Literature summary for 2.4.1.B71 extracted from

Mizohata, E.; Okuda, T.; Hatanaka, S.; Nakayama, T.; Horikawa, M.; Nakayama, T.; Ono, E.; Inoue, T.
Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera (2013), Acta Crystallogr. Sect. F, 69, 65-68.

Search for more literature for 2.4.1.B71

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Star (DE3)	Vitis vinifera

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged enzyme, sitting-drop vapour-diffusion method, mixing of 0.001 ml of 0.7 mg/ml protein in 20 mM TrisHCl, pH 8.0, 150 mM NaCl, 1 mM dithiothreitol, with 0.001 ml of reservoir solution containing 0.1 M MESNaOH, pH 6.3, 14% w/v PEG 4000, 1 M NaCl, 0.2 M MgCl2, 5mM UDP-glucose, and equilibration against 0.06 ml reservoir solution, 2 days, 20°C, soaking of crystals in 0.1 M MES-NaOH, pH 6.3, 17% w/v PEG 4000, 1 M NaCl, 0.2 M MgCl2, 20mM UDP-glucose, 2 mM quercetin, 20% ethylene glycol, X-ray diffraction structure determination and analysis at 2.2 A resolution	Vitis vinifera

Crystallization (Comment)

Organism

purified recombinant His-tagged enzyme, sitting-drop vapour-diffusion method, mixing of 0.001 ml of 0.7 mg/ml protein in 20 mM TrisHCl, pH 8.0, 150 mM NaCl, 1 mM dithiothreitol, with 0.001 ml of reservoir solution containing 0.1 M MESNaOH, pH 6.3, 14% w/v PEG 4000, 1 M NaCl, 0.2 M MgCl2, 5mM UDP-glucose, and equilibration against 0.06 ml reservoir solution, 2 days, 20°C, soaking of crystals in 0.1 M MES-NaOH, pH 6.3, 17% w/v PEG 4000, 1 M NaCl, 0.2 M MgCl2, 20mM UDP-glucose, 2 mM quercetin, 20% ethylene glycol, X-ray diffraction structure determination and analysis at 2.2 A resolution

Vitis vinifera

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49700	-	x * 49700, SDS-PAGE	Vitis vinifera

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Vitis vinifera	UDP-sugar-dependent glycosyltransferases use a catalytic mechanism that inverts the anomeric configuration of a transferred sugar	?	-	?
UDP-glucuronate + a flavonol	Vitis vinifera	-	UDP + a flavonol 3-O-beta-D-glucuronoside	-	?

Organism

Organism	UniProt	Comment	Textmining
Vitis vinifera	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration	Vitis vinifera

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	-	Vitis vinifera	-
leaf	-	Vitis vinifera	-
seed	-	Vitis vinifera	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	UDP-sugar-dependent glycosyltransferases use a catalytic mechanism that inverts the anomeric configuration of a transferred sugar	Vitis vinifera	?	-	?
additional information	VvGT5 is a UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase, catalysing 3-O-specific glucuronosylation using UDP-glucuronic acid as the sugar donor	Vitis vinifera	?	-	?
UDP-glucuronate + a flavonol	-	Vitis vinifera	UDP + a flavonol 3-O-beta-D-glucuronoside	-	?

Subunits

Subunits	Comment	Organism
?	x * 49700, SDS-PAGE	Vitis vinifera

Synonyms

Synonyms	Comment	Organism
glycosyltransferase 5	-	Vitis vinifera
UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase	-	Vitis vinifera
VvGT5	-	Vitis vinifera

General Information

General Information	Comment	Organism
physiological function	the enzyme catalyzes the 3-O-specific glucuronosylation of flavonols using UDP-glucuronic acid as a sugar donor to produce flavonol 3-O-glucosides, which are important bioactive phytochemicals	Vitis vinifera