2.4.1.B71: flavonol-3-O-glucuronosyltransferase

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For detailed information about flavonol-3-O-glucuronosyltransferase, go to the full flat file.

Reaction

Synonyms

flavonoid 3-O-glucuronosyltransferase, glycosyltransferase 5, UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase, VvGT5

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.B71 flavonol-3-O-glucuronosyltransferase

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Results	in table
2	Cloned(Commentary)
1	Crystallization (Commentary)
3	General Information
1	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
2	Organism
1	pI Value
1	Protein Variants
1	Purification (Commentary)
1	Reaction
2	Reference
3	Source Tissue
5	Substrates and Products (Substrate)
2	Subunits
5	Synonyms
1	Systematic Name

Crystallization

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Crystallization on EC 2.4.1.B71 - flavonol-3-O-glucuronosyltransferase

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purified recombinant His-tagged enzyme, sitting-drop vapour-diffusion method, mixing of 0.001 ml of 0.7 mg/ml protein in 20 mM TrisHCl, pH 8.0, 150 mM NaCl, 1 mM dithiothreitol, with 0.001 ml of reservoir solution containing 0.1 M MESNaOH, pH 6.3, 14% w/v PEG 4000, 1 M NaCl, 0.2 M MgCl2, 5mM UDP-glucose, and equilibration against 0.06 ml reservoir solution, 2 days, 20°C, soaking of crystals in 0.1 M MES-NaOH, pH 6.3, 17% w/v PEG 4000, 1 M NaCl, 0.2 M MgCl2, 20mM UDP-glucose, 2 mM quercetin, 20% ethylene glycol, X-ray diffraction structure determination and analysis at 2.2 A resolution

Vitis vinifera

735385