Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.14 extracted from

  • But, S.Y.; Khmelenina, V.N.; Reshetnikov, A.S.; Trotsenko, Y.A.
    Bifunctional sucrose phosphate synthase/phosphatase is involved in the sucrose biosynthesis by Methylobacillus flagellatus KT (2013), FEMS Microbiol. Lett., 347, 43-51.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
Methylobacillus flagellatus
D-fructose 1,6-bisphosphate
-
Methylobacillus flagellatus

Cloned(Commentary)

Cloned (Comment) Organism
gene sps, sequence comparisons, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli Methylobacillus flagellatus

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
Methylobacillus flagellatus
D-Fructose 1-phosphate slight inhibition Methylobacillus flagellatus
D-glucose 6-phosphate
-
Methylobacillus flagellatus
D-ribose 5-phosphate
-
Methylobacillus flagellatus
additional information poor effect by fructose and sucrose Methylobacillus flagellatus
phosphate slight inhibition Methylobacillus flagellatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
sucrose 6F-phosphate pH 6.5, 45°C, recombinant His6-tagged enzyme Methylobacillus flagellatus
1.2
-
D-fructose 6-phosphate pH 6.5, 45°C, recombinant His6-tagged enzyme Methylobacillus flagellatus
1.3
-
UDP-glucose pH 6.5, 45°C, recombinant His6-tagged enzyme, in presence of fructose 1,6-bisphosphate Methylobacillus flagellatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on, optimal at 17.5 mM Methylobacillus flagellatus
NaCl activates, best at 2% Methylobacillus flagellatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84132
-
4 * 84132, sequence calculation Methylobacillus flagellatus
336000
-
gel filtration Methylobacillus flagellatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Methylobacillus flagellatus a bifunctional sucrose phosphate synthase/phosphatase (SPS/SPP) ?
-
?
UDP-glucose + D-fructose 6-phosphate Methylobacillus flagellatus
-
UDP + sucrose 6F-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Methylobacillus flagellatus Q1GY13
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity Methylobacillus flagellatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a bifunctional sucrose phosphate synthase/phosphatase (SPS/SPP) Methylobacillus flagellatus ?
-
?
UDP + sucrose 6F-phosphate
-
Methylobacillus flagellatus UDP-glucose + D-fructose 6-phosphate
-
r
UDP-glucose + D-fructose 6-phosphate
-
Methylobacillus flagellatus UDP + sucrose 6F-phosphate
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 84132, sequence calculation Methylobacillus flagellatus

Synonyms

Synonyms Comment Organism
SPS
-
Methylobacillus flagellatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Methylobacillus flagellatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Methylobacillus flagellatus