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2.3.1.28: chloramphenicol O-acetyltransferase

This is an abbreviated version!
For detailed information about chloramphenicol O-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.28

Reaction

acetyl-CoA
+
chloramphenicol
=
CoA
 +
chloramphenicol 3-acetate

Synonyms

acetyltransferase, chloramphenicol, CAP acetyltransferase, CAT, CAT I, CAT II, CAT III, cat-86, CATC, CATI, chloramphenicol acetylase, chloramphenicol acetyltransferase, chloramphenicol acetyltransferase B2, chloramphenicol transacetylase, Pacat, Tn9 ca

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.28 chloramphenicol O-acetyltransferase

Temperature Stability

Temperature Stability on EC 2.3.1.28 - chloramphenicol O-acetyltransferase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53
-
0.1 mM chloramphenicol and 0.1 mM acetyl-CoA, 50 mM Tris-HCl buffer, pH 7.8, half-life: 100 min
55
-
30 min, 82% loss of activity, wild-type enzyme
60
-
nearly complete inactivation within 10 min
68.3
-
melting temperature, mutant F97W
70
-
15 min, 40% loss of activity of enzyme variant from plasmid pSCS6, 45% loss of activity of enzyme variant pSCS7
72
-
melting temperature, wild-type