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2.4.1.214: glycoprotein 3-alpha-L-fucosyltransferase

This is an abbreviated version!
For detailed information about glycoprotein 3-alpha-L-fucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.214

Reaction

GDP-beta-L-fucose
+
N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein]
=
GDP
 +
N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc]-L-asparaginyl-[protein]

Synonyms

alpha 1,3-fucosyltransferase VI, alpha(1,3)-fucosyltransferase VII, alpha(1,3)-fucosyltransferase-IV, alpha(1,3)-fucosyltransferase-VII, alpha(1,3)fucosyltransferase, alpha-1,3-fucosyltransferase-VII, alpha1,3-fucosyltransferase, alpha1,3-fucosyltransferase 10, alpha1,3-fucosyltransferase 7, alpha1,3-fucosyltransferase 9, alpha1,3-fucosyltransferase IX, alpha1,3FTs, alpha1-3 fucosyltransferase-VII, alpha3-fucosyltransferase, alpha3-FucT VII, AtFUT11, CEFT-1, CEFT-2, CEFT-3, CEFT-4, CEFT-5, CEFT3, core alpha-(1,3)-fucosyltransferase, core alpha1,3-fucosyltransferase, Fuc-T C3, Fuc-TVII, fucosyltransferase A, fucosyltransferase VII, FucT, FucT 115, FucT 45, FucT VI, FucT VII, FucT-IV, FucT-VII, FucT1, FucTA, FucTB, FucTC, FUT-1, FUT-6, FUT10, FUT11, FUT7, Fut9, GDP-fucose:beta-N-acetylglucosamine (Fuc to (Fuca1-6GlcNAc)-Asn-peptide) alpha1-3-fucosyltransferase, GDP-L-fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase, GDP-L-fuc:N-acetyl-beta-D-glucosaminide alpha1,3-fucosyltransferase, LOC107797347, LOC107830046, NM_001324945, XM_016620229, XM_016657530

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.214 glycoprotein 3-alpha-L-fucosyltransferase

Engineering

Engineering on EC 2.4.1.214 - glycoprotein 3-alpha-L-fucosyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N219A
the mutant retains 1.2% of the wild type activity
N337A
the mutant retains 18.6% of the wild type activity
R226A
the mutation leads to a complete loss of activity
S218A
the mutation completely abolishes the enzyme activity
S253A
the mutant retains 35.6% of the wild type activity
S483A
the mutant retains 32.2% of the wild type activity
T339A
the mutant retains 12.1% of the wild type activity
T422A
the mutant retains 36.5% of the wild type activity
Y243A
the mutant retains 9.8% of the wild type activity
D303E
the mutant retains 23.8% of the wild type activity
N194Q
glycosylation site mutant with less than 1% of wild type activity
S243A
the mutant retains 16.2% of the wild type activity
T361A
the mutant retains 24.3% of the wild type activity
E249A
-
FucT 45, no activity
E249D
-
FucT 45, no activity
E249Q
-
FucT 45, no activity
E95A
-
FucT 45, no activity
E95D
-
FucT 45, no activity
K250A
-
FucT 45, no activity
L202M
-
to introduce methionine
N240D
-
FucT 45, active
R195A
-
FucT 45, no activity
R195K
-
FucT 45, active
Y246A
-
FucT 45, active
Y246F
-
FucT 45, active
E249A
-
FucT 45, no activity
-
E249D
-
FucT 45, no activity
-
E249Q
-
FucT 45, no activity
-
K250A
-
FucT 45, no activity
-
Y246A
-
FucT 45, active
-
E247A
-
site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, below 1% activity compared to the wild-type FucT VI
E257A
-
site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, below 1% activity compared to the wild-type FucT VI
F242A
-
site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, below 1% activity compared to the wild-type FucT VI
F246A
-
site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, 7% activity compared to the wild-type FucT VI
K241A
-
site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, low expression level in COS-7 cells, below 1% activity compared to the wild-type FucT VI
K258A
-
site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, inactive mutant
L244A
-
site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, 7% activity compared to the wild-type FucT VI
N248A
-
site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, below 1% activity compared to the wild-type FucT VI
S249A
-
site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, 57% activity compared to the wild-type FucT VI
T256A
-
site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, below 1% activity compared to the wild-type FucT VI
Y240A
-
site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, 14% activity compared to the wild-type FucT VI
Y254A
-
site-directed mutagenesis of a residue from the conserved alpha 1,3 FucT motif, inactive mutant
additional information