2.1.1.267: flavonoid 3',5'-methyltransferase
This is an abbreviated version!
For detailed information about flavonoid 3',5'-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.267
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2.1.1.267
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anthocyanins
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flavonols
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petals
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petunia
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o-methylation
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malvidin
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delphinidin
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b-ring
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hybrida
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polymethoxylated
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madagascar
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eriodictyol
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fragrant
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cyclamen
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cyanidin
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dimethoxylated
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nonmethylated
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periwinkle
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3-o-glucoside
- 2.1.1.267
- anthocyanins
- flavonols
- petals
- petunia
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o-methylation
- malvidin
- delphinidin
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b-ring
- hybrida
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polymethoxylated
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madagascar
- eriodictyol
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fragrant
- cyclamen
- cyanidin
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dimethoxylated
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nonmethylated
- periwinkle
- 3-o-glucoside
Reaction
Synonyms
3'-OMT, A3'5'OMT, anthocyanin methyltransferase, anthocyanin O-methyltransferase, AnthOMT, AOMT, AOMT1, AOMT2, AOMT3, CdFOMT1, CdFOMT3, CdFOMT4, CdFOMT5, CdFOMT6, CrCOMT2, CrOMT2, EC 2.1.1.149, flavonoid 3',5'-O-dimethyltransferase, flavonoid O-methyltransferase, flavonoid-O-methyltransferase, FOMT1, FOMT3, FOMT4, FOMT5, FOMT6, methyltransferase, flavonoid, Mg2+-independent O-methyltransferase, More, NmAMT6, OMT2, OMT3, PsAOMT, PtAOMT, ROMT-15, ROMT-17, S-adenosylmethionine: anthocyanin 3'5'-methyltransferase, SlOMT3, ThAOMT, TMT5, type II OMT
ECTree
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Engineering
Engineering on EC 2.1.1.267 - flavonoid 3',5'-methyltransferase
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D168L
D194L
D209L
D234L
E112L
E69L
N195I
N235I
L87A
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site-directed mutagenesis, the mutant shows highly decreased catalytic efficency, the observed in vitro catalytic efficiency of PtAOMT-L87R mutant is equal to that of Paeonia tenuifolia PtAOMT wild-type
L87R
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site-directed mutagenesis, the mutant shows highly decreased catalytic efficency, the observed in vitro catalytic efficiency of PtAOMT-L87R mutant is equal to that of Paeonia tenuifolia PtAOMT wild-type
G13E
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site-directed mutagenesis, the mutant shows increased activity compared to wild-type
R87L
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site-directed mutagenesis, the mutant shows highly increased catalytic efficency, the observed in vitro catalytic efficiency of PtAOMT-R87L mutant is equal to that of Paeonia suffruticosa PsAOMT wild-type
T205R
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site-directed mutagenesis, the mutant shows increased activity compared to wild-type
T85A
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site-directed mutagenesis, the mutant shows increased activity compared to wild-type
additional information
E112L
mutation results in 40% loss of activity (with quercetin as substrate)
E69L
mutation results in 14% loss of activity (with quercetin as substrate)
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silencing of AnthOMT in hypocotyl tissue, several hypocotyls of three lines (57, 85 and 87) show an anthocyanin profile that is different to that of the wild-type, and show a strong reduction in malvidin-type anthocyanins, while other lines (64, 66 and 71) show only wild-type anthocyanin profiles
additional information
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construction of a multifunctional flavonoid O-methyltransferase fusing tomato 3'-O-methyltransferase OMT3 and Oryza sativa naringenin 7-O-methyltransferase NOMT. The OMT3/NOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual OMT3 and NOMT. The OMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using OMT3/NOMT-transformed Escherichia coli generates corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-Omethyltaxifolin, respectively
additional information
construction of a multifunctional flavonoid O-methyltransferase fusing tomato 3'-O-methyltransferase OMT3 and Oryza sativa naringenin 7-O-methyltransferase NOMT. The OMT3/NOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual OMT3 and NOMT. The OMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using OMT3/NOMT-transformed Escherichia coli generates corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-Omethyltaxifolin, respectively
additional information
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generation of a multifunctional FOMT fusing a 3'-OMT (SlOMT3, EC 2.1.1.267) and a 7-OMT (OsNOMT, EC 2.1.1.82). The SlOMT3/OsNOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual SlOMT3 and OsNOMT. The SlOMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively, suggesting that the fusion enzyme can sequentially methylate flavonoids into di-O-methylflavonoids. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using SlOMT3/OsNOMT-transformed Escherichia coli generated corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-O-methyltaxifolin, respectively. These results indicate that dimethoxyflavonoids may be efficiently produced from non-methylated flavonoid precursors through a one-step biotransformation using the engineered Escherichia coli harboring the SlOMT3/OsNOMT fusion gene
additional information
generation of a multifunctional FOMT fusing a 3'-OMT (SlOMT3, EC 2.1.1.267) and a 7-OMT (OsNOMT, EC 2.1.1.82). The SlOMT3/OsNOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual SlOMT3 and OsNOMT. The SlOMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively, suggesting that the fusion enzyme can sequentially methylate flavonoids into di-O-methylflavonoids. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using SlOMT3/OsNOMT-transformed Escherichia coli generated corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-O-methyltaxifolin, respectively. These results indicate that dimethoxyflavonoids may be efficiently produced from non-methylated flavonoid precursors through a one-step biotransformation using the engineered Escherichia coli harboring the SlOMT3/OsNOMT fusion gene
additional information
flower color modification in Rosa hybrida by expressing the S-adenosylmethionine: anthocyanin 3',5'-O-methyltransferase gene from Torenia hybrida, phenotypes of T-DNA insertion transgenic plants, overview