2.3.1.246: 3,5-dihydroxyphenylacetyl-CoA synthase

This is an abbreviated version!
For detailed information about 3,5-dihydroxyphenylacetyl-CoA synthase, go to the full flat file.

Reaction

4 malonyl-CoA =

+ 3 CoA + 4 CO2 +

Synonyms

dpgA

ECTree

2 Transferases

2.3 Acyltransferases

2.3.1 Transferring groups other than aminoacyl groups

2.3.1.246 3,5-dihydroxyphenylacetyl-CoA synthase

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Results	in table
11	AA Sequence
3	Cloned(Commentary)
2	General Information
2	kcat/KM [mM/s]
2	KM Value [mM]
1	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
5	Organism
2	Pathway
4	pH Optimum
5	Protein Variants
3	Purification (Commentary)
1	Reaction
5	Reference
5	Substrates and Products (Substrate)
1	Subunits
5	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Turnover Number [1/s]

Engineering

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Engineering on EC 2.3.1.246 - 3,5-dihydroxyphenylacetyl-CoA synthase

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C160A

Amycolatopsis mediterranei

kcat/Km for malonyl-CoA is 0.16% compared to the kcat/Km-value of the wild-type enzyme. The mutant shows an increased partition ratio for malonyl-CoA decarboxylation to acetyl-CoA vs condensation to 3,5-dihydroxyphenylacetyl-CoA, reflecting more uncoupling in the mutant enzyme

729197

C160S

Amycolatopsis mediterranei

kcat/Km for malonyl-CoA is 2.3% compared to the kcat/Km-value of the wild-type enzyme

729197

C190A

Amycolatopsis mediterranei

mutant retains wild-type activity

729197

H296A

Amycolatopsis mediterranei

mutant has a 6fold drop in kcat

729197

C160A/C190A

Amycolatopsis orientalis

formation of (3,5-dihydroxyphenylacetyl)-CoA is barely detectable

729594