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2.4.1.22: lactose synthase

This is an abbreviated version!
For detailed information about lactose synthase, go to the full flat file.

Word Map on EC 2.4.1.22

Reaction

UDP-alpha-D-galactose
+
D-glucose
=
UDP
 +
lactose

Synonyms

alpha-lactalbumin, B4GALT1, beta-1,4-galactosyltransferase, beta-1,4-galactosyltransferase 1, beta-GalT, beta1,4-galactosyltransferase, beta1,4-galactosyltransferase-1, beta1,4-galactosyltransferase1, beta4Gal-T1, c-LacdiNAcS, Gal-T1, GalNAc(beta1-4)GlcNAc synthase, GALT, glactosyltransferase, uridine diphosphogalactose-glucose, GT, HP0826, lacdiNAc, LACS, lactose synthase, lactose synthetase, LALBA, LALBA1, LgtB, More, N-acetyllactosamine synthase, UDP-galactose-glucose galactosyltransferase, UDPgalactose-glucose galactosyltransferase, UGT72B1

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.22 lactose synthase

Engineering

Engineering on EC 2.4.1.22 - lactose synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C342T
-
determination and analysis of the closed conformation crystal structure of enzyme mutant in complex with UDP-galactose
additional information
carbohydrate characterization of an antibody (Lo-BM2) expressed in Nicotiana tabacum strain BY-2 cells shows that the purified Lo-BM2 displays N-glycan homogeneity with a high proportion (about 70%) of the complex GnGnXF glycoform. The stable co-expression of a human beta-1,4-galactosyltransferase targeted to different Golgi subcompartments alters Lo-BM2N-glycosylation and results in the production of an antibody that exhibits either hybrid structures containing a low abundance of the plant epitopes (alpha-1,3-fucose and beta-1,2-xylose), or a large amount of galactose-extended N-glycan structures. Targeting of GalT to different Golgi subcompartments. The sequence corresponding to the catalytic region (amino acid residues 123-397) of the human GalT precursor is fused via a SpeI restriction site to the first 77 amino acids from Nicotiana tabacum GNTI, corresponding to the CTS domain