2.1.1.207: tRNA (cytidine34-2'-O)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (cytidine34-2'-O)-methyltransferase, go to the full flat file.
Reaction
Synonyms
C/U34 2-O-methyltransferase, methyltransferase yibK, Trml, tRNA (cytidine/uridine-2'O)-ribose methyltransferase L, tRNA (cytidine34/5-carboxymethylaminomethyluridine34'-O)-methyltransferase, tRNA (Um34/Cm34) methyltransferase, tRNA methyltransferase L, YibK
ECTree
2.1.1.207 tRNA (cytidine34-2'-O)-methyltransferaseAdvanced search results
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results (Engineering
Engineering on EC 2.1.1.207 - tRNA (cytidine34-2'-O)-methyltransferase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
K81E
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
R104E
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
R28E
-
site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme
R64E
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
R74E
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y142A
-
site-directed mutagenesis, the mutant is a monomer in contrast to the wild-type enzyme. Mutant Y142A fails to form a complex with tRNA
K81E
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
-
additional information
additional information
generation of trmL+ and trmL- PBAD-rpoS990-lacZ translational fusions (in rssB- backgrounds) cells, executed for PBAD-rpoS990-lacZ translational fusions with rpoS UUA-Leu CUX-Leu mutations (leu*1)-strains KMT36002 and KMT36010, for rpoS UUG-Leu to CUX-leu mutation (leu*2)-strains KMT37002 and KMT37010, and for rpoS with both UUA-Leu to CUX-Leu and UUG-Leu to CUX-Leu mutations (leu*3)-strains KMT33001 and KMT33013, overview. Comparison of expression of PBAD-rpoS990-lacZ translational fusions carrying wild-type UUX leucine codons with derivatives in which UUX codons are changed to CUX codons, in the presence and absence of TrmL or TusA. The absence of these proteins, and therefore presumably the modifications they catalyze, both abolish PBAD-rpoS990-lacZ translation activity. UUX-Leu to CUX-Leu codon mutations in rpoS suppress the trmL requirement for PBAD-rpoS990-lacZ expression
additional information
-
generation of trmL+ and trmL- PBAD-rpoS990-lacZ translational fusions (in rssB- backgrounds) cells, executed for PBAD-rpoS990-lacZ translational fusions with rpoS UUA-Leu CUX-Leu mutations (leu*1)-strains KMT36002 and KMT36010, for rpoS UUG-Leu to CUX-leu mutation (leu*2)-strains KMT37002 and KMT37010, and for rpoS with both UUA-Leu to CUX-Leu and UUG-Leu to CUX-Leu mutations (leu*3)-strains KMT33001 and KMT33013, overview. Comparison of expression of PBAD-rpoS990-lacZ translational fusions carrying wild-type UUX leucine codons with derivatives in which UUX codons are changed to CUX codons, in the presence and absence of TrmL or TusA. The absence of these proteins, and therefore presumably the modifications they catalyze, both abolish PBAD-rpoS990-lacZ translation activity. UUX-Leu to CUX-Leu codon mutations in rpoS suppress the trmL requirement for PBAD-rpoS990-lacZ expression
