2.4.1.26: DNA alpha-glucosyltransferase
This is an abbreviated version!
For detailed information about DNA alpha-glucosyltransferase, go to the full flat file.
Reaction
Synonyms
AGT, glucosyltransferase, uridine diphosphoglucose-deoxyribonucleate alpha-, T2-HMC-alpha-glucosyl transferase, T4-HMC-alpha-glucosyl transferase, T6-HMC-alpha-glucosyl transferase, UDPglucose-DNA alpha-glucosyltransferase, uridine diphosphoglucose-deoxyribonucleate alpha-glucosyltransferase
ECTree
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Engineering
Engineering on EC 2.4.1.26 - DNA alpha-glucosyltransferase
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E22A
Tequatrovirus T4
-
site-directed mutagenesis, 50% activity compared to the wild-type enzyme, in absence of acceptor 70% reduced UDG-glucose turnover compared to the wild-type enzyme
E311A
Tequatrovirus T4
-
site-directed mutagenesis, 1-2% activity compared to the wild-type enzyme, in absence of acceptor no remaining UDG-glucose turnover
M231A
Tequatrovirus T4
-
site-directed mutagenesis, 1-2% activity compared to the wild-type enzyme, in absence of acceptor mutant shows an unaltered UDG-glucose turnover compared to the wild-type enzyme
R236A
Tequatrovirus T4
-
site-directed mutagenesis, 27% activity compared to the wild-type enzyme, in absence of acceptor mutant shows an unaltered UDG-glucose turnover compared to the wild-type enzyme
R256A
Tequatrovirus T4
-
site-directed mutagenesis, activity is similar to the wild-type enzyme, in absence of acceptor mutant shows an unaltered UDG-glucose turnover compared to the wild-type enzyme
S106A
Tequatrovirus T4
-
site-directed mutagenesis, 111% activity compared to the wild-type enzyme, in absence of acceptor mutant shows an unaltered UDG-glucose turnover compared to the wild-type enzyme