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2.3.2.20: cyclo(L-leucyl-L-phenylalanyl) synthase

This is an abbreviated version!
For detailed information about cyclo(L-leucyl-L-phenylalanyl) synthase, go to the full flat file.

Word Map on EC 2.3.2.20

Reaction

L-leucyl-tRNALeu
+
L-phenylalanyl-tRNAPhe
=
tRNALeu
 +
tRNAPhe
 +
cyclo(L-leucyl-L-phenylalanyl)

Synonyms

AlbC, BcmA, CDP, CDPS, CDPS 22, CDPS39, cFL synthase, cyclodipeptide synthase, cyclodipeptide synthases, Fdum-CDPS, Nbra-CDPS, NcdA, NCTC11370_02388, Ndas_1148, NozA, O3I_025450, Rgry-CDPS, RICGR_0139

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.20 cyclo(L-leucyl-L-phenylalanyl) synthase

Cloned

Cloned on EC 2.3.2.20 - cyclo(L-leucyl-L-phenylalanyl) synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene albC, recombinant expression in Escherichia coli strain BL21AI, subcloning in Escherichia coli strain DH5alpha
gene albC, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized gene in Escherichia coli strain M15
gene bmcA, recombinant expression of the bcm cluster in heterologous host Streptomyces coelicolor, resulting in production of bicyclomycin
-
gene NCTC11370_02388, recombinant expression in Escherichia coli strain BL21AI, subcloning in Escherichia coli strain DH5alpha
gene O3I_025450, recombinant expression in Escherichia coli strain BL21AI, subcloning in Escherichia coli strain DH5alpha
gene RICGR_0139, recombinant expression in Escherichia coli strain BL21AI, subcloning in Escherichia coli strain DH5alpha