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2.3.2.23: E2 ubiquitin-conjugating enzyme

This is an abbreviated version!
For detailed information about E2 ubiquitin-conjugating enzyme, go to the full flat file.

Word Map on EC 2.3.2.23

Reaction

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
+
[E2 ubiquitin-conjugating enzyme]-L-cysteine
=
[E1 ubiquitin-activating enzyme]-L-cysteine
 +
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine

Synonyms

alpha spectrin, ARIH1, At1g78870, At2g02760, CDC34, Cdc34B, Ci0100152677, E2 enzyme, E2 Ub-conjugating enzyme, E2 ubiquitin conjugating enzyme, E2 ubiquitin-conjugating enzyme, E2-20K, E2-25K, E2-conjugating enzyme, E2-ubiquitin conjugating enzyme, FANCT, HOYS7, Mms2, NCUBE1, NEDD8-activating enzyme E1 catalytic subunit, Pex4, Rad6a, RAD6B, Rad6p, SCEI, SUMO E2 conjugating enzyme, SUMO E2 enzyme, SUMO-1-conjugating enzyme Ubc9, SUMO-conjugating enzyme UBC9, UBA2, Uba3, UBA6-specific E2 enzyme 1, UBC E2, Ubc-18, Ubc1, Ubc11, Ubc13, Ubc2, Ubc35, Ubc3B, Ubc4, Ubc4/5, Ubc5a, Ubc6, UBC6e, UBC7, Ubc8, Ubc9, UbcA1, UBCE4, UbcH10, UBCH2, UbcH3, UbcH5A, UbcH5B, UbcH5C, UbcH5D, UbcH6, UbcH7, UbcH8, UbcH9, UbcM2, UbcM4, Ube2, UBE2A, UBE2B, UBE2C, UBE2D, UBE2D1, ube2d1b, UBE2D2, UBE2D3, UBE2D4, UBE2E1, UBE2E2, UBE2E3, Ube2G, UBE2G1, UBE2G2, UBE2H, Ube2I, Ube2J1, UBE2J2, UBE2K, UBE2L3, UBE2L6, UBE2N, Ube2NL, UBE2Q1, UBE2Q2, UBE2QL, UBE2QL1, Ube2r, UBE2R1, UBE2R2, UBE2S, UBE2T, UBE2U, Ube2V, UBE2V1, UBE2V2, UBE2W, UBE2Z, ubiquitin-conjugating enzyme, ubiquitin-conjugating enzyme (E2), ubiquitin-conjugating enzyme E2, ubiquitin-conjugating enzyme E2 B, ubiquitin-conjugating enzyme E2 D1, ubiquitin-conjugating enzyme E2 G1, ubiquitin-conjugating enzyme E2 Z, ubiquitin-conjugating enzyme E2-20 kDa, ubiquitin-conjugating enzyme E2T, ubiquitination enzyme, Uev1, Uev1A, Uev2, USE1, VvUBC

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.23 E2 ubiquitin-conjugating enzyme

Purification

Purification on EC 2.3.2.23 - E2 ubiquitin-conjugating enzyme

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Superdex 75 gel filtration
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recombinant enzyme, N-terminally His-tagged, subcloned in Escherichia coli BL21(DE2), via pET28 vector, purified using Ni-NTA column and HiLoad 16/60 Superdex 75 gel filtration column, purity 95% after two steps
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recombinant GST- or His6-tagged enzyme UBE2R1 from Escherichia coli strain Rosetta (DE3) by glutathione or nickel affinity chromatography, respectively, followed by tag cleavage through TEV protease, and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) Codon Plus by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, followed by tag cleavage by TEV protease, dialysis, and gel filtration
recombinant His6-tagged enzyme UBE2Z from Escherichia coli strain BL21(DE3) by metal affinity chromatography, dialysis, tag cleavage through HRV 3C protease, followed by anion exchange chromatography and dialysis, gel filtration and ultrafiltration
recombinant protein, Ni-NTA column
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