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Literature summary for 2.4.1.87 extracted from

  • Zhang, Y.; Swaminathan, G.J.; Deshpande, A.; Boix, E.; Natesh, R.; Xie, Z.; Acharya, K.R.; Brew, K.
    Roles of individual enzyme-substrate interactions by alpha-1,3-galactosyltransferase in catalysis and specificity (2003), Biochemistry, 42, 13512-13521.
    View publication on PubMed
Search for more literature for 2.4.1.87

Cloned(Commentary)

Cloned (Comment) Organism
catalytic domain (residues 80-386) is expressed in Escherichia coli Bos taurus

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion hanging drop method Bos taurus

Protein Variants

Protein Variants Comment Organism
E317Q turnover number for transferase reaction is approximately 2400times lower than that of the wild-type enzyme 120fold reduction in hydrolysis of UDP-galactose. Km-value for UDP-galactose is unchanged Bos taurus
H280Q 80fold reduction in catalytic activity Bos taurus
Q247E 50fold reduction in turnover number for transferase reaction, unchanged hydrolase activity, Km-values for UDP-galactose and lactose are similar to the wild-type values Bos taurus
R365K mutation reduces turnover-number for UDP-galactose hydrolysis about 10fold but has no significant effect on the affinity for UDP-galactose Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00069
-
 UDP-galactose 37°C, mutant enzyme E317Q, hydrolase reaction Bos taurus
0.01
-
 UDP-galactose 37°C, mutant enzyme R365K, hydrolase reaction Bos taurus
0.1
-
 UDP-galactose 37°C, wild-type enzyme, hydrolase reaction Bos taurus
0.13
-
 UDP-galactose 37°C, mutant enzyme E317Q, galactosyltransferase reaction Bos taurus
0.15
-
 UDP-galactose 37°C, mutant enzyme Q247E, hydrolase reaction Bos taurus
0.43
-
 UDP-galactose 37°C, wild-type enzyme, galactosyltransferase reaction Bos taurus
0.66
-
 UDP-galactose 37°C, mutant enzyme Q247E, galactosyltransferase reaction Bos taurus
0.72
-
 UDP-galactose 37°C, mutant enzyme R365K, galactosyltransferase reaction Bos taurus
1.02
-
 UDP-galactose 37°C, mutant enzyme H280Q, galactosyltransferase reaction Bos taurus
12.8
-
 lactose 37°C, mutant enzyme R365K, galactosyltransferase reaction Bos taurus
19.9
-
 lactose 37°C, wild-type enzyme, galactosyltransferase reaction Bos taurus
20.2
-
 lactose 37°C, mutant enzyme Q247E, galactosyltransferase reaction Bos taurus
33.6
-
 lactose 37°C, mutant enzyme H280Q, galactosyltransferase reaction Bos taurus
233
-
 lactose 37°C, mutant enzyme E317Q, galactosyltransferase reaction Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P14769
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + H2O
-
 Bos taurus UDP + D-galactose
-
 ?
UDP-galactose + lactose
-
 Bos taurus UDP + alpha-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
alpha3GT
-
 Bos taurus
UDP-galactose beta galactosyl alpha1,3-galactosyltransferase
-
 Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00013
-
 UDP-galactose 37°C, mutant enzyme E317Q, hydrolase reaction Bos taurus
0.0014
-
 UDP-galactose 37°C, mutant enzyme R365K, hydrolase reaction Bos taurus
0.011
-
 UDP-galactose 37°C, mutant enzyme Q247E, hydrolase reaction Bos taurus
0.016
-
 UDP-galactose 37°C, wild-type enzyme, hydrolase reaction Bos taurus
0.14
-
 lactose 37°C, mutant enzyme Q247E, galactosyltransferase reaction Bos taurus
0.14
-
 UDP-galactose 37°C, mutant enzyme Q247E, galactosyltransferase reaction Bos taurus
0.17
-
 lactose 37°C, mutant enzyme R365K, galactosyltransferase reaction Bos taurus
0.17
-
 UDP-galactose 37°C, mutant enzyme R365K, galactosyltransferase reaction Bos taurus
0.77
-
 lactose 37°C, mutant enzyme H280Q, galactosyltransferase reaction Bos taurus
0.77
-
 UDP-galactose 37°C, mutant enzyme H280Q, galactosyltransferase reaction Bos taurus
6.4
-
 lactose 37°C, wild-type enzyme, galactosyltransferase reaction Bos taurus
6.4
-
 UDP-galactose 37°C, wild-type enzyme, galactosyltransferase reaction Bos taurus