2.4.1.210: limonoid glucosyltransferase
This is an abbreviated version!
For detailed information about limonoid glucosyltransferase, go to the full flat file.
Reaction
Synonyms
CitLGT-1, CitLGT-2, CrLGT, cuGT, LGT, LGTase, limonoid glucosyltransferase, limonoid GTase, limonoid UDP glucosyltransferase, limonoid UDP-glucosyltransferase, SLLGT, UDP-D-glucose:limonoid glucosyltransferase, UDP-glucose:limonoid glucosyltransferase
ECTree
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Inhibitors
Inhibitors on EC 2.4.1.210 - limonoid glucosyltransferase
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1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
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modifies tryptophan residues
diethyldicarbonate
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modifies histidine residues and inactivates the enzyme, pseudo first order kinetics. When the histidine modification is reversed by hydroxylamine treatment, 79% of the activity is restored
N-bromosuccinimide
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modifies tryptophan residues and inactivates the enzyme, loss of LGTase activity by NBS treatment is partially protected by pre-incubating the enzyme with excess limonin
additional information
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inactivation of the enzyme following modification of carboxyl and imidazole moieties is a consequence of a loss in substrate binding and catalysis in the glucosyltransfer reaction. No inhibition by serine modifiying diisopropyl fluorophosphate and cysteine modifying 4-chloromercuribenzoate and iodoacetamide
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