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2.4.1.14: sucrose-phosphate synthase

This is an abbreviated version!
For detailed information about sucrose-phosphate synthase, go to the full flat file.

Word Map on EC 2.4.1.14

Reaction

UDP-alpha-D-glucose
+
D-fructose 6-phosphate
=
UDP
 +
sucrose 6F-phosphate

Synonyms

At5g20280, AtSPS, More, SPS, SPS A, SPS1, SPS11, Sps2, SPS4, SPS6, SPS8, SPSA, SpsB, SPSII, sucrose 6-phosphate synthase, sucrose phosphate synthase, sucrose phosphate synthase 4, sucrose phosphate synthase A, sucrose phosphate synthase B, sucrose phosphate synthetase, sucrose phosphate-uridine diphosphate glucosyltransferase, sucrose-P synthase, sucrose-phosphate synthase B, sucrosephosphate-UDP glucosyltransferase, UDP-glucose-fructose-phosphate glucosyltransferase, UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase, uridine diphosphoglucose-fructose phosphate glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.14 sucrose-phosphate synthase

Cloned

Cloned on EC 2.4.1.14 - sucrose-phosphate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
analysis of transient expression of SPS genes, functional significance of the various cis-acting regulatory elements present in banana SPS promoter in regulating SPS expression during ripening
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antisence transformants with reduced activity were produced
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expressed in Brachypodium distachyon inbred line Bd21
expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli BL21(DE3) cells and Nicotiana tabacum
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expressed in Medicago sativa
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expressed in Nicotiana tabaccum cultivar Yunyan 85
expressed in tomato
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expressed in tomato and Escherichia coli, both reveal active enzyme, reduced amount of starch in leaves of transformed tomato
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expression in Escherichia coli
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expression in Escherichia coli and tobacco, active enzyme
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expression in Escherichia coli. The Synechocystis sps gene is introduced into tobacco, rice and tomato under the control of constitutive promoters. The Synechocystis SPS protein is expressed at high level. However SPS activities and carbon partitioning in leaves from transgenic and wild-type plants are not significantly different. The purified enzymes have full catalytic activity. It is proposed that some other protein in plant cells binds to the Synechocystis SPS resulting in inhibition of the enzyme
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expression in Populus alba x Populus grandidentata causing an altered phenotype compared to the wild-type trees with altered timing of bud flush and leaf senescence. Tree height and stem diameter are similar to the wild-type, but differences in the length of xylem fibres occur. Elevated concentrations of intracellular sucrose in both leaf and stem tissue of the transgenic trees are associated with a prolonged onset of senescence and an advancement in bud flush in the following spring, phenotypes, overview
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expression of a 26000 Da fragment in Escherichia coli
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gene AtSPS5b, cloned with forward primer SPSa3 and reverse primer SPSb3, transfection via Agrobacterium tumefaciens strain EHA105 into Arabidopsis thaliana seedligs, transient recombinant expression of YFP-tagged enzyme in Nicotiana tabacum leaves and coexpression of sucrose phosphate phosphatase, recombinant expression in Saccharomyces cerevisiae strain MaV203 in a two-hybrid system with sucrose phosphate phosphatase, SPP, EC 3.1.3.24, AtSPP-AtSPS fusion is trabsfectde via Agrobacterium tumefaciens strain EHA105
gene KC-SPS1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree
gene SoSPS1, recombinant expression of N-terminaly His6-tagged wild-type enzyme and truncated mutant enzymes lacking up to 171 residues of the N-terminal region in Escherichia coli strain BL21(DE3), recombinant expression of only the full-length wild-type enzyme with a His8-myc-His8 tag, a TEV cleavage site, and EGFP fusion in Spodoptera frugiperda Sf9 cells, the latter via baculovirus transfection
gene SPS, cloning of SPS promoter region
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gene SPS, qualitative and quantitative realtime PCR analysis, overview
gene sps, quantitative expression analysis by RT-PCR
gene sps, sequence comparisons, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli
gene SPS-1 or SPSA, overexpression in Medicago sativa driven by the constitutive CaMV35S promoter via Agrobacterium tumefaciens strain GV3101 transfection. Overexpression and increased activity of the enzyme in alfalfa is accompanied by early flowering, increased plant growth and an increase in elemental N and protein content when grown under N2-fixing conditions
gene sps1 or spsa1, recombinant expression in Saccharomyces cerevisiae strain CY1905, promoter-reporter gene analyses and quantitative real-time reverse transcription-PCR studies, isozyme expression patterns
gene SPS1, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis
gene SPS1, real-time quantitative RT-PCR analysis of SPS1 expression in wild-type and transgenic plants
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gene SPS1, recombinant expression driven by the CaMV35S promoter in leaves of Nicotiana tabacum via Agrobacterium tumefaciens strain GV3101 transfection method, coexpression with soybean glutamine synthetase
gene SPS11, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis
gene sps2 or spsa2, recombinant expression in Saccharomyces cerevisiae strain CY1905, promoter-reporter gene analyses and quantitative real-time reverse transcription-PCR studies, isozyme expression patterns
gene SPS2, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis
gene sps3 or spsb, recombinant expression in Saccharomyces cerevisiae strain CY1905, promoter-reporter gene analyses and quantitative real-time reverse transcription-PCR studies, isozyme expression patterns
gene SPS6, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis
gene SPS8, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis
gene SPSII, semiquantitative PCR expression analysis
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genetic structure of SPSII genes, sequence comparisons and genetic mapping, phylogenetic tree, expression analysis of the SPSII family, overview
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Nicotiana tabacum cv. Xanthi plants are transformed with an arabidopsis SPS gene under the regulation of the ubiquitously expressed tandem repeat of the 35S cauliflower mosaic virus promoter. All transformed plants have significantly increased stem height, which is ascribed to internode elongation, and greater stem diameters, longer fibers and increased total dry biomass relative to the control plants. Difference in the chemical composition of either the storage or structural carbohydrates of the wild-type and SPS transgenic lines are only minor
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overexpression of maize sucrose-phosphate synthase gene in Nicotiana tabacum increases the sucrose synthesis and carbon assimilation, particularly in older leaves, accelerates the whole plant development and increases the abundance of flowers without substantial changes in the overall shoot biomass
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SPSA and SPSB, DNA and amino acid sequence determination and analysis
transgenic cotton over-producing spinach sucrose phosphate synthase shows enhanced leaf sucrose synthesis and improved fiber quality under controlled environmental conditions