1.5.3.5: (S)-6-hydroxynicotine oxidase
This is an abbreviated version!
For detailed information about (S)-6-hydroxynicotine oxidase, go to the full flat file.
Word Map on EC 1.5.3.5
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1.5.3.5
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arthrobacter
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6-hydroxy-d-nicotine
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nicotinovorans
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oxidans
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flavin
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fad
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flavoenzyme
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nicotine-degrading
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monoamine
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flavoproteins
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hydride
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6-hydroxypseudooxynicotine
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dehydrogenation
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pseudooxynicotine
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d-specific
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pyrrolidine
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dithionite-reduced
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carbon-carbon
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self-formed
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fad-binding
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s-nicotine
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l-enantiomer
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fitzpatrick
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carbon-nitrogen
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isoalloxazine
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6-hdno
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substrate-reduced
- 1.5.3.5
- arthrobacter
- 6-hydroxy-d-nicotine
- nicotinovorans
- oxidans
- flavin
- fad
-
flavoenzyme
-
nicotine-degrading
-
monoamine
- flavoproteins
-
hydride
- 6-hydroxypseudooxynicotine
-
dehydrogenation
- pseudooxynicotine
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d-specific
- pyrrolidine
-
dithionite-reduced
-
carbon-carbon
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self-formed
-
fad-binding
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s-nicotine
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l-enantiomer
-
fitzpatrick
-
carbon-nitrogen
- isoalloxazine
- 6-hdno
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substrate-reduced
Reaction
Synonyms
6-HLNO, 6-hydroxy-L-nicotine oxidase, 6-hydroxy-L-nicotine:oxygen oxidoreductase, 6HLNO, flavoprotein nicotine oxidoreductase, L-6-hydroxynicotine oxidase, LHNO, MAO, NdpB, NicA2, NOX, VppB
ECTree
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KM Value
KM Value on EC 1.5.3.5 - (S)-6-hydroxynicotine oxidase
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0.011
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pH 7.0, 25°C, mutant enzyme K287M
0.02
(S)-6-hydroxynicotine
pH and temperature not specified in the publication
0.2
(S)-6-hydroxynicotine
-
pH 7.0, 25°C, mutant enzyme Y311F
0.34
(S)-6-hydroxynicotine
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pH 7.0, 25°C, mutant enzyme N166A
2.7
(S)-6-hydroxynicotine
mutant R274A/Y311W/C417W, pH 7.4, 22°C
0.064
-
pH 7.0, 25°C, wild-type enzyme
1.1
(S)-6-hydroxynornicotine
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pH 7.0, 25°C, mutant enzyme Y311F
1.2
(S)-6-hydroxynornicotine
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pH 7.0, 25°C, mutant enzyme N166A
0.000114
pH and temperature not specified in the publication
13
(S)-nicotine
mutant R274A/Y311W/C417W, pH 7.4, 37°C
16
(S)-nicotine
mutant R274A/Y311W/C417W, pH 7.4, 22°C
0.19
-
pH 7.0, 25°C, mutant enzyme Y311F, cosubstrate: (S)-6-hydroxynicotine
0.2
O2
-
pH 7.0, 25°C, mutant enzyme N166A, cosubstrate: (S)-6-hydroxynicotine
0.29
O2
-
pH 7.0, 25°C, wild-type enzyme, cosubstrate: (S)-6-hydroxynicotine
0.46
O2
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pH 7.0, 25°C, wild-type enzyme, cosubstrate: (S)-6-hydroxynornicotine