2.1.1.266: 23S rRNA (adenine2030-N6)-methyltransferase
This is an abbreviated version!
For detailed information about 23S rRNA (adenine2030-N6)-methyltransferase, go to the full flat file.
Reaction
Synonyms
23S rRNA methyltransferase, adenine-N6 methyltransferase, m6A2030 methyltransferase, ribosomal RNA large subunit methyltransferase J, rlmJ, rRNA m6A methyltransferase, YhiR, YhiR protein
ECTree
2.1.1.266 23S rRNA (adenine2030-N6)-methyltransferaseAdvanced search results
results (
results (Crystallization
Crystallization on EC 2.1.1.266 - 23S rRNA (adenine2030-N6)-methyltransferase
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CRYSTALLIZATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
purified recombinant C-terminally His6-tagged enzyme, sitting drop vapour diffusion method, mixing of 0.0015 ml of protein solution with an equal amount of reservoir solution containing 0.2 M sodium sulfate decahydrate, 0.1 M Tris-HCl pH 8.5, 30% w/v PEG 4000, and equilibration against 0.08 ml of reservoir solution, 8 days, 20°C, X-ray diffraction strucure determination and analysis at 1.8 A resolution
purified recombinant enzyme in apoform, in complex with the cofactor S-adenosyl-L-methionine, or with product S-adenosyl-L-homocysteine plus substrate analogue AMP, X-ray diffraction structure determination and analysis at 1.85-2.0 A resolution, enzyme apostructure analysis using PDB ID 2OO3, rigid-body refinement of the RlmJAPO structure against the RlmJSAM data
