Cloned (Comment) | Organism |
---|---|
gene AOMT, cloned from a cDNA library derived from Torenia hybrida petals that mainly accumulated malvidin type anthocyanins using the petunia A3'OMT cDNA as a probe, phylogenetic analysis and tree. Functional recombinant expression in Nierembergia sp. NB18, the petals of which naturally accumulate anthocyanins derived from delphinidin. The resultant transgenic petals produce methylated anthocyanins, based on malvidin and petunidin, in addition to delphinidin. Transgenic rose petals of Rosa hybrida cv. WKS124 expressing both a pansy flavonoid 3',5'-hydroxylase (F3'5'H) and the Torenia A3'5'OMT genes accumulated methylated anthocyanins based upon malvidin, petunidin, and peonidin, which comprise up to 88% of the total anthocyanidins, and their magenta color is more brilliant than that of the petals that accumulate delphinidin type anthocyanins by expressing the F3'5'H gene alone. Agrobacterium tumefaciens strain AGL0 transfection method. Recombinant expression of His-tagged enzyme in Escherichia coli | Torenia hybrid cultivar |
Protein Variants | Comment | Organism |
---|---|---|
additional information | flower color modification in Rosa hybrida by expressing the S-adenosylmethionine: anthocyanin 3',5'-O-methyltransferase gene from Torenia hybrida, phenotypes of T-DNA insertion transgenic plants, overview | Torenia hybrid cultivar |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0659 | - |
cyanidin 3-O-glucoside | pH 7.5, 37°C, recombinant enzyme | Torenia hybrid cultivar | |
0.089 | - |
delphinidin 3,5-O-diglucoside | pH 7.5, 37°C, recombinant enzyme | Torenia hybrid cultivar | |
0.103 | - |
delphinidin 3-O-glucoside | pH 7.5, 37°C, recombinant enzyme | Torenia hybrid cultivar | |
0.123 | - |
cyanidin 3,5-O-diglucoside | pH 7.5, 37°C, recombinant enzyme | Torenia hybrid cultivar |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Torenia hybrid cultivar | A0A090BN67 | cv. Summerwave Blue | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Torenia hybrid cultivar |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Torenia hybrid cultivar | - |
petal | - |
Torenia hybrid cultivar | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cyanidin 3,5-O-diglucoside | - |
Torenia hybrid cultivar | S-adenosyl-L-homocysteine + peonidin 3,5-O-diglucoside | - |
? | |
S-adenosyl-L-methionine + cyanidin 3-O-glucoside | - |
Torenia hybrid cultivar | S-adenosyl-L-homocysteine + peonidin 3-O-glucoside | - |
? | |
S-adenosyl-L-methionine + cyanidin 3-O-glucoside-5-O-coumaroylglucoside | - |
Torenia hybrid cultivar | S-adenosyl-L-homocysteine + peonidin 3-O-glucoside-5-O-coumaroylglucoside | - |
? | |
S-adenosyl-L-methionine + delphinidin 3,5-O-diglucoside | - |
Torenia hybrid cultivar | S-adenosyl-L-homocysteine + petunidin 3,5-O-diglucoside | - |
? | |
S-adenosyl-L-methionine + delphinidin 3-O-glucoside | - |
Torenia hybrid cultivar | S-adenosyl-L-homocysteine + petunidin 3-O-glucoside | - |
? | |
S-adenosyl-L-methionine + delphinidin 3-O-glucoside-5-O-coumaroylglucoside | - |
Torenia hybrid cultivar | S-adenosyl-L-homocysteine + petunidin 3-O-glucoside-5-O-coumaroylglucoside | - |
? | |
S-adenosyl-L-methionine + petunidin 3,5-O-diglucoside | - |
Torenia hybrid cultivar | S-adenosyl-L-homocysteine + malvidin 3,5-O-diglucoside | - |
? | |
S-adenosyl-L-methionine + petunidin 3-O-glucoside | - |
Torenia hybrid cultivar | S-adenosyl-L-homocysteine + malvidin 3-O-glucoside | - |
? | |
S-adenosyl-L-methionine + petunidin 3-O-glucoside-5-O-coumaroylglucoside | - |
Torenia hybrid cultivar | S-adenosyl-L-homocysteine + malvidin 3-O-glucoside-5-O-coumaroylglucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
A3'5'OMT | - |
Torenia hybrid cultivar |
AOMT | - |
Torenia hybrid cultivar |
S-adenosylmethionine: anthocyanin 3'5'-methyltransferase | UniProt | Torenia hybrid cultivar |
ThAOMT | - |
Torenia hybrid cultivar |
TMT5 | - |
Torenia hybrid cultivar |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Torenia hybrid cultivar |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Torenia hybrid cultivar |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Torenia hybrid cultivar |
General Information | Comment | Organism |
---|---|---|
evolution | the torenia A3'5'OMT shares 52-72% amino acid sequence identity with other AOMTs and belongs to the group A1 methyltransferase family that also includes caffeoyl CoA O-methyltransferase | Torenia hybrid cultivar |
metabolism | partial overview over the anthocyanin biosynthetic pathway of Torenia hybrida | Torenia hybrid cultivar |
additional information | anthocyanidin analysis in Nierembergia petals expressing the Torenia A3'5'OMT gene, overview | Torenia hybrid cultivar |