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Literature summary for 2.3.2.23 extracted from

  • Qi, C.; Li, D.F.; Feng, L.; Hou, Y.; Sun, H.; Wang, D.C.; Liu, W.
    Biochemical and structural characterization of a novel ubiquitin-conjugating enzyme E2 from Agrocybe aegeria reveals Ube2w family-specific properties (2015), Sci. Rep., 5, 16056.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.23

Crystallization (Commentary)

Crystallization (Comment) Organism
using 20% (w/v) PEG 3350, 0.2 M sodium acetate, and 0.1 M Bis-Tris, pH 6.5 Cyclocybe aegerita

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18400
-
 2 * 18400, SDS-PAGE Cyclocybe aegerita

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine Cyclocybe aegerita
-
 [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Cyclocybe aegerita
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Cyclocybe aegerita

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 Cyclocybe aegerita [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 18400, SDS-PAGE Cyclocybe aegerita

Synonyms

Synonyms Comment Organism
UbcA1
-
 Cyclocybe aegerita
ubiquitin-conjugating enzyme E2
-
 Cyclocybe aegerita