2.4.1.248: cycloisomaltooligosaccharide glucanotransferase

This is an abbreviated version!
For detailed information about cycloisomaltooligosaccharide glucanotransferase, go to the full flat file.

Word Map on EC 2.4.1.248

2.4.1.248

dextran

circulans

glycoside

polymerization

cyclization

carbohydrate-binding

isomaltooligosaccharides

paenibacillus

transglucosylation

starch

synthesis

subsite

alpha-1,6-linkage

maltooligosaccharides

isomaltotetraose

transglutaminase

dextranolytic

amylose

hollow-fiber

isomaltose

dextranases

thermoanaerobacter

porous

sugar-binding

disproportionation

multilayer

mutans

Reaction

cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond =

Synonyms

cit, CITase, CITase-598K, CITase-T3040, isocyclomaltooligosaccharide glucanotransferase

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.248 cycloisomaltooligosaccharide glucanotransferase

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Results	in table
3	Activating Compound
75	AA Sequence
7	Application
1	CAS Registry Number
9	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
10	General Information
8	Inhibitors
1	kcat/KM [mM/s]
1	KM Value [mM]
1	Metals/Ions
7	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
73	Organism
11	PDB ID
8	pH Optimum
3	pH Range
8	pH Stability
1	pI Value
1	Posttranslational Modification
18	Protein Variants
5	Purification (Commentary)
1	Reaction
29	Reference
3	Specific Activity [micromol/min/mg]
43	Substrates and Products (Substrate)
6	Subunits
15	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
2	Temperature Range [°C]
8	Temperature Stability [°C]

Systematic Name

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Systematic Name on EC 2.4.1.248 - cycloisomaltooligosaccharide glucanotransferase

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(1-6)-alpha-D-glucan:(1-6)-alpha-D-glucan 6-alpha-D-[1-6alpha-D-glucano]-transferase (cyclizing)

Specific for (1->6)-alpha-D-glucans (dextrans) and, unlike cyclomaltodextrin glucanotransferase (EC 2.4.1.19), without activity towards (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points. The enzyme from Bacillus circulans T-3040 has been shown to form cycloisomalto-oligosaccharides of three sizes (7, 8 and 9 glucose units). It will also catalyse the disproportionation of two isomalto-oligosaccharides molecules to yield a series of isomalto-oligosachharides and the addition of D-glucose to cycloisomalto-oligosaccharides with ring opening to form isomalto-oligosaccharides.