Literature summary for 2.1.1.266 extracted from

Punekar, A.S.; Selmer, M.
Purification, crystallization and preliminary X-ray diffraction analysis of the 23S rRNA methyltransferase RlmJ from Escherichia coli (2013), Acta Crystallogr. Sect. F, 69, 1001-1003.

Search for more literature for 2.1.1.266

Cloned(Commentary)

Cloned (Comment)	Organism
gene yhiR, recombinant expresssion of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant C-terminally His6-tagged enzyme, sitting drop vapour diffusion method, mixing of 0.0015 ml of protein solution with an equal amount of reservoir solution containing 0.2 M sodium sulfate decahydrate, 0.1 M Tris-HCl pH 8.5, 30% w/v PEG 4000, and equilibration against 0.08 ml of reservoir solution, 8 days, 20°C, X-ray diffraction strucure determination and analysis at 1.8 A resolution	Escherichia coli

Crystallization (Comment)

Organism

purified recombinant C-terminally His6-tagged enzyme, sitting drop vapour diffusion method, mixing of 0.0015 ml of protein solution with an equal amount of reservoir solution containing 0.2 M sodium sulfate decahydrate, 0.1 M Tris-HCl pH 8.5, 30% w/v PEG 4000, and equilibration against 0.08 ml of reservoir solution, 8 days, 20°C, X-ray diffraction strucure determination and analysis at 1.8 A resolution

Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + adenine2030 in 23S rRNA	Escherichia coli	-	S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P37634	MG1655, gene yhiR	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + adenine2030 in 23S rRNA	-	Escherichia coli	S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA	-	?
S-adenosyl-L-methionine + adenine2030 in 23S rRNA	enzyme RlmJ uses the cofactor S-adenosyl-L-methionine to methylate the exocyclic nitrogen N6 of nucleotide A2030 in 23S rRNA during ribosome assembly in Escherichia coli	Escherichia coli	S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA	-	?

Synonyms

Synonyms	Comment	Organism
23S rRNA methyltransferase	-	Escherichia coli
ribosomal RNA large subunit methyltransferase J	UniProt	Escherichia coli
rlmJ	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	dependent on	Escherichia coli