2.4.1.49: cellodextrin phosphorylase
This is an abbreviated version!
For detailed information about cellodextrin phosphorylase, go to the full flat file.
Reaction
Synonyms
beta-1,4-oligoglucan:orthophosphate glucosyltransferase, CbpA, Ccel_1439, Ccel_2354, Ccel_3412, CDP, cellodextrin phosphorylase, CepB, phosphorylase, cellodextrin, THA_1941
ECTree
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Application
Application on EC 2.4.1.49 - cellodextrin phosphorylase
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synthesis
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enzyme is a good tool for the synthesis of cellodextrins
synthesis
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synthesis of cellulase inhibitors from reaction of 4-O-beta-D-glucopyranosyl-1-deoxynojirimycin or 6-O-beta-cellobiosyl-1-deoxynojirimycin as acceptors with glucose 1-phosphate as donors
synthesis
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simple procedures for synthesizing various sugar length radioactive cellodextrins (G2G6) are developed using the Clostridium thermocellum cellobiose and cellodextrin phosphorylases from small building blocks of nonradioactive glucose-1-phosphate and radioactive glucose
synthesis
biocatalytic production of cellodextrins with a degree of polymerization. Kinetic and thermodynamic restrictions upon alpha-Glc1-P utilization (200 mM; 45°C, pH 7.0) are effectively overcome by in situ removal of the phosphate released via precipitation with Mg2+. The product's degree of polymerization is controlled by the molar ratio of glucose/alpha-Glc1-P used in the reaction. In optimized conversion, 36 g/l soluble cellodextrins are obtained. By keeping the glucose concentration low, the reaction is shifted completely towards insoluble product formation
synthesis
to increase the reaction rate of cellodextrin phosphorolysis, synthetic enzyme complexes containing CDP and phosphoglucomutase are constructed to convert G1P to glucose 6-phosphate rapidly. The CDP-phosphoglucomutase enzyme complex with the highest enhancement of initial reaction rate is integrated into an in vitro synthetic enzymatic biosystem for generating bioelectricity from cellodextrin, exhibiting a much higher current density (3.35fold) and power density (2.14fold) than its counterpart biosystem containing free CDP and phosphoglucomutase mixture