2.3.1.207: beta-ketodecanoyl-[acyl-carrier-protein] synthase
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Synonyms
beta-ketoacyl synthase, FabY, PA3286 protein
ECTree
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Engineering
Engineering on EC 2.3.1.207 - beta-ketodecanoyl-[acyl-carrier-protein] synthase
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additional information
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gene fabY is replaced with fabH (pTMT123) or deleted (pTMT124) in Pseudomonas aeruginosa strain PAO1. The fabY deletion strain grows slowly in comparison to the fabHEc exchange control without C10 supplementation, synthetic lethal analysis for fabY with KASIII domain fabH orthologues and fatty acid rescue, overview. The fabY deletion is synthetic lethal when introduced into the KASIII domain knockout strain TMT16. Synthetic lethal relationship between PA3286 and fabY indicates shared acetyl-CoA:malonyl-ACP condensing activity. PA3286 cross complements fabH and confers a fatty acid shunt in Escherichia coli