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2.4.1.87: N-acetyllactosaminide 3-alpha-galactosyltransferase

This is an abbreviated version!
For detailed information about N-acetyllactosaminide 3-alpha-galactosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.87

Reaction

UDP-alpha-D-galactose
+
beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R
=
UDP
 +
alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R

Synonyms

alpha(1,3)GT, alpha-1,3-galactosyltransferase, alpha-1,3galactosyltransferase, alpha-D-galactosyltransferase, alpha-galactosyltransferase, alpha1,3-galactosyltransferase, alpha1,3-GalT, alpha1,3galactosyltransferase, alpha1,3GalT, alpha1,3GT, alpha3-galactosyltransferase, alpha3Gal-T, alpha3GalT, alpha3GT, beta-D-galactosyl-N-acetylglucosaminylglycopeptide alpha-1,3-galactosyltransferase, blood group glycosyltransferase, EC 2.4.1.124, EC 2.4.1.151, galactosyltransferase, uridine diphosphogalactose-acetyllactosamine, galactosyltransferase, uridine diphosphogalactose-acetyllactosamine alpha1-->3-, galactosyltransferase, uridine diphosphogalactose-galactosylacetylglucosamine alpha-1,3-, galactosyltransferase, uridine diphosphogalactose-galactosylacetylglucosaminylgalactosylglucosylceramide, GALT, GGTA1, glucosaminylglycopeptide alpha-1,3-galactosyltransferase, GTB, iGb3 synthase, iGb3S, N-acetyllactoseaminide 3-alpha-D-galactosyltransferase, one, three-galactosyltransferase, Otg1p, Otg2p, Otg3p, UDP-D-Gal(1,4)-D-GlcNAc alpha(1,3)-galactosyltransferase, UDP-Gal:N-acetyllactoseaminide alpha(1,3)-galactosyltransferase, UDP-galactose beta galactosyl alpha1,3-galactosyltransferase, UDP-galactose beta-galactosyl alpha-1,3-galactosyltransferase, UDP-galactose-acetyllactosamine alpha-D-galactosyltransferase, UDP-galactose-acetyllactoseamine alpha-D-galactosyltransferase, UDP-galactose:beta-D-galactosyl-beta-1,4-N-acetyl-D-glucosaminyl-glycopeptide alpha-1,3-D-galactosyltransferase, UDP-galactose:N-acetyllactoseaminide 3-alpha-D-galactosyltransferase, UDP-GalN-acetyllactoseaminide alpha-1,3-D-UDP-GalGalbeta1-->4GlcNAC-R alpha1-->3-galactosyltransferase, UDPgalactose:beta-D-galactosyl-beta-1,4-N-acetyl-D-glucosaminyl-glycopeptide alpha-1,3-D-galactosyltransferase, uridine diphosphogalactose-acetyllactosamine alpha1-->3-galactosyltransferase, uridine diphosphogalactose-galactosylacetylglucosaminylgalactosylglucosylceramide galactosyltransferase, WciN, wclR

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.87 N-acetyllactosaminide 3-alpha-galactosyltransferase

Crystallization

Crystallization on EC 2.4.1.87 - N-acetyllactosaminide 3-alpha-galactosyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
5% PEG 6000, 0.1 M Tris-HCl, pH 8.0, 10 mM MnCl2, 10 mM UDP, in complex with substrate p-nitrophenyl-beta-galactoside, 25% glycerol as cryoprotectant
AGGL-mutant in complex with UDP-N-acetylgalactosamine with 20 mM MES-NaOH buffer, pH 6, 10% glycerol, 10 mM MnCl2, 10 mM UDP-N-acetylgalactosamine and reservoir solution (10-15% PEG 6000, 0.1 M Tris-HCl, pH 8, 15-25% MPD), and RAAI-mutant with 0.1 M Tris-HCl, pH 8, 10-50% PEG 4000, 0.2 M sodium acetate, 10 mM MnCl2, 10 mM UDP-galactose with the same reservoir solution, vapor diffusion hanging drop method
apo crystals of alpha3GT are grown by the vapour-diffusion, hanging-drop method. Structure of a complex containing an inhibitory analogue of UDP-galactose, UDP-2F-galactose, in a complex with the Arg365Lys mutant of alpha3GT. The binding of a donor substrate analogue induces conformational changes in both the ligand and the enzyme. Two loops of alpha3GT are stabilized in the complex of which the C-terminal region, in particular, is highly flexible. Structural transitions in this region are connected with donor substrate binding and distortion (ground state destabilization), cleavage of the UDP to galactose bond, formation of a binding site for acceptor substrate and UDP release
crystal structure of the Glu317Gln mutant in complex with UDP-Gal is reported
purified recombinant enzyme mutants with bound UDP-Gal and Mn2+, hanging drop vapour diffusion at 16°C, mixing of 0.001 ml protein solution containing 5 mg/ml mutant E317Q in 20 mM MES-NaOH buffer, pH 6.0, and 10% glycerol, containing 10 mM MnCl2 and 10 mM UDP-Gal with 0.001 ml of reservoir solution containing 10% PEG 6000, 0.1 M Tris-HCl, pH 8.0, and 8% MPD, for mutant D316N and D316E, 0.001 ml of 5 mg/ml protein in in 20 mM MES-NaOH buffer, pH 6.0, with 10% glycerol, 10 mM UDP-Gal, 10 mM MnCl2, and 10 mM N-acetyllactosamine, are mixed with 0.001 ml of a reservoir solution containing 5-10% PEG 6000, 0.1 M Tris-HCl, pH 8.0, and 5-14% MPD, X-ray diffraction structure determination and analysis at 1.77-2.2 A resolution
structure analysis of the enzyme in ternary complex with substrate UDP-2F-Gal, crystal structure fold-type A, PDB ID 2VFZ
vapor diffusion hanging drop method
vapour diffusion hanging drop method
-
structure analysis of the enzyme in ternary complex with substrates UDP and lactose, crystal structure fold-type A, PDB ID 1GWF
-