2.1.1.207: tRNA (cytidine34-2'-O)-methyltransferase

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For detailed information about tRNA (cytidine34-2'-O)-methyltransferase, go to the full flat file.

Reaction

Synonyms

C/U34 2-O-methyltransferase, methyltransferase yibK, Trml, tRNA (cytidine/uridine-2'O)-ribose methyltransferase L, tRNA (cytidine34/5-carboxymethylaminomethyluridine34'-O)-methyltransferase, tRNA (Um34/Cm34) methyltransferase, tRNA methyltransferase L, YibK

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.207 tRNA (cytidine34-2'-O)-methyltransferase

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Results	in table
25035	AA Sequence
2	Cloned(Commentary)
5	Cofactor
1	Crystallization (Commentary)
13	General Information
2	KM Value [mM]
1	Metals/Ions
4	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
6	Organism
33	PDB ID
3	pH Optimum
20	Protein Variants
2	Purification (Commentary)
2	Reaction
6	Reference
19	Substrates and Products (Substrate)
5	Subunits
15	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Turnover Number [1/s]

Systematic Name

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Systematic Name on EC 2.1.1.207 - tRNA (cytidine34-2'-O)-methyltransferase

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SYSTEMATIC NAME

IUBMB Comments

S-adenosyl-L-methionine:tRNA (cytidine34/5-carboxymethylaminomethyluridine34-2'-O)-methyltransferase

The enzyme from Escherichia coli catalyses the 2'-O-methylation of cytidine or 5-carboxymethylaminomethyluridine at the wobble position at nucleotide 34 in tRNALeuCmAA and tRNALeucmnm5UmAA. The enzyme is selective for the two tRNALeu isoacceptors and only methylates these when they present the correct anticodon loop sequence and modification pattern. Specifically, YibK requires a pyrimidine nucleoside at position 34, it has a clear preference for an adenosine at position 35, and it fails to methylate without prior addition of the N6-(isopentenyl)-2-methylthioadenosine modification at position 37.