2.6.1.109: 8-amino-3,8-dideoxy-alpha-D-manno-octulosonate transaminase

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For detailed information about 8-amino-3,8-dideoxy-alpha-D-manno-octulosonate transaminase, go to the full flat file.

Reaction

Synonyms

kdnA

ECTree

     2 Transferases

         2.6 Transferring nitrogenous groups

             2.6.1 Transaminases

                2.6.1.109 8-amino-3,8-dideoxy-alpha-D-manno-octulosonate transaminase

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Results	in table
39	AA Sequence
1	Cloned(Commentary)
3	Cofactor
1	Crystallization (Commentary)
10	General Information
4	Natural Substrates/ Products (Substrates)
8	Organism
3	Pathway
4	PDB ID
1	pH Optimum
2	Protein Variants
1	Purification (Commentary)
1	Reaction
6	Reference
10	Substrates and Products (Substrate)
5	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Cloned

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Cloned on EC 2.6.1.109 - 8-amino-3,8-dideoxy-alpha-D-manno-octulosonate transaminase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

gene kdnA, encoded in the Kdo8N biosynthetic cluster, DNA and amino acid sequence determination and analysis, subcloning and expression in Escherichia coli strains EC100D and WBB06 at 37°C, recombinant expression of the gene cluster for 8-amino-3,8-dideoxy-D-manno-octulosonic acid biosynthesis from Shewanella oneidensis in Escherichia coli results in lipid A containing 8-amino-3,8-dideoxy-D-manno-octulosonic acid, and in vitro assays confirm the enzymatic functionality converting 3-deoxy-D-manno-octulosonic acid to 8-amino-3,8-dideoxy-D-manno-octulosonic acid, with incorporation into the Kdo8N-lipid A domain of lipopolysaccharide by a metal-dependent oxidase followed by a glutamate-dependent aminotransferase, recombinant expression of His-tagged enzyme in Escherichia coi strain C41

Shewanella oneidensis

Q8EEB1

734255