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2.1.1.231: flavonoid 4'-O-methyltransferase

This is an abbreviated version!
For detailed information about flavonoid 4'-O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.231

Reaction

S-adenosyl-L-methionine
+
a 4'-hydroxyflavanone
=
S-adenosyl-L-homocysteine
 +
a 4'-methoxyflavanone

Synonyms

4'-O-methyltransferase, 4'OMT, B-ring 4'OMT, F 4'-OMT, flavonoid 4'-O-methyltransferase, GerMIII, O-methyltransferase, OMT, regiospecific flavonoid 4'OMT, SOMT-2, SOMT2, soybean O-methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.231 flavonoid 4'-O-methyltransferase

Cloned

Cloned on EC 2.1.1.231 - flavonoid 4'-O-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpression of Oryza sativa ROMT-9, which methylates specifically at the 3'-hydroxyl group of quercetin and SOMT-2, which methylates at the 4'-hydroxyl group, in Escherichia coli
-
DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli, functional coexpression of plant enzymes, e.g. 4-coumaroyl-CoA ligase and chalcone synthase, of different origins catalyzing steps in flavonoid biosynthesis pathways for production of naringenin from p-coumaric acid and synthesis of ponciretin and sakuranetin from naringenin in engineered and transformed Escherichia coli strain BL21(DE3)
expressed in Escherichia coli as a glutathione S-transferase fusion protein. Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC
-
gene CrOMT6, DNA and amino acid sequence determination and analysis, qualitative RT-PCR expression analysis, sequence comparison and phylogenetic tree, expression of His-tagged OMT6 in Escherichia coli
gene gerMIII, GerMIII is present on the biosynthetic gene cluster of dihydrochalcomycin, a macrolide antibiotic that is produced by the Streptomyces sp. KCTC 0041BP, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)