Information on EC 2.4.1.49 - cellodextrin phosphorylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.49
-
RECOMMENDED NAME
GeneOntology No.
cellodextrin phosphorylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[(1->4)-beta-D-glucosyl]n + phosphate = [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(1->4)-beta-D-glucan:phosphate alpha-D-glucosyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
37277-58-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
32meric beta-D-cellobiosyl(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-cellobioside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-xyloside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
?
64meric beta-D-cellobiosyl(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
alpha-D-glucose 1-phosphate + cellobiose
phosphate + cellotriose
show the reaction diagram
alpha-D-glucose 1-phosphate + cellotetraose
phosphate + cellopentaose
show the reaction diagram
alpha-D-glucose 1-phosphate + cellotriose
phosphate + cellotetraose
show the reaction diagram
beta-D-Glc(1-4)-D-Xyl + alpha-D-glucose 1-phosphate
beta-D-Glc-(1-4)-D-Glc-(1-4)-D-Xyl + phosphate
show the reaction diagram
beta-D-Glc(1-4)-D-Xyl + alpha-D-xylose 1-phosphate
beta-D-Xyl-(1-4)-D-Glc-(1-4)-D-Xyl + phosphate
show the reaction diagram
beta-D-Xyl(1-4)-D-Glc + alpha-D-glucose 1-phosphate
beta-D-Glc-(1-4)-D-Xyl-beta-(1-4)-D-Glc + phosphate
show the reaction diagram
beta-D-Xyl(1-4)-D-Glc + alpha-D-xylose 1-phosphate
beta-D-Xyl-(1-4)-D-Xyl-beta-(1-4)-D-Glc + phosphate
show the reaction diagram
beta-Glc-(1-4)-Glc-beta-(1-4)-Xyl + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Glc-beta-(1-4)-Glc-beta-(1-4)-Xyl + phosphate
show the reaction diagram
-
-
-
-
?
beta-Glc-(1-4)-Xyl-beta-(1-4)-Glc + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Glc-beta-(1-4)-Xyl-beta-(1-4)-Glc + phosphate
show the reaction diagram
-
-
-
-
?
beta-Glc-(1-4)-Xyl-beta-(1-4)-Xyl + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Glc-beta-(1-4)-Xyl-beta-(1-4)-Xyl + phosphate
show the reaction diagram
-
-
-
-
?
beta-Xyl-(1-4)-Glc-beta-(1-4)-Glc + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Xyl-beta-(1-4)-Glc-beta-(1-4)-Glc + phosphate
show the reaction diagram
-
-
-
-
?
beta-Xyl-(1-4)-Glc-beta-(1-4)-Glc + alpha-D-xylose 1-phosphate
beta-Xyl-(1-4)-Xyl-beta-(1-4)-Glc-beta-(1-4)-Glc + phosphate
show the reaction diagram
-
-
-
-
?
beta-Xyl-(1-4)-Glc-beta-(1-4)-Xyl + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Xyl-beta-(1-4)-Glc-beta-(1-4)-Xyl + phosphate
show the reaction diagram
-
-
-
-
?
beta-Xyl-(1-4)-Glc-beta-(1-4)-Xyl + alpha-D-xylose 1-phosphate
beta-Xyl-(1-4)-Xyl-beta-(1-4)-Glc-beta-(1-4)-Xyl + phosphate
show the reaction diagram
-
-
-
-
?
beta-Xyl-(1-4)-Xyl-(1-4)-Glc + alpha-D-xylose 1-phosphate
beta-Xyl-(1-4)-Xyl-beta-(1-4)-Xyl-beta-(1-4)-Glc + phosphate
show the reaction diagram
-
-
-
-
?
beta-Xyl-(1-4)-Xyl-beta-(1-4)-Glc + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Xyl-beta-(1-4)-Xyl-beta-(1-4)-Glc + phosphate
show the reaction diagram
-
-
-
-
?
cellobiose + alpha-D-galactose 1-phosphate
cellotriose + phosphate
show the reaction diagram
-
glucolipid is also a substrate
-
-
?
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
show the reaction diagram
cellobiose + alpha-D-glucosyl-1-fluoride
cellotriose + phosphate
show the reaction diagram
-
-
-
?
cellobiose + alpha-D-xylose 1-phosphate
beta-Xyl-(1-4)-Glc-beta-(1-4)Glc + phosphate
show the reaction diagram
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
-
r
celloheptaose + alpha-D-glucose 1-phosphate
cellooctaose + phosphate
show the reaction diagram
-
weak activities on celloheptaose and insoluble regenerated amorphous cellulose
-
-
r
cellopentaose + phosphate
cellotetraose + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
-
r
cellotetraose + phosphate
cellotriose + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
-
r
cellotriose + alpha-D-glucose 1-phosphate
cellotetraose + phosphate
show the reaction diagram
-
-
-
-
?
hexadecameric beta-D-cellobiosyl(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
hexyl beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
?
methyl beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
?
N,N-bis(beta-D-cellobiosyl)succinamide + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
octameric beta-D-cellobiosyl-(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
octyl beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
?
phosphate + cellohexaose
cellopentaose + glucose 1-phosphate
show the reaction diagram
phosphate + cellopentaose
cellotetraose + glucose 1-phosphate
show the reaction diagram
phosphate + cellotetraose
cellotriose + glucose 1-phosphate
show the reaction diagram
phosphate + cellotriose
cellobiose + glucose 1-phosphate
show the reaction diagram
tetrameric beta-D-cellobiosyl-(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
Tris[2-([([beta-cellobiosyl]methyl)carbonyl]amino)ethyl]amine + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
xylobiose + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Xyl-beta-(1-4)-Xyl + phosphate
show the reaction diagram
-
-
-
-
?
xylobiose + alpha-D-xylose 1-phosphate
xylotriose + phosphate
show the reaction diagram
-
-
-
-
?
xylotriose + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-xylotriose + phosphate
show the reaction diagram
-
-
-
-
?
xylotriose + alpha-D-xylose 1-phosphate
xylotetraose + phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
contains no pyridoxyl 5'-phosphate
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dithiothreitol
-
143fold activation at 10 mM
Sr2+
-
slight activation at 1 mM
additional information
-
no activation with 1 mM and 10 mM concentration of Mg2+, Mn2+, Ca2+, Zn2+, Sn2+, Al3+, Cu2+, Ni2+, Co2+, Fe2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
-
slight inhibition at 1 mM
Cd2+
-
slight inhibition at 1 mM
Cu2+
-
slight inhibition at 1 mM
Hg2+
-
slight inhibition at 1 mM
N-ethylmaleimide
-
26% remaining activity at 10 mM
Zn2+
-
slight inhibition at 1 mM
additional information
-
not affected by pyridoxal 5'-phosphate, adenine nucleotides, sugar phosphates and D-glucose
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Reducing agents
-
e.g. cysteine, dithiothreitol, reduced glutathione, absolute requirement
-
additional information
-
not affected by pyridoxal 5'-phosphate, adenine nucleotides, sugar phosphates and D-glucose
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.64
4-nitrophenyl beta-D-glucoside
pH 6.5, 45°C
31.1
alpha-D-galactose 1-phosphate
-
pH 6.5, 45°C
2.4 - 2.438
alpha-D-glucose 1-phosphate
0.447 - 69
cellobiose
0.29 - 5.24
celloheptaose
0.19 - 0.37
cellohexaose
0.17 - 1
Cellopentaose
0.05 - 1
Cellotetraose
0.04 - 1
cellotriose
4.7
glucose 1-phosphate
-
with cellobiose
0.623
octyl beta-D-glucoside
pH 6.5, 45°C
0.13 - 0.26
phosphate
additional information
additional information
-
kinetics determined with cell extracts of cells grown on different sugar compounds
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
19.6
4-nitrophenyl beta-D-glucoside
Clostridium stercorarium
P77846
pH 6.5, 45°C
2.5
alpha-D-galactose 1-phosphate
Clostridium stercorarium
-
pH 6.5, 45°C
27.4
alpha-D-glucose 1-phosphate
0.12 - 18.1
cellobiose
1.08 - 4.53
celloheptaose
6.1
cellohexaose
Clostridium stercorarium
P77846
-
6.6
Cellopentaose
Clostridium stercorarium
P77846
-
3.2 - 6.9
Cellotetraose
2.7 - 4
cellotriose
14.2
octyl beta-D-glucoside
Clostridium stercorarium
P77846
pH 6.5, 45°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
30.6
4-nitrophenyl beta-D-glucoside
Clostridium stercorarium
P77846
pH 6.5, 45°C
712
0.1
alpha-D-galactose 1-phosphate
Clostridium stercorarium
-
pH 6.5, 45°C
493
11.2
alpha-D-glucose 1-phosphate
107
0.00174 - 40.5
cellobiose
82
0.41 - 7.76
celloheptaose
3004
3.9
Cellotetraose
Ruminiclostridium thermocellum
-
-
590
4.94
cellotriose
Ruminiclostridium thermocellum
-
-
516
22.8
octyl beta-D-glucoside
Clostridium stercorarium
P77846
pH 6.5, 45°C
5336
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.071
substrate cellobiose, pH 5.7, 40°C
2
methyl beta-D-glucoside, pH 6.5, 45°C
4.1
4-nitrophenyl beta-D-xyloside, pH 6.5, 45°C
5.5
-
purified recombinant enzyme
8.7
substrate cellotriose, pH 5.7, 40°C
9.2
purified enzyme, assay at 60°C and pH 6.0
11
hexyl beta-D-glucoside, pH 6.5, 45°C
11.2
octyl beta-D-glucoside, pH 6.5, 45°C
11.33
-
partially purified enzyme
12.3
cellobiose, pH 6.5, 45°C
13.6
4-nitrophenyl beta-D-glucoside, pH 6.5, 45°C
16
4-nitrophenyl beta-D-cellobioside, pH 6.5, 45°C
64.2
-
purified enzyme
additional information
-
hydrolytic and phosphorolytic activities in cell extract from cells grown on different sugars
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.4
reverse phosphorolysis reaction
5.7
phosphorolytic activity
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
half-maximal activity at pH 5.0 and pH 8.0
5.5 - 9
-
10% of maximal activity at pH 5.5, 15% of maximal activity at pH 9.0, Tris-acetate buffer
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
cells grown in continuous culture under cellobiose or cellulose limitation over a 10fold range of dilution rates. The gene displays modest difference in expression with growth rate or substrate type
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
111182
-
x * 111182, amino acid sequence determination
115000
200000
-
gel filtration
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 115000, SDS-PAGE, gel filtration
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6 - 7
4°C, 24 h
719009
6 - 9
-
37°C, 1 h, stable
489245
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
inactivation within 20 h
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
absolute requirement for a reducing agent
-
cysteine, 50 mM, stabilizes
-
DTT, 40 mM, stabilizes
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
O2-sensitive, enzyme is inactive in the absence of cysteine or DTT
-
489243
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-5°C, at least 4 months
-
-5°C, crude enzyme preparation, at least 6 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
immobilized metal ion affinity chromatography
immobilized metal ion affinity chromatography (Ni2+)
recombinant enzyme from Escherichia coli
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis
expressed in Escherichia coli
-
expression in Escherichia coli
-
gene cdp, expression in Escherichia coli
-
His-tagged protein
-
His-tagged protein and His-tagged fusion proteins harboring a cellulose binding module expressed in Escherichia coli BL21
-
His-tagged protein expressed in Escherichia coli BL21(DE3)
His-tagged protein expressed in Escherichia coli BL21-CodonPlus RIL and Escherichia coli XL10 Gold
overexpression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D474N
complete loss of activity, catalytic residue
W168A
half of wild type activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis