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2.3.1.28: chloramphenicol O-acetyltransferase

This is an abbreviated version!
For detailed information about chloramphenicol O-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.28

Reaction

acetyl-CoA
+
chloramphenicol
=
CoA
+
chloramphenicol 3-acetate

Synonyms

acetyltransferase, chloramphenicol, CAP acetyltransferase, CAT, CAT I, CAT II, CAT III, cat-86, CATC, CATI, chloramphenicol acetylase, chloramphenicol acetyltransferase, chloramphenicol acetyltransferase B2, chloramphenicol transacetylase, Pacat, Tn9 ca

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.28 chloramphenicol O-acetyltransferase

Purification

Purification on EC 2.3.1.28 - chloramphenicol O-acetyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ELP (elastin-like polypeptide) fusion tag technique to purify ultra-low levels of chloramphenicol acetyltransferase-ELP fusion protein
-
enzyme variant A
-
enzyme variant B
-
enzyme variant C and D
-
plasmid Rms418 is transferred from Vibrio anguillarum to E. coli K-12
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus(DE3)-RIPL by nickel affinity chromatography and gel filtration
-