-3-(1,1-dioxidotetrahydrothiophen-3-yl)-5-[[1-phenyl-3-(thiophen-2-yl)-1H-pyrazol-4-yl]methylidene]-2-thioxo-1,3-thiazolidin-4-one
-
13% inhibition at 0.01 mM
(5Z)-5-(3-iodo-4-methoxybenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
29% inhibition at 0.01 mM
(5Z)-5-[(3-methylthiophen-2-yl)methylidene]-3-(1-phenylethyl)-2-thioxo-1,3-thiazolidin-4-one
-
13% inhibition at 0.01 mM
(5Z)-5-[(3-methylthiophen-2-yl)methylidene]-3-(tetrahydrofuran-2-ylmethyl)-2-thioxo-1,3-thiazolidin-4-one
-
14% inhibition at 0.01 mM
(5Z)-5-[(6-bromo-1,3-benzodioxol-5-yl)methylidene]-2-(4-methylphenyl)-1,3-thiazol-4(5H)-one
-
13% inhibition at 0.01 mM
(5Z)-5-[5-(2-chlorobenzyl)-2-hydroxybenzylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
-
13% inhibition at 0.01 mM
(5Z)-5-[[5-(2-bromo-4-nitrophenyl)furan-2-yl]methylidene]-2-imino-1,3-thiazolidin-4-one
-
13% inhibition at 0.01 mM
(5Z)-5-[[5-(3-chlorophenyl)furan-2-yl]methylidene]-1-(4-methoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
20% inhibition at 0.01 mM
(Z)-3-amino-5-(4-methoxybenzylidene)-2-thioxothiazolidin-4-one
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-
(Z)-3-ethyl-5-(2-hydroxybenzylidene)-2-thioxothiazolidin-4-one
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-
(Z)-3-ethyl-5-(4-methoxybenzylidene)-2-thioxothiazolidin-4-one
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-
(Z)-3-ethyl-5-(naphthalen-1-ylmethylene)-2-thioxothiazolidin-4-one
-
-
(Z)-3-ethyl-5-(quinolin-5-ylmethylene)-2-thioxothiazolidin-4-one
-
-
(Z)-5-(2-hydroxybenzylidene)-2-thioxothiazolidin-4-one
-
-
(Z)-5-(4-(dimethylamino)benzylidene)-2-thioxothiazolidin-4-one
-
-
(Z)-5-(4-chlorobenzylidene)-2-thioxothiazolidin-4-one
-
-
(Z)-5-(4-chlorobenzylidene)-3-ethyl-2-thioxothiazolidin-4-one
-
-
(Z)-5-(4-methoxybenzylidene)-2-thioxothiazolidin-4-one
-
-
(Z)-5-benzylidene-3-ethyl-2-thioxothiazolidin-4-one
-
-
(Z)-N -(5-((5-(4-nitrophenyl)furan-2-yl)methylene)-4-oxo-2-thioxothiazolidin-3-yl)acetamide
-
-
(Z)-N-(5-(4-methoxybenzylidene)-4-oxo-2-thioxothiazolidin-3-yl)acetamide
-
-
1,3-dimethyl-5-[[1-(3-nitrophenyl)-1H-pyrrol-2-yl]methylidene]pyrimidine-2,4,6(1H,3H,5H)-trione
-
13% inhibition at 0.01 mM
1-(4-fluorophenyl)-2-[[4-phenyl-5-(3,4,5-trimethoxyphenyl)-4H-1,2,4-triazol-3-yl]sulfanyl]ethanone
-
14% inhibition at 0.01 mM
2-(2-[(E)-[1-(4-fluorophenyl)-2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene]methyl]-1H-indol-1-yl)-N-phenylacetamide
-
18% inhibition at 0.01 mM
2-thioxo-5-[(1,2,5-trimethyl-1H-pyrrol-3-yl)methylidene]dihydropyrimidine-4,6(1H,5H)-dione
-
15% inhibition at 0.01 mM
2-[(2E)-2-(2-oxo-5-phenylfuran-3(2H)-ylidene)hydrazinyl]benzoic acid
-
16% inhibition at 0.01 mM
3-cyclopropyl-N'-[(1E)-1-(4-methylphenyl)ethylidene]-1H-pyrazole-5-carbohydrazide
-
14% inhibition at 0.01 mM
3-[2-[(4-acetylphenyl)amino]-1,3-thiazol-4-yl]-2H-chromen-2-one
-
16% inhibition at 0.01 mM
4-chloro-N-[5-fluoro-2-(hydrazinylcarbonyl)phenyl]benzenesulfonamide
-
-
4-fluoro-N-[5-fluoro-2-(hydrazinylcarbonyl)phenyl]benzenesulfonamide
-
-
4-[(E)-[2-(2-chlorophenyl)-5-oxo-1,3-oxazol-4(5H)-ylidene]methyl]-2-hydroxyphenyl 2-chlorobenzoate
-
30% inhibition at 0.01 mM
5-(4-methylphenyl)-4-[[(E)-(2,4,5-trimethoxyphenyl)methylidene]amino]-4H-1,2,4-triazole-3-thiol
-
18% inhibition at 0.01 mM
5-([1-[2-(3,4-dimethylphenoxy)ethyl]-1H-indol-2-yl]methylidene)pyrimidine-2,4,6(1H,3H,5H)-trione
-
28% inhibition at 0.01 mM
5-[(5-hydroxy-1-phenyl-1H-pyrazol-4-yl)methylidene]-1,3-dimethylpyrimidine-2,4,6(1H,3H,5H)-trione
-
20% inhibition at 0.01 mM
5-[3-[(2-chlorobenzyl)oxy]-4-methoxybenzylidene]-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
-
48% inhibition at 0.01 mM
8-methoxy-3-[(2-methylprop-2-en-1-yl)oxy]-6H-benzo[c]chromen-6-one
-
25% inhibition at 0.01 mM
ethyl (5Z)-5-[[4-(dimethylamino)phenyl]methylidene]-2-[(4-methoxyphenyl)amino]-4-oxo-4,5-dihydrothiophene-3-carboxylate
-
22% inhibition at 0.01 mM
ethyl (5Z)-5-[[4-(dimethylamino)phenyl]methylidene]-2-[(4-nitrophenyl)amino]-4-oxo-4,5-dihydrothiophene-3-carboxylate
-
19% inhibition at 0.01 mM
ethyl 4-(5-[(Z)-[2,4,6-trioxo-1-(prop-2-en-1-yl)tetrahydropyrimidin-5(2H)-ylidene]methyl]furan-2-yl)benzoate
-
16% inhibition at 0.01 mM
N'-[(E)-(2,4-dihydroxyphenyl)methylidene]-5-nitro-3-phenyl-1H-indole-2-carbohydrazide
-
15% inhibition at 0.01 mM
N'-[(Z)-(4-fluorophenyl)methylidene]-3-(naphthalen-2-yl)-1H-pyrazole-5-carbohydrazide
-
14% inhibition at 0.01 mM
N-[(2E,5Z)-5-[[5-(4-nitrophenyl)furan-2-yl]methylidene]-4-oxo-1,3-thiazolidin-2-ylidene]acetamide
-
52% inhibition at 0.01 mM
N-[(5E)-5-[(5-bromofuran-2-yl)methylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetamide
-
13% inhibition at 0.01 mM
N-[(5Z)-5-[(5-bromofuran-2-yl)methylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetamide
-
-
N-[2-(hydrazinylcarbonyl)-4,5-dimethoxyphenyl]benzenesulfonamide
-
-
N-[2-(hydrazinylcarbonyl)-5-(trifluoromethyl)phenyl]benzenesulfonamide
-
-
N-[2-(hydrazinylcarbonyl)-5-methoxyphenyl]benzenesulfonamide
-
-
N-[2-(hydrazinylcarbonyl)-5-methylphenyl]benzenesulfonamide
-
-
N-[2-(hydrazinylcarbonyl)phenyl]benzenesulfonamide
-
-
N-[3-(hydrazinylcarbonyl)naphthalen-2-yl]benzenesulfonamide
N-[3-[(2,5-dimethoxyphenyl)amino]quinoxalin-2-yl]-3-nitrobenzenesulfonamide
-
13% inhibition at 0.01 mM
N-[4,5-difluoro-2-(hydrazinylcarbonyl)phenyl]benzenesulfonamide
-
-
N-[5-chloro-2-(hydrazinylcarbonyl)phenyl]benzenesulfonamide
-
-
N-[5-fluoro-2-(hydrazinylcarbonyl)phenyl]-4-methoxybenzenesulfonamide
-
-
N-[5-fluoro-2-(hydrazinylcarbonyl)phenyl]-4-methylbenzenesulfonamide
-
-
N-[5-fluoro-2-(hydrazinylcarbonyl)phenyl]benzenesulfonamide
-
-
N-[[2-(2-chlorophenyl)-1,3-benzoxazol-5-yl]carbamothioyl]-3-methyl-1-benzofuran-2-carboxamide
-
19% inhibition at 0.01 mM
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.42 - 8.7
2-oxoglutarate
0.038
L-2-amino-6-oxopimelate
-
pH 7.5, 30C
0.9 - 1.9
L-glutamate
0.067
LL-2,6-Diaminopimelate
-
pH 7.5, 30C
0.0828 - 0.3
LL-diaminopimelate
0.1
tetrahydrodipicolinate
pH 7.6, 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.31 - 19
LL-diaminopimelate
1.1
tetrahydrodipicolinate
Chlamydomonas reinhardtii
A8IW39
pH 7.6, 30C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.065
(2-[(E)-[1-(4-chlorophenyl)-2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene]methyl]-1H-indol-1-yl)acetic acid
Arabidopsis thaliana
-
-
0.037
(2-[(E)-[1-methyl-3-(4-methylphenyl)-2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene]methyl]-1H-indol-1-yl)acetic acid
Arabidopsis thaliana
-
-
0.2
(2E)-2-cyano-3-[5-(dimethylamino)furan-2-yl]-N-(3,4-dimethylphenyl)prop-2-enamide
0.194
(3E)-1-[(4-methoxyphenyl)sulfonyl]-3-[4-oxo-3-(prop-2-en-1-yl)-2-thioxo-1,3-thiazolidin-5-ylidene]-1,3-dihydro-2H-indol-2-one
Arabidopsis thaliana
-
-
0.07
(3E)-1-[(4-methylphenyl)sulfonyl]-3-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)-1,3-dihydro-2H-indol-2-one
Arabidopsis thaliana
-
-
0.056
(3E)-3-[2-(2,4-dimethyl-1,3-thiazol-5-yl)-2-oxoethylidene]-3,4-dihydroquinoxalin-2(1H)-one
Arabidopsis thaliana
-
-
0.031
(3E)-3-[2-oxo-2-(thiophen-2-yl)ethylidene]-3,4-dihydroquinoxalin-2(1H)-one
Arabidopsis thaliana
-
-
0.159
(3Z)-3-(2,4-dimethoxybenzylidene)-5-phenylfuran-2(3H)-one
Arabidopsis thaliana
-
-
0.164
(5E)-5-(1H-indol-2-ylmethylidene)-1-(4-methylphenyl)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
Arabidopsis thaliana
-
-
0.062
(5E)-5-[(1-benzyl-1H-indol-2-yl)methylidene]-1-(2-methoxyphenyl)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
Arabidopsis thaliana
-
-
0.075
(5E)-5-[(1-methyl-1H-indol-2-yl)methylidene]-1-(prop-2-en-1-yl)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
Arabidopsis thaliana
-
-
0.033
(5E)-5-[(1-methyl-1H-indol-2-yl)methylidene]-1-prop-2-en-1-yl-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
Arabidopsis thaliana
-
-
0.069
(5E)-5-[[1-(4-nitrobenzyl)-1H-indol-2-yl]methylidene]-1-(prop-2-en-1-yl)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
Arabidopsis thaliana
-
-
0.2
(5Z)-1-(3-bromophenyl)-5-(furan-2-ylmethylidene)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
Arabidopsis thaliana
-
-
0.117
(5Z)-3-(1,1-dioxidotetrahydrothiophen-3-yl)-5-[[1-phenyl-3-(thiophen-2-yl)-1H-pyrazol-4-yl]methylidene]-2-thioxo-1,3-thiazolidin-4-one
Arabidopsis thaliana
-
-
0.18
(5Z)-5-(3-iodo-4-methoxybenzylidene)-2-thioxo-1,3-thiazolidin-4-one
Arabidopsis thaliana
-
-
0.127
(5Z)-5-[(3-methylthiophen-2-yl)methylidene]-3-(1-phenylethyl)-2-thioxo-1,3-thiazolidin-4-one
Arabidopsis thaliana
-
-
0.2
(5Z)-5-[(3-methylthiophen-2-yl)methylidene]-3-(tetrahydrofuran-2-ylmethyl)-2-thioxo-1,3-thiazolidin-4-one
Arabidopsis thaliana
-
IC50 above 0.2 mM
0.13
(5Z)-5-[(6-bromo-1,3-benzodioxol-5-yl)methylidene]-2-(4-methylphenyl)-1,3-thiazol-4(5H)-one
Arabidopsis thaliana
-
-
0.046
(5Z)-5-[5-(2-chlorobenzyl)-2-hydroxybenzylidene]-3-ethyl-2-thioxo-1,3-thiazolidin-4-one
Arabidopsis thaliana
-
-
0.085
(5Z)-5-[[5-(2-bromo-4-nitrophenyl)furan-2-yl]methylidene]-2-imino-1,3-thiazolidin-4-one
Arabidopsis thaliana
-
-
0.042
(5Z)-5-[[5-(3-chlorophenyl)furan-2-yl]methylidene]-1-(4-methoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
Arabidopsis thaliana
-
-
0.155
(Z)-3-amino-5-(4-methoxybenzylidene)-2-thioxothiazolidin-4-one
Arabidopsis thaliana
-
in 100 mM HEPES-KOH, pH 7.6
0.2
(Z)-3-ethyl-5-(2-hydroxybenzylidene)-2-thioxothiazolidin-4-one
0.19
(Z)-3-ethyl-5-(quinolin-5-ylmethylene)-2-thioxothiazolidin-4-one
Arabidopsis thaliana
-
in 100 mM HEPES-KOH, pH 7.6
0.2
(Z)-5-(2-hydroxybenzylidene)-2-thioxothiazolidin-4-one
Arabidopsis thaliana
-
IC50 above 0.2 mM, in 100 mM HEPES-KOH, pH 7.6
0.127
(Z)-5-(4-(dimethylamino)benzylidene)-2-thioxothiazolidin-4-one
Arabidopsis thaliana
-
in 100 mM HEPES-KOH, pH 7.6
0.2
(Z)-5-(4-chlorobenzylidene)-2-thioxothiazolidin-4-one
0.142
(Z)-5-(4-methoxybenzylidene)-2-thioxothiazolidin-4-one
Arabidopsis thaliana
-
in 100 mM HEPES-KOH, pH 7.6
0.2
(Z)-5-benzylidene-3-ethyl-2-thioxothiazolidin-4-one
Arabidopsis thaliana
-
IC50 above 0.2 mM, in 100 mM HEPES-KOH, pH 7.6
0.073
(Z)-N -(5-((5-(4-nitrophenyl)furan-2-yl)methylene)-4-oxo-2-thioxothiazolidin-3-yl)acetamide
Arabidopsis thaliana
-
in 100 mM HEPES-KOH, pH 7.6
0.2
(Z)-N-(5-(4-methoxybenzylidene)-4-oxo-2-thioxothiazolidin-3-yl)acetamide
0.052
2-(2-[(E)-[1-(4-fluorophenyl)-2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene]methyl]-1H-indol-1-yl)-N-phenylacetamide
Arabidopsis thaliana
-
-
0.025
2-thioxo-5-[(1,2,5-trimethyl-1H-pyrrol-3-yl)methylidene]dihydropyrimidine-4,6(1H,5H)-dione
Arabidopsis thaliana
-
-
0.039
2-[(2E)-2-(2-oxo-5-phenylfuran-3(2H)-ylidene)hydrazinyl]benzoic acid
Arabidopsis thaliana
-
-
0.2
3-cyclopropyl-N'-[(1E)-1-(4-methylphenyl)ethylidene]-1H-pyrazole-5-carbohydrazide
0.0053
4-chloro-N-[5-fluoro-2-(hydrazinylcarbonyl)phenyl]benzenesulfonamide
Arabidopsis thaliana
-
pH not specified in the publication, temperature not specified in the publication
0.0075
4-fluoro-N-[5-fluoro-2-(hydrazinylcarbonyl)phenyl]benzenesulfonamide
Arabidopsis thaliana
-
pH not specified in the publication, temperature not specified in the publication
0.081
4-[(E)-[2-(2-chlorophenyl)-5-oxo-1,3-oxazol-4(5H)-ylidene]methyl]-2-hydroxyphenyl 2-chlorobenzoate
Arabidopsis thaliana
-
-
0.2
5-(4-methylphenyl)-4-[[(E)-(2,4,5-trimethoxyphenyl)methylidene]amino]-4H-1,2,4-triazole-3-thiol
Arabidopsis thaliana
-
IC50 above 0.2 mM
0.099
5-([1-[2-(3,4-dimethylphenoxy)ethyl]-1H-indol-2-yl]methylidene)pyrimidine-2,4,6(1H,3H,5H)-trione
Arabidopsis thaliana
-
-
0.045
5-[(5-hydroxy-1-phenyl-1H-pyrazol-4-yl)methylidene]-1,3-dimethylpyrimidine-2,4,6(1H,3H,5H)-trione
Arabidopsis thaliana
-
-
0.121
5-[3-[(2-chlorobenzyl)oxy]-4-methoxybenzylidene]-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
Arabidopsis thaliana
-
-
0.2
8-methoxy-3-[(2-methylprop-2-en-1-yl)oxy]-6H-benzo[c]chromen-6-one
0.141
ethyl (5Z)-5-[[4-(dimethylamino)phenyl]methylidene]-2-[(4-nitrophenyl)amino]-4-oxo-4,5-dihydrothiophene-3-carboxylate
Arabidopsis thaliana
-
-
0.2
ethyl 4-(5-[(Z)-[2,4,6-trioxo-1-(prop-2-en-1-yl)tetrahydropyrimidin-5(2H)-ylidene]methyl]furan-2-yl)benzoate
Arabidopsis thaliana
-
IC50 above 0.2 mM
0.045
N'-[(E)-(2,4-dihydroxyphenyl)methylidene]-5-nitro-3-phenyl-1H-indole-2-carbohydrazide
Arabidopsis thaliana
-
-
0.161
N'-[(Z)-(4-fluorophenyl)methylidene]-3-(naphthalen-2-yl)-1H-pyrazole-5-carbohydrazide
Arabidopsis thaliana
-
-
0.078
N-[(2E,5Z)-5-[[5-(4-nitrophenyl)furan-2-yl]methylidene]-4-oxo-1,3-thiazolidin-2-ylidene]acetamide
Arabidopsis thaliana
-
-
0.041
N-[(5E)-5-[(5-bromofuran-2-yl)methylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetamide
0.0207
N-[2-(hydrazinylcarbonyl)-4,5-dimethoxyphenyl]benzenesulfonamide
Arabidopsis thaliana
-
pH not specified in the publication, temperature not specified in the publication
0.0038
N-[2-(hydrazinylcarbonyl)-5-(trifluoromethyl)phenyl]benzenesulfonamide
Arabidopsis thaliana
-
pH not specified in the publication, temperature not specified in the publication
0.0045
N-[2-(hydrazinylcarbonyl)-5-methoxyphenyl]benzenesulfonamide
Arabidopsis thaliana
-
pH not specified in the publication, temperature not specified in the publication
0.0079
N-[2-(hydrazinylcarbonyl)-5-methylphenyl]benzenesulfonamide
Arabidopsis thaliana
-
pH not specified in the publication, temperature not specified in the publication
0.0132
N-[2-(hydrazinylcarbonyl)phenyl]benzenesulfonamide
Arabidopsis thaliana
-
pH not specified in the publication, temperature not specified in the publication
0.005
N-[3-(hydrazinylcarbonyl)naphthalen-2-yl]benzenesulfonamide
0.153
N-[3-[(2,5-dimethoxyphenyl)amino]quinoxalin-2-yl]-3-nitrobenzenesulfonamide
Arabidopsis thaliana
-
-
0.0059
N-[4,5-difluoro-2-(hydrazinylcarbonyl)phenyl]benzenesulfonamide
Arabidopsis thaliana
-
pH not specified in the publication, temperature not specified in the publication
0.0041
N-[5-chloro-2-(hydrazinylcarbonyl)phenyl]benzenesulfonamide
Arabidopsis thaliana
-
pH not specified in the publication, temperature not specified in the publication
0.0034
N-[5-fluoro-2-(hydrazinylcarbonyl)phenyl]-4-methoxybenzenesulfonamide
Arabidopsis thaliana
-
pH not specified in the publication, temperature not specified in the publication
0.0056
N-[5-fluoro-2-(hydrazinylcarbonyl)phenyl]-4-methylbenzenesulfonamide
Arabidopsis thaliana
-
pH not specified in the publication, temperature not specified in the publication
0.0025
N-[5-fluoro-2-(hydrazinylcarbonyl)phenyl]benzenesulfonamide
Arabidopsis thaliana
-
pH not specified in the publication, temperature not specified in the publication
0.083
N-[[2-(2-chlorophenyl)-1,3-benzoxazol-5-yl]carbamothioyl]-3-methyl-1-benzofuran-2-carboxamide
Arabidopsis thaliana
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.39
-
pH 8.5, 70C
0.68
substrates glutamate and tetrahydrodipicolinate, pH 7.6, 30C
0.7
-
substrate tetrahydrodipicolinate, pH not specified in the publication, temperature not specified in the publication
11.6
substrates LL-diaminopimelate and 2-oxoglutarate, pH 7.6, 30C
12
-
substrate L,L-diaminopimelate, pH not specified in the publication, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6
-
with HEPES/KOH buffer
7.9
-
with Tris/HCl buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
-
about 40% activity at pH 6.0, about 50% activity at pH 10.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47900
-
for the protomer
100200
analytical ultracentrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 51000, SDS-PAGE
homodimer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
sequence contains a 36 amino acid transit peptide for plastidial localization
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; hanging drop vapour diffusion method at room temperature, 1.95 A resolution. The structure of AtDAP-AT is determined using the multiple-wavelength anomalous dispersion method with a seleno-methionine derivative
-
in complex with N-(5'-phosphopyridoxyl)-L-glutamate and N-(5'-phosphopyridoxyl)-LL-diaminopimelate, hanging drop vapor diffusion method, using 45% (w/v) (NH4)2SO4, 0.1 M HEPES pH 7.5, 3% (w/v) PEG400
-
sitting-drop vapor diffusion methodPEG 6000
-
PEG 3350, sitting drop method
sitting-drop vapour-diffusion method, PEG 3350
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
pH 8.5, at least for 30 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cell extract for 10 min at 70C, centrifugation, anion-exchange chromatography
-
immobilized metal ion affinity chromatography
immobilized metal ion affinity chromatography (Ni2+), gel filtration
immobilized metal ion affinity chromatography, gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA sequence of LL-DAP-AT from Arabidopsis thaliana is optimized for expression in bacteria and cloned in Escherichia coli without its leader sequence but with a C-terminal hexahistidine affinity tag to aid protein purification; cloned in Escherichia coli without its leader sequence but with a C-terminal hexahistidine affinity tag to aid protein purification
-
DNA chemically synthesized, His-tagged protein expressed in Escherichia coli M15(pREP4) and Escherichia coli BL21(DE3)
-
expressed in Escherichia coli
-
expressed in Escherichia coli Origami 2(DE3), also used in complementation experiments
-
His-tagged protein (amino acid residue 21-456) expressed in Escherichia coli BL21(DE3)
His-tagged protein expressed in Escherichia coli BL21-CodonPlus-RIPL, also used in functional complementation experiments
-
S- and His-tagged protein expressed in Escherichia coli BL21-CodonPlus-RIPL
-
S- and His-tagged protein expressed in Escherichia coli BL21-CodonPlus-RIPL, also used in complementation experiments
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K270N
-
catalytically inactive mutant
K270Q
-
catalytically inactive mutant
Show AA Sequence (1893 entries)
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