2.4.1.248: cycloisomaltooligosaccharide glucanotransferase
This is an abbreviated version!
For detailed information about cycloisomaltooligosaccharide glucanotransferase, go to the full flat file.
Word Map on EC 2.4.1.248
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2.4.1.248
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dextran
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circulans
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glycoside
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polymerization
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cyclization
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carbohydrate-binding
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isomaltooligosaccharides
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paenibacillus
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transglucosylation
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starch
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synthesis
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subsite
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alpha-1,6-linkage
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maltooligosaccharides
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isomaltotetraose
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transglutaminase
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dextranolytic
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amylose
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hollow-fiber
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isomaltose
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dextranases
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thermoanaerobacter
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porous
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sugar-binding
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disproportionation
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multilayer
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mutans
- 2.4.1.248
- dextran
- circulans
- glycoside
- polymerization
-
cyclization
-
carbohydrate-binding
- isomaltooligosaccharides
- paenibacillus
-
transglucosylation
- starch
- synthesis
-
subsite
-
alpha-1,6-linkage
- maltooligosaccharides
- isomaltotetraose
- transglutaminase
-
dextranolytic
- amylose
-
hollow-fiber
- isomaltose
- dextranases
-
thermoanaerobacter
-
porous
-
sugar-binding
-
disproportionation
-
multilayer
- mutans
Reaction
cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond =
Synonyms
cit, CITase, CITase-598K, CITase-T3040, isocyclomaltooligosaccharide glucanotransferase
ECTree
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Cloned
Cloned on EC 2.4.1.248 - cycloisomaltooligosaccharide glucanotransferase
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expression in Escherichia coli
gene cit, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
overexpression of CITase in Bacillus circulans strain G22-10, and, with the alpha-amylase promoter (PamyQ) and amyQ signal sequence of Bacillus amyloliquefaciens, in a protease-deficient Bacillus subtilis strain 168 as extracellular protein. The Bacillus subtilis host-vector system allows production of cycloisomaltooligosaccharides by direct fermentation of dextran along with high CITase production, which is not possible in Bacillus circulans G22-10 due to growth inhibition by dextran at high concentrations and limited production of CITase, stable expression up to 48 h
the C-terminal region of TtCITase (TtCITase-C, Met740-Phe1559), including a CBM35-like region and the GH family 15 domain. The domain is successfully cloned, expressed, and purified
WP_028992696